A5003419454- Epigenetics and DNA Methylation
- Endometrial and Cervical Cancer Treatments
- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- PI3K/AKT/mTOR signaling in cancer
- Ovarian cancer diagnosis and treatment
- Cytokine Signaling Pathways and Interactions
- Cancer-related gene regulation
- Diet, Metabolism, and Disease
- Telomeres, Telomerase, and Senescence
- Gynecological conditions and treatments
- Prostate Cancer Treatment and Research
- Cancer-related Molecular Pathways
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Lymphoma Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Prostate Cancer Diagnosis and Treatment
- Hormonal and reproductive studies
- Uterine Myomas and Treatments
- Hypothalamic control of reproductive hormones
- Diet and metabolism studies
Universität Innsbruck
2022-2025
Tirol Kliniken
2024
Medical University of Vienna
2016-2023
Cervical cancer (CC) screening in women comprises human papillomavirus (HPV) testing followed by cytology triage of positive cases. Drawbacks, including cytology's low reproducibility and requirement for short intervals, raise the need alternative methods. Here we used an innovative technique, WID-qCIN test, to assess DNA methylation genes DPP6, RALYL GSX1 a real-life cohort 28,017 aged ≥30 years who attended CC Stockholm between January March 2017. In analysis all 2,377 HPV-positive...
Endometrial cancer (EC) incidence has been rising over the past 10 years. Delays in diagnosis reduce survival and necessitate more aggressive treatment. We aimed to develop validate a simple, noninvasive, reliable triage test for EC number of invasive diagnostic procedures improve patient survival.We developed screen women with suspected using 726 cervical smear samples from without EC, validated 562 cervicovaginal three different collection methods (cervical smear: n = 248; vaginal swab:...
Abstract Prostate cancer (PCa) is a common and fatal type of in men. Metastatic PCa (mPCa) major factor contributing to its lethality, although the mechanisms remain poorly understood. PTEN one most frequently deleted genes mPCa. Here we show frequent genomic co-deletion STAT3 liquid biopsies patients with Loss Stat3 Pten -null mouse prostate model leads reduction LKB1/pAMPK simultaneous activation mTOR/CREB, resulting metastatic disease. However, constitutive led high levels suppressed...
Aberrant DNA methylation patterns in malignant cells allow insight into tumor evolution and development can be used for disease classification. Here, we describe the genome-wide signatures of NPM-ALK-positive (ALK+) NPM-ALK-negative (ALK-) anaplastic large-cell lymphoma (ALCL). We find that ALK+ ALK- ALCL share common changes genes involved T cell differentiation immune response, including TCR CTLA-4, without an ALK-specific impact on gene promoters. Furthermore, uncover a close relationship...
BackgroundTo detect uterine cancer, simpler and more specific index tests are needed to triage women with abnormal bleeding a reference histology test. We aimed compare the performance of conventional imaging novel WID-qEC DNA methylation test in terms detecting presence or absence cancers bleeding.MethodsEPI-SURE was prospective, observational study that invited all aged 45 years older attending tertiary gynaecological diagnostic referral centre at University College London Hospital...
Aging is a key risk factor for many diseases, including cancer, and better understanding of its underlying molecular mechanisms may help to prevent, delay, or treat age-related pathologies. Epigenetic alterations such as DNA methylation (DNAme) changes are hallmark aging form the basis so-called epigenetic clocks, yet their functional relevance directionality in different organs during disease development often unclear. Here, we link cell-specific DNAme with three hallmarks cancer...
Abstract Endometrial cancer (EC) is the most prevalent gynaecological in high‐income countries and its incidence continuing to rise sharply. Simple objective tools reliably detect women with EC are urgently needed. We recently developed validated DNA methylation (DNAme)‐based women's risk identification—quantitative polymerase chain reaction test for endometrial (WID‐qEC) that could address this need. Here, we demonstrate stability of WID‐qEC remains consistent regardless of: (i)...
The majority of endometrial and cervical cancers present with abnormal vaginal bleeding but only a small proportion women suffering from actually have such cancer. A simple, operator-independent accurate test to correctly identify presenting as consequence or cancer is urgently required. We recently developed validated the WID-qEC test, which assesses DNA methylation ZSCAN12 GYPC via real-time PCR, triage symptoms suggestive using ThinPrep-based liquid cytology samples. Here, we investigated...
