A5069950948- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Plant Virus Research Studies
- Microbial infections and disease research
- Gut microbiota and health
- Anaerobic Digestion and Biogas Production
- Plant and Fungal Interactions Research
- Metabolism and Genetic Disorders
- Antibiotic Resistance in Bacteria
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Metal Extraction and Bioleaching
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Minerals Flotation and Separation Techniques
- Marine Biology and Ecology Research
- Methane Hydrates and Related Phenomena
- Vibrio bacteria research studies
- Mine drainage and remediation techniques
- Environmental DNA in Biodiversity Studies
- vaccines and immunoinformatics approaches
- Peroxisome Proliferator-Activated Receptors
- Agronomic Practices and Intercropping Systems
- Virtual Reality Applications and Impacts
Bangor University
2009-2025
University of Warwick
2014-2023
Plymouth Marine Laboratory
2007-2011
University of Exeter
2009-2011
Dunedin Public Hospital
2011
Significance Metabolism of l -carnitine, a compound abundant in human diet, to trimethylamine by microbiota has been shown promote atherosclerosis and subsequent development heart disease. However, the underpinning molecular biochemical mechanisms remain unknown. In this study, we reveal that previously unidentified Rieske-type protein is responsible for carnitine transformation from microbiota. Knowledge gained our study provides opportunity not only explore Rieske inhibitors preventing...
Existing metagenome datasets from many different environments contain untapped potential for understanding metabolic pathways and their biological impact. Our interest lies in the formation of trimethylamine (TMA), a key metabolite both human health climate change. Here, we focus on bacterial degradation choline, carnitine, glycine betaine
is a clinically important pathogen causing variety of antimicrobial resistant infections in both community and nosocomial settings, particularly pneumonia, urinary tract infection, sepsis. Bacteriophage (phage) therapy being considered primary option for the treatment drug-resistant these types.
Abstract Background The rhizosphere is a hotspot for microbial activity and contributes to ecosystem services including plant health biogeochemical cycling. of viruses, their influence on plant-microbe interactions in the rhizosphere, remains undetermined. Given impact viruses ecology evolution host communities, determining how soil microbiome dynamics crucial build holistic understanding functions. Results Here, we aimed investigate crop management composition bulk soil, root viral...
spp. are causative agents of healthcare-associated infections in patients who immunocompromised and use medical devices. The antibiotic resistance crisis has led to an increase caused by these bacteria, which can develop into potentially life-threatening illnesses if not treated swiftly effectively. Thus, new treatment options for
The current study investigated whether active distraction reduces participants’ experience of pain more than passive during a cold pressor task. In the first experiment, 60 participants were asked to submerge their hand in (2°C) water for as long they could tolerate. They did this with no distraction, and then (electronic gaming system) (television) randomly assigned order. Tolerance time, intensity ratings task absorption measured each condition. A second experiment attempted control...
Coastal environments are dynamic and rapidly changing. Living organisms in coastal known to synthesise large quantities of organic osmolytes, which they use cope with osmotic stresses. The osmolyte glycine betaine (GBT) is ubiquitously found marine biota from prokaryotic Bacteria Archaea plants, protozoa, mammals. In intertidal sediment, GBT represents an important precursor natural methane emissions as much 90% total production these ecosystems can be originated methanogenesis its...
Gammaproteobacteria are important gut microbes but only persist at low levels in the healthy gut. The ecology of environment is poorly understood. Here, we demonstrate that choline an growth substrate for representatives Gammaproteobacteria. Using Proteus mirabilis as a model, investigate role metabolism and cutC gene, encoding choline-trimethylamine lyase, essential degradation to trimethylamine by targeted mutagenesis subsequent complementation experiments. can rapidly utilize enhance rate...
Abstract Coastal saltmarsh sediments represent an important source of natural methane emissions, much which originates from quaternary and methylated amines, such as choline trimethylamine. In this study, we combine DNA stable isotope probing with high throughput sequencing 16S rRNA genes 13C2-choline enriched metagenomes, followed by metagenome data assembly, to identify the key microbes responsible for methanogenesis choline. Microcosm incubation leads formation trimethylamine subsequent...
Background: Phage therapy shows promise for treating antibiotic-resistant Klebsiella infections. Identifying phage depolymerases that target capsular polysaccharides is crucial, as these capsules contribute to biofilm formation and virulence. However, homology-based searches have limitations in novel depolymerase discovery. Objective: To develop a machine learning model identifying ranking potential targeting Klebsiella. Methods: We developed DepoRanker, algorithm rank proteins by their...
Uropathogenic Klebsiella pneumoniae strains, are of major concern with respect to antimicrobial resistance, which has sparked interest in phage therapy as an alternative, or compliment antibiotics. However, very limited vivo data on safety and efficacy for treating UTIs is available. To address this, we developed a model test these parameters cocktail optimised Extended Spectrum Beta-Lactamases (ESBL)-producing UTI strains. Female C57BL/6J mice were infected K. Top52 then received single...
Bacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from variety of environments. However, the diversity coliphages that is present in seawater remains largely unknown, with previous studies focusing on morphological diversity. Here, we isolated characterized three coastal locations United Kingdom Poland. Comparative genomics phylogenetic analysis phage isolates facilitated identification putative new species within genera...
infections, including catheter associated urinary tract are a considerable burden on health care systems. This is due to their difficulty treat, caused by antimicrobial resistance and ability form biofilms. In this study, we investigated the use of
Soil viruses can moderate the roles that their host microbes play in global carbon cycling. However, given most studies investigate surface layer (i.e., top 20 cm) of soil, extent to which this occurs subsurface soil below is unknown. Here, we leveraged public sequencing data interactions between and hosts at depth intervals, down 115 cm. While were detected throughout profile, adaptation varied. Nonetheless, uncovered evidence for potential encourage recycle plant-derived both soils. This...
The detection of viruses (e.g. SARS-CoV-2, norovirus) in wastewater represents an effective way to monitor the prevalence these pathogens circulating within community. However, accurate quantification viral concentrations wastewater, proportional human input, is constrained by a range uncertainties, including (i) dilution sewer network, (ii) degradation RNA during transit, (iii) catchment population and facility use, (iv) efficiency concentration extraction from (v) inhibition amplification...
Microbial metabolism of carnitine to trimethylamine (TMA) in the gut can accelerate atherosclerosis and heart disease, these TMA-producing enzymes are therefore important drug targets. Here, we report first structures oxygenase CntA, an enzyme Rieske family. CntA exists a head-to-tail α3 trimeric structure. The two functional domains (the catalytic mononuclear iron domains) located >40 Å apart same monomer but adjacent neighboring monomers. Structural determination subsequent electron...
Biosulfidogenesis (the generation of hydrogen sulfide by microorganisms) in acidic liquors was investigated using two metabolically-distinct bacteria. One a novel acidophilic sulfate-reducing bacterium (isolate CL4) that grew at pH 3.0 and above glycerol as electron donor, the other type strain Acidithiobacillus ferrooxidans which grown 2.5 (derived from dissolution metallic iron) donor elemental sulfur acceptor. Both bacteria were pH-controlled bioreactors. Isolate CL4 mediated selective...
Summary M ethylocella silvestris , an alphaproteobacterium isolated from a forest soil, can grow on trimethylamine N ‐oxide ( TMAO ) as sole nitrogen source; however, the molecular and biochemical mechanisms underpinning its growth remain unknown. Marker‐exchange mutagenesis enabled identification of several genes involved in metabolism, including sil_3606 permease amino acids‐polyamine APC superfamily, sil_3603 consisting ‐terminal domain unknown function DUF 1989) C...