MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are short (19–25 nucleotides) non-coding RNA molecules that have large-scale regulatory effects on development stress responses in plants. Verticillium wilt is a vascular disease plants caused by the fungal pathogen dahliae. The objective of this study to investigate transcriptional profile miRNAs other Verticillium–inoculated cotton roots. Four libraries were constructed from mocked infected roots two cultured species which with...

Superoxide dismutases (SODs) are a key antioxidant enzyme family, which have been implicated in protecting plants against the toxic effects of reactive oxygen species. Despite current studies shown that gene family involved plant growth and developmental processes biotic abiotic stress responses, little is known about its functional role upland cotton. In present study, we comprehensively analyzed characteristics SOD cotton (Gossypium hirsutum). Based on their conserved motifs, 18 GhSOD...

Long non-coding (lnc) RNAs are a class of functional RNA molecules greater than 200 nucleotides in length, and lncRNAs play important roles various biological regulatory processes response to the biotic abiotic stresses. LncRNAs associated with salt stress cotton have been identified through sequencing, but function has not reported. We previously stress-related (Gossypium spp.), discovered salt-related lncRNA-lncRNA973.In this study, we expression level, localization, function, preliminary...

Long non-coding RNAs (lncRNAs) play important roles in response to biotic and abiotic stress through acting as competing endogenous (ceRNAs) decoy mature miRNAs. However, whether this mechanism is involved cotton salt remains unknown. We report the characterization of an lncRNA, lncRNA354, whose expression was reduced salt-treated localized at nucleus cytoplasm. Using target mimic (eTM) analysis, we predicted that lncRNA354 had a potential binding site for miR160b. Transient tobacco...

Catalases (CATs), which were coded by the catalase gene family, a type notably distinguished ROS-metabolizing proteins implicated to perform various physiological functions in plant growth, development and stress responses. However, no systematical study has been performed cotton. In present study, we identified 7 CAT genes genome of Gossypium hirsutum L. Additionally, G. barbadense L., respectively. The results phylogenetic synteny analysis showed that divided into two groups, whole-genome...

The data presented in this paper is supporting the research article "Genome-Wide Analysis of Superoxide Dismutase Gene Family Gossypium raimondii and G. arboreum" [1]. In article, we present phylogenetic tree showing dichotomy with two different clusters SODs inferred by Bayesian method MrBayes (version 3.2.4), "Bayesian inference under mixed models" [2], Ramachandran plots arboreum SODs, protein sequence used to generate 3D sructure proteins template accession via SWISS-MODEL server,...

Salinity stress is a major abiotic affecting the productivity and fiber quality of cotton. Although reactive oxygen species (ROS) play critical roles in plant responses, their complex molecular regulatory mechanism under salinity largely unknown cotton, especially microRNA (miRNA)-mediated regulation superoxide dismutase gene expression. Here, we report that cotton iron GhFSD1 miRNA ghr-miR414c work together response to stress. The targets coding sequence region GhFSD1, inhibiting expression...

The manipulation of cytokinin contents via Agrobacterium-mediated transformation is an efficient tool for delaying leaf senescence and improving the resistance to environmental stresses. In present study, cotton transformants harbouring Agrobacterium tumefaciens isopentenyl transferase (IPT) gene under control promoter Gossypium hirsutum cysteine proteinase (Ghcysp) were generated. PCR Southern blot analysis indicated that foreign DNA fragment was successfully integrated into genome....

MicroRNAs (miRNAs) are a group of endogenous small non-coding RNAs that play important roles in plant growth, development, and stress response processes. Verticillium wilt is vascular disease plants mainly caused by dahliae Kleb., the soil-borne fungal pathogen. However, role miRNAs regulation defense responses mostly unknown. This study aimed to identify new their potential targets involved responses. Four RNA libraries two degradome from mock-infected infected roots cotton (both Gossypium...

Superoxide dismutases (SODs) convert highly reactive superoxide radicals to hydrogen peroxide and molecular oxygen, belong a class of proteins with important roles in plant responses stress. Genome-wide analysis was performed cotton species Gossypium raimondii arboreum characterize SOD genes proteins. From the two genomes, 18 were identified several bioinformatics tools, classified into subfamilies: Cu/Zn-SODs (ten genes) Mn/Fe-SODs (eight genes). The highest number on chromosome 13 G....

MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation.A large number of miRNAs have been identified from model plant species; however, the character conserved is poorly understood.We studied 42 miRNA families within kingdom, using miRBase database.Some were found to be preferentially expressed in dicots relative monocots, especially miR403, miR472 and miR479.Using an improved homology search-based approach as query...

Soil salinity is a major environmental stress limiting plant growth and productivity. We have reported previously the isolation of an Apocynum venetum DEAD-box helicase 1 (AvDH1) that expressed in response to salt exposure. Here, we report overexpression AvDH1 driven by constitutive cauliflower mosaic virus-35S promoter cotton plants confers tolerance. Southern Northern blotting analyses showed gene was integrated into genome expressed. In this study, transgenic expressing evaluated under...

Abstract Background Plant NADPH oxidase (NOX), also known as respiratory burst homolog (rboh), encoded by the rboh gene, is a key enzyme in reactive oxygen species (ROS) metabolic network. It catalyzes formation of superoxide anion (O 2 •− ), type ROS. In recent years, various studies had shown that members plant gene family were involved growth and developmental processes well biotic abiotic stress responses, but little about its functional role upland cotton. Results present study, 26...

The genes encoding DEAD-box helicases play a key role in various abiotic stresses, including temperature, light, oxygen, and salt stress. A salt-responsive gene, designated AvDH1, was isolated from the halophyte dogbane (Apocynum venetum) by using suppression subtractive hybridization RACE (rapid amplification of cDNA ends) PCR. deduced amino acid sequence has nine conserved helicase motifs protein family. AvDH1 gene is present as single copy genome. This expressed response to NaCl not...

The RNA helicases, which help to unwind stable duplexes, and have important roles in metabolism, belong a class of motor proteins that play plant development responses stress. Although this family genes has been the subject systematic investigation Arabidopsis, rice, tomato, it not yet characterized cotton. In study, we identified 161 putative helicase genome diploid cotton species Gossypium raimondii. We classified these into three subfamilies, based on presence either DEAD-box (51 genes),...

Drought stress markedly constrains plant growth and diminishes crop productivity. Strigolactones (SLs) exert a beneficial influence on resilience to drought conditions. Nevertheless, the specific function of SLs in modulating cotton's response remains be elucidated. In this study, we assess impact exogenous SL (rac-GR24) administration at various concentrations (0, 1, 5, 10, 20 µM) cotton during stress. The findings reveal that seedlings treated with 5 µM exhibit optimal mitigation...

The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage its native host cerana.Understanding resistance mechanisms of eastern bees against mites will help researchers determine how protect other species from this organism.The A. cerana genome not been previously sequenced; hence, here we sequenced nurse workers transcriptome and monitored differential gene expression challenged by V. destructor.Using de novo assembly,...