A5103184488- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Polar Research and Ecology
- Gut microbiota and health
- Plant Pathogens and Fungal Diseases
- Aquaculture disease management and microbiota
- Microbial Metabolism and Applications
- Molecular Biology Techniques and Applications
- Protist diversity and phylogeny
- Probiotics and Fermented Foods
- Microbial Natural Products and Biosynthesis
- Identification and Quantification in Food
- Methane Hydrates and Related Phenomena
- Clostridium difficile and Clostridium perfringens research
- Fungal Biology and Applications
- Actinomycetales infections and treatment
- Plant Disease Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Bacterial Identification and Susceptibility Testing
- Mycotoxins in Agriculture and Food
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Enzyme Production and Characterization
- Yeasts and Rust Fungi Studies
Institute of Microbiology
2016-2025
Chinese Academy of Sciences
2016-2025
Research Institute of Forestry
2025
Chinese Academy of Forestry
2025
Microbiology Institute of Shaanxi
2014-2023
Tianjin University of Commerce
2020-2021
Czech Academy of Sciences, Institute of Microbiology
2020
Henan Agricultural University
2010-2011
University of Chinese Academy of Sciences
2011
BGI Group (China)
2011
Abstract Background In gut microbiome studies, the cultured microbial resource plays essential roles, such as helping to unravel functions and host-microbe interactions. Although several major studies have been performed elucidate human microbiota, up 70% of Unified Human Gastrointestinal Genome species not date. Large-scale isolation identification well availability public are imperative for further characterizing functions. Results this study, we constructed a Gut Microbial Biobank (hGMB;...
Pestafolide A (1), a new reduced spiro azaphilone derivative, and pestaphthalides (2) B (3), two isobenzofuranones, have been isolated form solid cultures of an isolate Pestalotiopsis foedan. The structures these compounds were determined mainly by analysis their NMR spectroscopic data. relative configuration 1−3 was assigned 1H J-values NOESY data, the absolute application CD excitation chirality modified Mosher method. Compounds showed modest antifungal activity.
Aspergillus section (formerly the genus Eurotium) includes xerophilic species with uniseriate conidiophores, globose to subglobose vesicles, green conidia and yellow, thin walled eurotium-like ascomata hyaline, lenticular ascospores. In present study, a polyphasic approach using morphological characters, extrolites, physiological characters phylogeny was applied investigate taxonomy of this section. Over 500 strains from various culture collections new isolates obtained indoor environments...
The epipolythiodioxopiperazines (ETPs) are fungal secondary metabolites proven to trigger both apoptotic and necrotic cell death of tumor cells. However, the underlying mechanism their regulatory role in macroautophagy interplay between autophagy apoptosis initiated by ETPs, remain unexplored. In current work, we found that 11'-deoxyverticillin A (C42), a member induces autophagosome formation, accumulation microtubule-associated protein 1 light chain 3-II (LC3-II ) degradation sequestosome...
Abstract Taxonomic and functional research of microorganisms has increasingly relied upon genome-based data methods. As the depository Global Catalogue Microorganisms (GCM) 10K prokaryotic type strain sequencing project, Type Strain (gcType) published 1049 genomes sequenced by GCM project which are preserved in global culture collections with a valid status. Additionally, information provided through gcType includes >12 000 publicly available genome sequences from GenBank incorporated...
Abstract Mice are widely used as experimental models for gut microbiome (GM) studies, yet the majority of mouse GM members remain uncharacterized. Here, we report construction a microbial biobank (mGMB) that contains 126 species, represented by 244 strains have been deposited in China General Microorganism Culture Collection. We sequence and phenotypically characterize 77 potential new species propose their nomenclatures. The mGMB includes 22 17 significantly enriched ob/ob wild-type...
The phylum Actinobacteria includes important human pathogens like Mycobacterium tuberculosis and Corynebacterium diphtheriae renowned producers of secondary metabolites commercial interest, yet only a small part its diversity is represented by sequenced genomes. Here, we present 824 actinobacterial isolate genomes in the context phylum-wide analysis 6,700 including public isolates metagenome-assembled (MAGs). We estimate that 30%–50% projected phylogenetic possesses genomic representation...
