A5068835449- Retinal Development and Disorders
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- Neuroscience and Neural Engineering
- Retinal Diseases and Treatments
- Receptor Mechanisms and Signaling
- Ophthalmology and Visual Impairment Studies
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Glaucoma and retinal disorders
- Corneal surgery and disorders
- Retinopathy of Prematurity Studies
- Adenosine and Purinergic Signaling
- Neural dynamics and brain function
- Connexins and lens biology
- Nicotinic Acetylcholine Receptors Study
- melanin and skin pigmentation
- Visual perception and processing mechanisms
- Diabetes Treatment and Management
- Sleep and related disorders
- Neuroscience and Music Perception
- Spaceflight effects on biology
- Pharmacological Receptor Mechanisms and Effects
- Neuropeptides and Animal Physiology
State Key Laboratory of Medical Neurobiology
2015-2024
Fudan University
2015-2024
Eye & ENT Hospital of Fudan University
2023
Zhongshan Hospital
2018-2022
Shanghai Medical College of Fudan University
2021-2022
Huashan Hospital
2019-2022
The First Affiliated Hospital, Sun Yat-sen University
2019-2021
Sun Yat-sen University
2019-2021
Brown University
2008-2017
John Brown University
2017
The photopigment melanopsin confers photosensitivity upon a minority of retinal output neurons. These intrinsically photosensitive ganglion cells (ipRGCs) are more diverse than once believed, comprising five morphologically distinct types, M1 through M5. Here, in mouse retina, we provide the first in-depth characterization M4 cells, including their structure, function, and central projections. apparently correspond to ON α earlier reports, easily distinguished from other ipRGCs by very large...
Using multi-color visible lights for independent optogenetic manipulation of multiple neuronal populations offers the ability sophisticated brain functions and behavior dissection. To mitigate invasive fiber insertion, infrared light excitable upconversion nanoparticles (UCNPs) with deep tissue penetration have been implemented in optogenetics. However, due to chromatic crosstalk induced by emission peaks, conventional UCNPs or their mixture cannot independently activate targeted...
The increasing global prevalence of myopia calls for elaboration the pathogenesis this disease. Here, we show that selective ablation and activation intrinsically photosensitive retinal ganglion cells (ipRGCs) in developing mice induced myopic hyperopic refractive shifts by modulating corneal radius curvature (CRC) axial length (AL) an opposite way. Melanopsin- rod/cone-driven signals ipRGCs were found to influence development affecting AL CRC, respectively. role progression is evidenced...
We identified the ON-OFF direction-selective ganglion cells (DSGCs) in mouse retina and characterized their physiological, morphological pharmacological properties. These showed transient responses to onset termination of a stationary flashing spot, strong directional selectivity moving rectangle. Application various reagents demonstrated that DSGCs utilize similar array transmitters receptors compute motion direction counterparts rabbit retina. Voltage clamp recording receive larger...
Intrinsically photosensitive retinal ganglion cells (iprgcs) are depolarized by light two mechanisms: directly, through activation of their photopigment melanopsin; and indirectly synaptic circuits driven rods cones. To learn more about the rod cone driving ipRGCs, we made multielectrode array (MEA) patch-clamp recordings in wildtype genetically modified mice. Rod-driven ON inputs to ipRGCs proved be as sensitive any reaching conventional cells. These signals presumably pass part primary...
The visual system consists of two major subsystems, image-forming circuits that drive conscious vision and non-image-forming for behaviors such as circadian photoentrainment. While historically considered non-overlapping, recent evidence has uncovered crosstalk between these subsystems. Here, we investigated shared developmental mechanisms. We revealed an unprecedented role light in the maturation clock discovered intrinsically photosensitive retinal ganglion cells (ipRGCs) are critical this...
Light modulates mood through various retina-brain pathways. We showed that mice treated with short-term acute bright light exposure displayed anxiety-related phenotypes in a prolonged manner even after the termination of exposure. Such postexposure anxiogenic effect depended upon melanopsin-based intrinsically photosensitive retinal ganglion cell (ipRGC) activities rather than rod/cone photoreceptor inputs. Chemogenetic manipulation specific central nuclei demonstrated ipRGC-central amygdala...
Purpose.: Retinal dopamine has been long implicated in the signaling pathway regulating eye growth, as evidenced by its reduced levels myopic eyes various species. We examined whether and how retinal were changed a C57BL/6 mouse model of experimental myopia. Methods.: Form-deprivation myopia (FDM) was induced mice wearing monocular occluder for 4 weeks. Refractive errors measured using an infrared photorefractor. dopamine/DOPAC vitreal DOPAC assessed high-performance liquid chromatography...
Abstract We previously demonstrated that for long-term spastic limb paralysis, transferring the seventh cervical nerve (C7) from nonparalyzed side to paralyzed results in increase of 17.7 Fugl-Meyer score. One strategy further improvement voluntary arm movement is selective activation five target muscles innervated by C7 during recovery process. In this study, we develop an implantable multisite optogenetic stimulation device (MOSD) based on shape-memory polymer. Two-site sciatic bundles...
