A5042136634- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Plant Pathogens and Fungal Diseases
- Microbial Community Ecology and Physiology
- Plant Parasitism and Resistance
- Bacterial Genetics and Biotechnology
- Plant tissue culture and regeneration
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Bacterial biofilms and quorum sensing
- Bacillus and Francisella bacterial research
- Transgenic Plants and Applications
- Protist diversity and phylogeny
- Research in Cotton Cultivation
- Metalloenzymes and iron-sulfur proteins
- Inflammasome and immune disorders
- Pain Mechanisms and Treatments
- Phosphodiesterase function and regulation
- Antioxidants, Aging, Portulaca oleracea
- Heat Transfer Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Fern and Epiphyte Biology
Institute of Agricultural Resources and Regional Planning
2017-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Chinese Academy of Agricultural Sciences
2012-2025
Zhengzhou University
2005-2024
University of Saskatchewan
2024
Plant (United States)
2012-2019
Cornell University
2012-2019
Chinese Academy of Sciences
2006-2016
Institute of Microbiology
2006-2016
Kunming Institute of Botany
2008-2009
Microbiomes are important for crop performance. However, a deeper knowledge of crop-associated microbial communities is needed to harness beneficial host-microbe interactions. Here, by assessing the assembly and functions maize microbiomes across soil types, climate zones, genotypes, we found that stem xylem selectively recruits highly conserved microbes dominated Gammaproteobacteria. We showed proportion bacterial taxa carrying nitrogenase gene (nifH) was larger in than other organs such as...
Plants and their associated microbiota constitute an assemblage of species known as holobionts. The plant seed microbiome plays important role in nutrient uptake stress attenuation. However, the core vertically transmitted endophytes remain largely unexplored.
The effects of phosphate-solubilizing bacteria (PSB) on plant productivity are high variable under field conditions. Soil phosphorus (P) levels proposed to impact PSB performance. Furthermore, the effect exogenous rhizosphere microbial community and their functions largely unexplored. Our study examined how different P background fertilization affected performance Pseudomonas asiatica JP233. We further conducted metagenomic sequencing assess its microbiota functions, with a focus genes...
The bacterial pathogen Pseudomonas syringae pv. tomato DC3000 suppresses the two-tiered innate immune system of Nicotiana benthamiana and other plants by injecting a complex repertoire type III secretion effector (T3E) proteins. Effectorless polymutant DC3000D36E was used with modularized for native delivery 29 T3Es singly in pairs. Assays performance this T3E library N. leaves revealed matrix interplay, six eliciting death eight others variously suppressing activity six. also interrogated...
The Microbiome Protocols eBook (MPB) serves as a crucial bridge, filling gaps in microbiome protocols for both wet experiments and data analysis. first edition, launched 2020, featured 152 meticulously curated protocols, garnering widespread acclaim. We now extend sincere invitation to researchers participate the upcoming 2nd version of MPB, contributing their valuable advance research. is research area that focuses on studying omics microbe [1] with rapid development past few decades,...
The manipulation of cytokinin contents via Agrobacterium-mediated transformation is an efficient tool for delaying leaf senescence and improving the resistance to environmental stresses. In present study, cotton transformants harbouring Agrobacterium tumefaciens isopentenyl transferase (IPT) gene under control promoter Gossypium hirsutum cysteine proteinase (Ghcysp) were generated. PCR Southern blot analysis indicated that foreign DNA fragment was successfully integrated into genome....
The type III secretion system (T3SS) is required for virulence in the gram-negative plant pathogen Pseudomonas syringae pv. tomato DC3000. alternative sigma factor HrpL directly regulates expression of T3SS genes via a promoter sequence, often designated as “hrp promoter.” Although regulon has been extensively investigated DC3000, it not known whether additional members remain to be found. To systematically search HrpL-regulated genes, we used chromatin immunoprecipitation coupled with...
