A5048559013- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Polar Research and Ecology
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Actinomycetales infections and treatment
- Plant Disease Resistance and Genetics
- Microbial Metabolism and Applications
- Microbial Natural Products and Biosynthesis
- Protist diversity and phylogeny
- Molecular Biology Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Oral microbiology and periodontitis research
- Epigenetics and DNA Methylation
- Enzyme Production and Characterization
- Microbial Applications in Construction Materials
- Identification and Quantification in Food
- Urinary Tract Infections Management
- Diatoms and Algae Research
- Cancer Research and Treatments
- Ethics in Clinical Research
- Bacillus and Francisella bacterial research
- Botanical Research and Chemistry
Chinese Academy of Sciences
2015-2024
Institute of Microbiology
2015-2024
Microbiology Institute of Shaanxi
2008-2022
Czech Academy of Sciences, Institute of Microbiology
2020-2021
China Tobacco
2018
Dalian Medical University
2015
Abstract Background In gut microbiome studies, the cultured microbial resource plays essential roles, such as helping to unravel functions and host-microbe interactions. Although several major studies have been performed elucidate human microbiota, up 70% of Unified Human Gastrointestinal Genome species not date. Large-scale isolation identification well availability public are imperative for further characterizing functions. Results this study, we constructed a Gut Microbial Biobank (hGMB;...
Abstract Mice are widely used as experimental models for gut microbiome (GM) studies, yet the majority of mouse GM members remain uncharacterized. Here, we report construction a microbial biobank (mGMB) that contains 126 species, represented by 244 strains have been deposited in China General Microorganism Culture Collection. We sequence and phenotypically characterize 77 potential new species propose their nomenclatures. The mGMB includes 22 17 significantly enriched ob/ob wild-type...
Selection of optimal primer pairs in 16S rRNA gene sequencing is a pivotal issue microorganism diversity analysis. However, limited effort has been put into investigation specific sets for analysis the bacterial aging flue-cured tobaccos (AFTs), as well prediction function community. In this study, performance four determining community structure based on sequences AFTs was assessed, and functions genes were predicted using Phylogenetic Investigation Communities by Reconstruction Unobserved...
Numerous mountain glaciers located on the Tibetan Plateau are inhabited by abundant microorganisms. The microorganisms glacier surface exposed to cold, barren and high-ultraviolet radiation environments. Although microbial community composition has been revealed high-throughput sequencing, little is known about microevolution adaptive strategy of certain bacterial populations. In this study, we used a polyphasic approach determine taxonomic status 11 psychrophilic Flavobacterium strains...
A Gram-stain-positive, spore-forming, rod-shaped and aerobic bacterium was isolated from a sediment sample the South Sea in China. The isolate, designated H2(T), grew at 4-45 °C (optimum 37 °C) pH 6-10 7.0). cell-wall peptidoglycan contained meso-diaminopimelic acid. major isoprenoid quinone MK-7 polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine an unknown aminophospholipid. fatty acid iso-C(15 : 0). genomic DNA G+C content strain H2(T)...
A novel Gram-negative, orange-pigmented, slightly halophilic, rod-shaped bacterium, strain T30(T), was isolated from sediment the South China Sea. Phylogenetic analysis showed that T30(T) a member of genus Erythrobacter, sharing highest 16S rRNA gene sequence similarities with Erythrobacter aquimaris JCM 12189(T) (99.5 %) and vulgaris DSM 17792(T) (99.0 %). Levels DNA-DNA relatedness between closely related strains species ranged 14.5 to 56.9 %.The isolate lacked bacteriochlorophyll...
An aerobic, Gram-reaction-positive, non-motile, psychrophilic bacterium, designated strain S6-3(T), was isolated from alpine soil. Cells exhibited a rod-coccus growth cycle and produced yellow pigment. Growth occurred at 1-25 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that S6-3(T) related to members of the genus Arthrobacter, sharing highest sequence similarities with type strains Arthrobacter psychrolactophilus (97.9 %) stackebrandtii (97.6 %). Strain had...
A Gram-negative-staining bacterium, designated strain 25BT, was isolated from a soil sample rice field in Guangxi Province, China, and its taxonomic position investigated by using polyphasic approach. Cells were rod-shaped, non-spore-forming, non-motile strictly aerobic. Strain 25BT grew optimally at 37 °C pH 7.0. The predominant fatty acids of this isolate C18 : 1 ω7c, C19 0 cyclo ω8c C16 0. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence showed that formed...
A Gram-stain-negative, rod-shaped, orange bacterium (strain MDB1-A(T)) was isolated from ice samples collected Midui glacier in Tibet, south-west China. Cells were aerobic and psychrotolerant (growth occurred at 0-25 °C). Phylogenetic analysis based on 16S rRNA gene sequences showed that it a member of the genus Sphingomonas, with its closest relative being Sphingomonas glacialis C16y(T) (98.9% similarity). Q-10 predominant ubiquinone. C17 : 1ω6c summed feature 8 (C18 and/or C18 1ω7c) major...
Strain BZ92r T was isolated from hydrocarbon-contaminated soil. Cells were Gram-negative, aerobic, rod-shaped and cold-adapted (growth at 1–25 °C). The major fatty acids iso-C 15 : 0 (25.6 %), 17 1 ω 9 c (24.9 11 (18.4 %) 3-OH (16.2 %). predominant ubiquinone ubiquinone-8. genomic DNA G+C content 72.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain a member of the genus Luteimonas (94.5–95.2 % sequence similarity). On basis phenotypic, chemotaxonomic...