Abstract Background Cervical screening using primary human papilloma virus (HPV) testing and cytology is being implemented in several countries. Cytology as triage for colposcopy referral suffers from shortcomings. HPV overcomes some of these but lacks specificity women under 30. Here, we aimed to develop validate an automatable test that highly sensitive specific independently age sample heterogeneity, predicts progression CIN3+ HPV+ patients. Results The WID™-qCIN, assessing three regions...
Quantification of the antiprogestin mifepristone and its active metabolite metapristone in plasma tissue female mice using HPLC-MS/MS.
Abstract The Illumina Methylation array platform has facilitated countless epigenetic studies on DNA methylation (DNAme) in health and disease, yet relatively few have so studied its reliability, i.e., the consistency of repeated measures. Here we investigate reliability both type I II Infinium probes. We propose a method for excluding unreliable probes based dynamic thresholds mean intensity (MI) ‘unreliability’, estimated by probe-level simulation influence technical noise β values using...
Malignant transformation depends on genetic and epigenetic events that result in a burst of deregulated gene expression chromatin changes. To dissect the sequence this process, we used T-cell–specific lymphoma model based human oncogenic nucleophosmin-anaplastic kinase (NPM-ALK) translocation. We find T cells shifts thymic cell populations to an undifferentiated immunophenotype, which occurs only after period latency, accompanied by induction MYC-NOTCH1 axis deregulation key enzymes....
Abstract Endometrial cancer incidence has been rising over the past ten years. Delays in diagnosis reduce survival and necessitate more aggressive treatment. We developed WID-qEC test, a PCR-based test that evaluates DNA methylation gene regions of GYPC ZSCAN12, using 726 cervico-vaginal samples from women with without endometrial cancer. So far, validated an additional 1388 various settings, including case/control study utilizing self-samples nested set screening cohort taken up to 3 years...
<h3>Introduction/Background</h3> Endometrial cancer incidence is rising. Delays in diagnosis reduce survival and necessitate more aggressive treatment. We developed the qPCR-based WID-qEC test that evaluates DNA methylation GYPC ZSCAN12 genes for detecting uterine cancer. In a prospective, observational study, we compared performance of conventional index imaging endometrial detection symptomatic women. <h3>Methodology</h3> invited all women aged ≥ 45 years with abnormal bleeding attending...
The burden of uterine cancer is growing and, in the US and UK, mortality rates are poorest among black women. Early detection these cancers critical poor performance ultrasound women may contribute to adverse outcomes. Limited data on this topic available from Africa. We assessed whether a simple DNA methylation test, WID-qEC, enables all epithelial (endometrial cervical) presenting with abnormal bleeding (AUB) Ghana. Among 118 ≥40 years AUB, 106 consented study cervicovaginal sample was...
ABSTRACT The Illumina Methylation array platform has facilitated countless epigenetic studies on DNA methylation (DNAme) in health and disease, yet relatively few have so studied its reliability, i.e., the consistency of repeated measures. Here we focus reliability both type I II Infinium probes. We propose a method for excluding unreliable probes based dynamic thresholds mean intensity (MI) ‘unreliability’, estimated by probe-level simulation influence technical noise β-values using...
ABSTRACT Malignant transformation depends on genetic and epigenetic events that result in a burst of deregulated gene expression chromatin changes. To dissect the sequence this process, we used T cell-specific lymphoma model based human oncogenic NPM-ALK translocation. We find cells shifts thymic cell populations to an undifferentiated immunophenotype, which occurs only after period latency, accompanied by induction MYC-NOTCH1 axis deregulation key enzymes. discover aberrant DNA methylation...
Abstract Prostate cancer (PCa) lethality is driven by its progression to a metastatic castration-resistant state, yet the signaling mechanisms underlying spread remain unknown. Here we show that STAT3 converges with LKB1/mTORC1 and CREB control disease in PCa mouse models. Unexpectedly, was found be upregulated diabetic patients undergoing metformin therapy concomitant reduction mTORC1 expression. In preclinical models of PCa, genetic ablation or activation had opposing effects on...