Three strains of Gram-negative, aerobic, neutrophilic and halophilic bacteria were isolated from samples a salt lake on the Qinghai–Tibet Plateau subterranean saline well in Si-Chuan Basin China. These isolates, designated AJ275 T , AJ282 ZG16 investigated using polyphasic approach. Based 16S rRNA gene sequence analysis, isolates could be affiliated to genus Halomonas . Genomic DNA G+C contents 65.9 mol% for 56.7 57.6 The results DNA–DNA hybridizations, fatty acid analysis physiological...
The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project was launched by the JGI in 2007 as a pilot to sequence about 250 bacterial archaeal genomes elevated phylogenetic diversity. Herein, we propose extend this approach type strains prokaryotes associated with soil or plants their close relatives well from newly described species. Understanding microbiology is critical many DOE mission areas, such biofuel production biomass, biogeochemistry, carbon cycling. We are also targeting...
Numerous mountain glaciers located on the Tibetan Plateau are inhabited by abundant microorganisms. The microorganisms glacier surface exposed to cold, barren and high-ultraviolet radiation environments. Although microbial community composition has been revealed high-throughput sequencing, little is known about microevolution adaptive strategy of certain bacterial populations. In this study, we used a polyphasic approach determine taxonomic status 11 psychrophilic Flavobacterium strains...
The World Data Centre for Microorganisms (WDCM) was established 50 years ago as the data center of Federation Culture Collections (WFCC)-Microbial Resource Center (MIRCEN). WDCM aims to provide integrated information services using big technology microbial resource centers and microbiologists all over world. Here, we an overview including its services. Information Worldwide (CCINFO) provides metadata on 708 culture collections from 72 countries regions. Global Catalogue Microorganism (GCM)...
Strain B528-3 T , a Gram-stain-negative, rod-shaped, aerobic, facultatively psychrophilic bacterium with polar flagella, was isolated from an ice core drilled Muztagh Glacier, Xinjiang, China. The novel isolate classified into the genus Massilia . 16S rRNA gene sequence of shares pairwise similarity less than 97 % those all type strains major fatty acids strain were summed feature 3 (C 16:1 ω7 c and/or iso-C 15:0 2-OH) (57.31 %), C 16:0 (11.46 %) and 18:1 (14.72 %). predominant isoprenoid...
Talaromyces contains both asexual and sexually reproducing species. This genus is divided in seven sections currently has 105 accepted In this study we investigated the isolates that were obtained during a of indoor air collected Beijing, China. These strains are resolved four sections, them identified as T. islandicus, aurantiacus, siamensis albobiverticillius according to BenA sequences, while 14 have divergent sequences described here nine new The species placed namely Helici, Islandici,...
Talaromyces is a monophyletic genus containing seven sections. The number of species in grows rapidly due to reliable and complete sequence data contributed from all over the world. In this study agricultural soil samples Fujiang, Guangdong, Jiangxi, Shandong, Tibet Zhejiang provinces China were collected analyzed for fungal diversity. Based on polyphasic approach including phylogenetic analysis partial ITS, BenA, CaM RPB2 gene sequences, macro- micro-morphological analyses, six them could...
An aerobic, motile, Gram-positive strain, DX3-12 T , was isolated from coastal sediment of the Eastern China Sea in Fujian Province, China, and identified by means polyphasic taxonomy. On basis 16S rRNA gene sequence similarity, strain closely related to members genera Planomicrobium Planococcus . similarities between mcmeekinii koreense okeanokoites psychrophilus alkanoclasticus were 97·8, 97·1, 97·5 96·4 %, respectively. Chemotaxonomic physiological properties supported its affiliation...
Gliocladinins A (1) and B (2), two new p-terphenyl derivatives, have been isolated from solid cultures of an isolate Gliocladium sp. that colonizes Cordyceps sinensis. The structures these compounds were determined mainly by analysis their NMR spectroscopic data. In standard disk assays, 1 2 showed modest antimicrobial activity against Staphylococcus aureus (ATCC 6538). addition, displayed inhibitory effects on the growth human tumor cell lines, HeLa HCT116.
