A5020993038- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Pneumonia and Respiratory Infections
- Cytomegalovirus and herpesvirus research
- MicroRNA in disease regulation
- Renal Diseases and Glomerulopathies
- Organ Donation and Transplantation
- RNA Interference and Gene Delivery
- Renal and related cancers
- Monoclonal and Polyclonal Antibodies Research
- Genetic Associations and Epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Bipolar Disorder and Treatment
- Influenza Virus Research Studies
- Glycosylation and Glycoproteins Research
- Liver Disease and Transplantation
- Neonatal Health and Biochemistry
- Immunodeficiency and Autoimmune Disorders
- Chronic Kidney Disease and Diabetes
- Complement system in diseases
Medical University of Vienna
2015-2022
Intercell (Austria)
2010-2011
Vienna Biocenter
2010-2011
Estimation of genetic risk is crucial for understanding heritable diseases but also transplant outcomes. Polygenic scores (PRSs) are constructed from genome-wide association studies (GWAS) summing an individual's alleles weighted by their effect size. Introducing PRSs into medicine may improve predictions outcomes such as rejection, graft loss or death. This review recent publications highlights the additional variability in explained beyond established clinical models. Four on...
The importance of serine/threonine phosphorylation in signalling and regulation gene expression prokaryotes has been widely recognized. Driven by our interest StkP (the pneumococcal kinase homologue) for vaccine development, we studied its cellular localization. We found that the C-terminally located PASTA (penicillin-binding protein associated) domains, but not N-terminal domain StkP, were on surface live cells grown vitro also accessible to antibodies during infection mice man. Most...
Antigen recognition of allo-peptides and HLA molecules leads to the activation donor-reactive T-cells following transplantation, potentially causing T-cell-mediated rejection (TCMR). Sequencing T-cell receptor (TCR) repertoire can be used track in blood tissue patients after kidney transplantation.In this prospective cohort study, 117 non-sensitized transplant recipients with anti-CD25 induction were included. Peripheral mononuclear cells (PBMCs) sampled pre-transplant at time protocol or...
The streptococcal protein required for cell separation B (PcsB) is predicted to play an important role in peptidoglycan metabolism, based on sequence motifs and altered phenotypes of gene deletion mutant cells exhibiting defects separation. However, no enzymic activity has been demonstrated PcsB so far. By generating strains four different genetic backgrounds we could demonstrate that pcsB not essential survival Streptococcus pneumoniae, but proper division. Deletion displayed cluster...
Steroid pretreatment of deceased donors reduces inflammation in allografts and is recommended by organ procurement guidelines. The impact on long-term graft outcome, however, remains elusive. In this multicenter randomized controlled trial, 306 providing organs for 455 renal transplant recipients were to 1000 mg methylprednisolone or placebo prior (ISRCTN78828338). incidence biopsy-confirmed rejection (Banff>1) at 3 months was 23 (10%) the steroid group 26 (12%) (P = .468). Five-year...
Kidney transplantation is the optimal treatment in end stage renal disease but allograft survival still hampered by immune reactions against allograft. This process driven recognition of allogenic antigens presented to T-cells and their unique T-cell receptor (TCR) via major histocompatibility complex (MHC), which triggers a response potentially leading graft injury. Although system kidney have been studied extensively, subtlety alloreactive responses has impeded sensitive detection at an...
Background Pre-sensitized kidney transplant recipients have a higher risk for rejection following transplantation and therefore receive lymphodepletional induction therapy with anti-human T-lymphocyte globulin (ATLG) whereas non-sensitized patients are induced in many centers basiliximab. The time course of lymphocyte reconstitution regard to the overall donor-reactive T-cell receptor (TCR) specificity remains elusive. Methods/Design Five receiving 1.5-mg/kg ATLG over 7 days five 2 × 20 mg...
Next-generation sequencing (NGS) is nowadays the most used high-throughput technology for DNA sequencing. Among others NGS enables in-depth analysis of immune repertoires. Research in field T cell receptor (TCR) and immunoglobulin (IG) repertoires aids understanding immunological diseases. A main objective V(D)J recombination defining structure specificity repertoire. Accurate processing, evaluation visualization repertoire data important better responses behavior.ImmunoDataAnalyzer (IMDA) a...
Abstract T and B cells are known to play an important role in transplant rejection. Nevertheless, the factors that lead rejection not yet fully understood. We have developed a general bioinformatics pipeline for processing cell receptor (TCR) immunoglobulin (IG) repertoire next-generation sequencing (NGS) data comparing immune properties between multiple groups of samples. Using such can help identify increasing risk allograft after transplantation. Our is implemented Python 3.7. Various...
Within the last decade numerous attempts have been reported in order to expand donor pool and alleviate organ shortage setting of liver transplantation. Aim this blinded randomized controlled trial was evaluate effect steroid pretreatment on outcomes after transplantation.We performed an international, multi-center double-blinded placebo trial. Donors received 1000 mg methylprednisone or before procurement. Primary endpoint were patient graft survival. Secondary end points rate BPAR...