Purpose: To investigate whether choroidal vascularity participates in high-dose atropine's antimyopia and rebound mechanisms. Methods: A mediation analysis was embedded within a randomized controlled trial. In total, 207 myopic children were assigned randomly to group A/B. Participants received 1% atropine weekly (phase 1) 0.01% daily 2) for 6 months each. Those B 1 year. Four plausible intervention mediators assessed: total area (TCA), luminal (LA), stromal (SA), index (CVI). Results: A,...
Intrinsically photosensitive retinal ganglion cells (ipRGCs) project to the suprachiasmatic nucleus (SCN) and are essential for normal photic entrainment of global circadian rhythms in physiology behavior. The effect light on central clock is dependent phase, retina itself contains intrinsic oscillators that can alter its sensitivity light. This raises possibility ipRGCs, hence photoentraining signals retinohypothalamic tract, subject modulation. Although ipRGC photopigment melanopsin...
Green light exposure has been shown to reduce pain in animal models. Here, we report a vision-associated enkephalinergic neural circuit responsible for green light–mediated analgesia. Full-field at an intensity of 10 lux produced analgesic effects healthy mice and model arthrosis. Ablation cone photoreceptors completely inhibited the effect, whereas rod ablation only partially reduced relief. The effect was not modulated by intrinsically photosensitive retinal ganglion cells (ipRGCs), which...
Selective responses of retinal ganglion cells (RGCs) to the direction motion have been recorded extracellularly from rabbit and mouse retina at eye opening. Recently, it has shown that development this circuitry is light independent. Using whole-cell patch clamp recording, we report here early postnatal direction-selective (DSGCs) showed lower membrane excitability, reliability synaptic transmission much slower kinetics compared with adult DSGCs. However, degree selectivity DSGCs measured by...
Although retinal dopamine (DA) has been long implicated in myopia development, current studies demonstrate that DA levels are unaltered C57BL/6 mice with form-deprivation myopia. This work was undertaken to explore whether and how refractive development is perturbed this mouse strain when reduced by 6-hydroxydopamine (6-OHDA) administration.On two successive days, 6-OHDA injected into the vitreous of P18 mice. Retinal were measured HPLC TH analyzed quantitative Western blotting. To choose...
Significant progress has been made recently in treating neurological blindness using implantable visual prostheses. However, medical devices are highly invasive and subject to many safety, efficacy, cost issues. The discovery that ultrasound (US) may be useful as a noninvasive neuromodulation tool aroused great interest the field of acoustic retinal prostheses (ARPs). We have investigated responsiveness rat ganglion cells (RGCs) low-frequency focused US stimulation (LFUS) at 2.25 MHz...
We investigate morphologic and physiologic alterations of ganglion cells (GCs) in a streptozocin (STZ)-induced diabetic mouse model.Experiments were conducted flat-mount retinas mice 3 months after the induction diabetes. Changes morphology four subtypes GCs (ON-type RGA2 [ON-RGA2], OFF-type [OFF-RGA2], ON-type RGC1 [ON-RGC1], ON-OFF type RGD2 [ON-OFF RGD2]) characterized Thy1-YFP transgenic mice. Using whole-cell patch-clamp recording, passive membrane properties action potential (AP)...
Abstract The neural basis of fear heights remains largely unknown. In this study, we investigated the response to in male mice and observed characteristic aversive behaviors resembling human height vertigo. We identified visual input as a critical factor mouse reactions heights, while peripheral vestibular was found be nonessential for heights. Unexpectedly, that naïve does not rely on image-forming processing by primary cortex. Instead, subset neurons ventral lateral geniculate nucleus...
Diabetic retinopathy is a prominent cause of blindness in adults, with early retinal ganglion cell (RGC) loss contributing to visual dysfunction or blindness. In the brain, defects y-aminobutyric acid (GABA) synaptic transmission are associated pathophysiological and neurodegenerative disorders, whereas glucagon-like peptide-1 (GLP-1) has demonstrated neuroprotective effects. However, it not yet clear whether diabetes causes alterations inhibitory input RGCs how GLP-1 protects against...
Impairment of visual function has been detected in the early stage diabetes but underlying neural mechanisms involved are largely unknown. Morphological and functional alterations retinal ganglion cells, final output neurons vertebrate retina, thought to be major cause defects direct evidence support this notion is limited. In study we investigated changes cells a type 1-like diabetic mouse model. Our results demonstrated that spontaneous spiking activity ON-type was increased...
Aerobic exercise has been shown to play a crucial role in preventing neurological diseases and improving cognitive function. In the present study, we investigated effect of treadmill training on retinal ganglion cells (RGCs) following optic nerve transection adult rats. We exercised rats for 5 d/week (30 min/d at rate 9 m/min) or placed control static treadmills. After 3 weeks exercise, left each rat was transected. surgery, another week. The percentages surviving RGCs axotomized eyes...