Talaromyces is a monophyletic genus containing seven sections. The number of species in grows rapidly due to reliable and complete sequence data contributed from all over the world. In this study agricultural soil samples Fujiang, Guangdong, Jiangxi, Shandong, Tibet Zhejiang provinces China were collected analyzed for fungal diversity. Based on polyphasic approach including phylogenetic analysis partial ITS, BenA, CaM RPB2 gene sequences, macro- micro-morphological analyses, six them could...
Highlights•SLT domain and catalytic glutamate in HrpH are essential for effector translocation•HrpH SLT elicit PCD inhibit PTI, regardless of glutamate•The is dispensable the interactions between ATL2•ATL2 HrpH-induced inhibition MAPK SA signalingSummaryPseudomonas syringae deploys a type III secretion system (T3SS) to deliver proteins facilitate infection plant cells; however, little known about direct T3SS components plants. Here, we show that specialized lytic transglycosylase (SLT) P....
Introduction Plants detect the invasion of microbial pathogens through pathogen-associated molecular patterns (PAMPs). Cold shock proteins (CSPs) are a class PAMPs specifically recognized by Solanales plants. While peptide inoculation studies have revealed effects CSPs, their in vivo roles remain poorly understood. Methods A model system involving interactions between Pseudomonas fluorescens and P. syringae pv. tomato DC3000 with Nicotiana benthamiana has been widely used to investigate...
Many bacterial pathogens inject a cocktail of effector proteins into host cells through type III secretion systems. These effectors act in concert to modulate physiology and immune signaling, thereby promoting pathogenicity. In search for additional Pseudomonas syringae suppressing plant innate immunity triggered by pathogen or microbe-associated molecular patterns (PAMPs MAMPs), we identified P. tomato DC3000 HopF2 as potent suppressor early immune-response gene transcription...
Flagella power bacterial movement through liquids and over surfaces to access or avoid certain environmental conditions, ultimately increasing a cell's probability of survival reproduction. In some cases, flagella chemotaxis are key virulence factors enabling pathogens gain entry attach suitable host tissues. However, not always beneficial; both plant animal immune systems have evolved receptors sense the proteins that make up flagellar filaments as signatures infection. Microbes poorly...
Abstract Strain S150 was isolated from the tobacco rhizosphere as a plant growth‐promoting rhizobacterium. It increased fresh weight significantly and lateral root development, it antagonized pathogenic fungi but not phytobacteria. Further tests showed that strain solubilized organic phosphate produced ammonia, siderophore, protease, amylase, cellulase, did produce indole‐3‐acetic acid. Using morphology, physiological characteristics, multi‐locus sequence analysis, identified Pseudomonas...
Bacterial flagellin is perceived as a microbe (or pathogen)-associated molecular pattern (MAMP or PAMP) by the extracellular recognition receptors, FLS2 and TLR5, of plants mammals respectively. Flagellin accidently translocated into mammalian cells pathogen type III secretion systems (T3SSs) recognized nucleotide-binding leucine-rich repeat receptor NLRC4 pathogenesis induces death-associated immune response. The non-pathogen Pseudomonas fluorescens Pf0-1, expressing syringae T3SS, plant P....
Flagellins are the main constituents of flagellar filaments that provide bacterial motility, chemotactic ability, and host immune elicitation ability. Although functions flagellins have been extensively studied in bacteria with a single flagellin-encoding gene, function multiple genes species is largely unknown. Here, model plant-growth-promoting bacterium Pseudomonas kilonensis F113 was used to decipher divergent duplicated flagellins. We demonstrate two (FliC-1 FliC-2) 12 strains,...
The multifunctional secondary metabolites known as cyclic lipopeptides (CLPs), which are produced by a large variety of bacteria, have become key category plant immunity elicitors. Pseudomonas-CLPs (Ps-CLPs) extremely diverse in structure and biological activity. However, an understanding CLP-plant structure–function interactions currently remains elusive. Here, we identify medpeptin, novel CLP from Pseudomonas mediterranea that consists 22 amino acids. Medpeptin is synthesized non-ribosomal...
This study investigated the change in microbiome of tomato rhizosphere soils after invasion