A Gram-stain-negative, heterotrophic, aerobic, non-spore-forming and non-motile bacterial strain, designated LM5 T , was isolated from activated sludge a sequencing batch reactor for the treatment of effluents contaminated by malachite green. The taxonomy strain studied phenotypic phylogenetic methods. Strain formed orange colonies on R2A YP plates. Cells were rods, 0.4–0.6 μm in diameter 0.8–1.2 length. Growth occurred at 10–35 °C (optimum, 20–25 °C), pH 5.5–9.5 6.5–7.5) presence 0–2 %...
Two extremely halophilic archaea, strains RO2-11(T) and HO2-1, were isolated from two Chinese marine solar salterns, Rudong saltern Haimen saltern, respectively. Cells of the polymorphic Gram-stain-negative; colonies red-pigmented. The grew at NaCl concentrations 2.6-4.3 M (optimum 3.9 M) required least 0.1 Mg2+ for growth. They able to grow over a pH range 6.0-8.0 temperature 20-50 degrees C, with optimal 7.5 37 C. major polar lipids strain HO2-1 phosphatidylglycerol, phosphatidylglycerol...
Two psychrophilic strains, Cr7-05(T) and Cr4-44(T), isolated from alpine glacier cryoconite, were characterized by using a polyphasic approach. Both strains psychrophilic, showing good growth over temperature range of 1-20 °C. The chemotaxonomic characteristics these isolates included the presence C(18:1)ω7c summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c) as major cellular fatty acids, Q-10 predominant ubiquinone diphosphatidylglycerol, phosphatidylglycerol unknown glycolipids polar lipids....
A Gram-positive, non-motile, rod-shaped, psychrophilic actinomycete, designated strain Cr7-14 T , was isolated from alpine glacier cryoconite. Phylogenetic analysis based on 16S rRNA gene sequences showed that related to members of the genus Nocardioides and shared highest sequence similarities with type strains furvisabuli (98.6 %), ganghwensis (98.2 oleivorans (98.1 %) exalbidus (97.6 %). The predominant cellular fatty acids were C 17 : 1 ω8 c (39.5 iso-C 16 0 (32.4 major menaquinone...
Throughout the long history of industrial and academic research, many microbes have been isolated, characterized preserved (whenever possible) in culture collections. With steady accumulation observational data biodiversity as well microbial sequencing data, bio-resource centers to function information repositories serve academia, industry, regulators on behalf for general public. Hence, World Data Centre Microorganisms (WDCM) started take its responsibility constructing an effective...
Genomic information is essential for taxonomic, phylogenetic, and functional studies to comprehensively decipher the characteristics of microorganisms, explore microbiomes through metagenomics, answer fundamental questions nature human life. However, large gaps remain in available genomic sequencing published bacterial archaeal species, are even larger fungal type strains. The Global Catalogue Microorganisms (GCM) leads an internationally coordinated effort sequence strains close maps...
AbstractFarnesol (FOH) is known to induce apoptosis in some fungi and mammalian cells. We treated Aspergillus flavus, one of the leading causes human invasive aspergillosis a key producer most potent naturally occurring hepatocarcinogenic compounds, with FOH assess its effect on viability fungus. strongly inhibited germination growth A. flavus induced markers for including nuclear condensation, phosphatidylserine (PS) externalization, DNA fragmentation intracellular reactive oxygen species...
Five aerobic, Gram-stain-negative bacterial strains, designated as C3-2-a3 T , B3-2-R+30, C3-2-a4, C3-2-M3 and C3-2-M8, were isolated from the coastal soil of LungmuCo Lake in Tibet Autonomous Region, PR China. Phylogenetic analyses based on 16S rRNA genes genomes indicated that these isolates belonged to genus Luteimonas showed a high similarity suaedae LNNU 24178 (99.01%), endophytica RD2P54 (98.80%) salinisoli SJ-92 (97.67%). The average nucleotide identity (ANI) digital DNA–DNA...