An actinomycete, designated MS426T, the culture broth of which showed potent antimicrobial activity, was isolated from a deep-sea sediment sample South China Sea. almost-complete sequence 16S rRNA gene strain MS426T determined and aligned with those representatives family Micromonosporaceae available in public databases. Phylogenetic trees were inferred by using three algorithms. Strain formed branch adjacent to Verrucosispora lutea YIM 013T distinct cluster occupied only strains genus...
In this study, two psychrophilic bacterial strains were isolated from the China No. 1 glacier in Xinjiang, north-west China. Cells Gram-positive rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that belonged to genus Cryobacterium . showed they clustered together and are most closely related luteum CGMCC 1.11210 T , flavum 1.11215 psychrophilum 1.4292 psychrotolerans 1.5382 roopkundense 1.10672 The major cellular fatty acids of novel anteiso-C 15 : 0 A, iso-C 16 Both...
Two Gram-stain-negative, rod-shaped bacteria, designated strains Sr22(T) and Sr25(T), were isolated from water of melted ice the China No.1 glacier, Xinjiang Uygur Autonomous Region, China. Cells formed yellow, circular, convex colonies. 16S rRNA gene sequence analysis indicated that Sr25(T) belong to genus Flavobacterium, sharing ≤99.1 ≤99.6 % similarity, respectively, with type recognized species genus. Strain shared highest similarity Flavobacterium tiangeerense CGMCC 1.6847(T) (98.6 %),...
A Gram-stain-negative strain, designated GT1R17T, was isolated from an ervoconite sample collected Gawalong glacier in the Tibet Autonomous Region, PR China. Strain GT1R17T catalase- and oxidase-positive, grew optimally at 20-25°C pH 7.0. The highest level of 16S rRNA gene sequence similarities were found to members genera Nevskia(92.27-93.15 %) Hydrocarboniphaga(91.92-92.96 %). Phylogenetic analyses based on sequences genomic data revealed that strain belonged family Nevskiaceae, but could...
Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted concerns about the need for its reuse. The nicotine TS is considered chemical that to\xic and hazardous to environment.
Strain BZ30(T) was isolated from hydrocarbon-contaminated soil. The Gram-negative, aerobic bacterium psychrophilic and able to grow at temperatures ranging 1 30 °C. predominant cellular fatty acids of strain were summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 0) 2-OH) (37.4 %), C(18 (19.6 C(16 (8.2 C(14 2-OH (8.0 %) (5.0 %). ubiquinone Q-10. Major polar lipids sphingoglycolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine phosphatidylcholine. Spermidine the...
An aerobic, Gram-negative, yellow-pigmented bacterial strain, designated 0533(T), was isolated from frozen soil the China No. 1 glacier. Phylogenetic analysis of 16S rRNA gene sequence demonstrated that strain 0533(T) a member genus Flavobacterium and exhibited 97.1-98.7 % similarity with its nearest phylogenetic neighbours. Strain phenotypic chemotaxonomic characteristics common to Flavobacterium: menaquinone-6 (MK-6) predominant quinone iso-C(15 : 0), C(17 1)ω6c, anteiso-C(15 0) 3-OH, C(15...
A novel psychrotolerant, Gram-negative, motile bacterium, designated strain 328T, was isolated from sea-ice samples collected off the Canadian Basin of Arctic Ocean (7 ° 23′ 14″ N 14 06′ 55″ W). Strain 328T able to grow at 0–37 °C, with optimum growth 25–27 °C. It possessed phosphatidylethanolamine and phosphatidylglycerol as major phospholipids C10 : 0 3-OH (31.78 %), C18 1 ω7c (27.50 %) iso-C15 2-OH and/or C16 (19.22 predominant cellular fatty acids. The DNA G+C content 45.2 mol%....
A Gram-negative, aerobic bacterium, designated strain BZ26 T , was isolated from hydrocarbon-contaminated soil. The psychrophilic, showing good growth over a temperature range of 1–20 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that related to members the genus Dyadobacter and had highest sequence similarity alkalitolerans 12116 (98.1 %), koreensis KCTC 12537 (97.5 %) ginsengisoli Gsoil 043 (97.2 %). Strain MK-7 as major menaquinone summed feature 3 (C 16 : 1 ω 7 c...
Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT 1 -17 T and 2 -1 ) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to genus Nocardioides shared 97.8 % sequence similarity with each other 97.6 98.4 similarity, respectively, kribbensis KSL-2 . Strain grew at 0–35 °C strain 0–25 °C. The major cellular fatty acids of C 17 : ω8 c (32.69 %) iso-C 16 0 (21.74 %). 18...
A non-motile, Gram-stain-negative bacterium, designated R-HLS-17(T), was isolated from the meltwater of Hailuogou Glacier located in Sichuan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate belonged to genus Flavobacterium, with closest relatives being Flavobacterium antarcticum JCM 12383(T) (95.5% sequence similarity), F. omnivorum 11313(T) (95.0%) and fryxellicola LMG 22022(T) (95.2%). Growth occurred at 0-29 °C (optimum, 10-20 °C) pH...
Gram-positive, rod-shaped bacteria, strains Hh8(T), Hh15(T) and Hh40-2, were isolated from the No. 1 glacier in Xinjiang, north-west China. Colonies of strain Hh8(T) orange-yellow, convex round on PYG plates. Strain grew at 0-19 °C pH 5.5-10.5. Hh15(T), which was able to grow 0-20 5.5-12, lemon yellow, Phylogenetic analysis based 16S rRNA gene sequences showed that these three related members genus Cryobacterium. The major cellular fatty acids novel anteiso-C(15:0), iso-C(16:0), iso-C(15:0)...