A5014658271- Wound Healing and Treatments
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Angiogenesis and VEGF in Cancer
- Fibroblast Growth Factor Research
- Tendon Structure and Treatment
- Photoacoustic and Ultrasonic Imaging
- Electrospun Nanofibers in Biomedical Applications
- Skin Protection and Aging
- Single-cell and spatial transcriptomics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Optical Coherence Tomography Applications
- Immune cells in cancer
- Retinoids in leukemia and cellular processes
- Silk-based biomaterials and applications
- Dental Education, Practice, Research
- Periodontal Regeneration and Treatments
- Digestive system and related health
- Innovations in Medical Education
- interferon and immune responses
- Virus-based gene therapy research
- Pharmacological Receptor Mechanisms and Effects
- Dermatologic Treatments and Research
- Nanoplatforms for cancer theranostics
University of Illinois Chicago
2010-2024
ETH Zurich
2016-2023
Loyola University Medical Center
2010
Illinois College
2010
Matrix deposition is essential for wound repair, but when excessive, leads to hypertrophic scars and fibrosis. The factors that control matrix in skin wounds have only partially been identified the consequences of alterations mechanical properties are largely unknown. Here, we report how a single diffusible factor, activin A, affects healing process across scales. Bioinformatics analysis fibroblast transcriptome data combined with biochemical histopathological analyses functional vitro...
Wound healing is a well-coordinated process, necessitating efficient formation of new blood vessels. Vascularization defects are therefore major risk factor for chronic, non-healing wounds. The dynamics mammalian tissue revascularization, vessel maturation, and remodeling remain poorly understood due to lack suitable in vivo imaging tools. A label-free large-scale optoacoustic microscopy (LSOM) approach developed rapid, non-invasive, volumetric regeneration over large areas spanning up 50 mm...
We have employed the receptor‐ligand binding technique in an attempt to determine if specific sites (receptors) for serotonin and opiates are present on rat intestinal epithelial cell membranes. A wide variety of ligands opiate receptors bound receptor brain. However, same failed bind a (receptor‐related) manner isolated membranes ileal colonic cells. Additional washing tissue pellet (to remove soluble peptidases), pretreatment with p‐chlorophenylalanine deplete endogenous serotonin),...
During dermal wound repair, hypoxia-driven proliferation results in dense but highly permeable, disorganized microvascular networks, similar to those solid tumors. Concurrently, activated fibroblasts generate an angiopermissive, provisional extracellular matrix (ECM). Unlike cancers, wounds naturally resolve via blood vessel regression and ECM maturation, which are essential for reestablishing tissue homeostasis. Mechanisms guiding resolution poorly understood; one candidate regulator is...
Cancer-associated fibroblasts (CAFs) are key regulators of tumorigenesis and promising targets for next-generation therapies. We discovered that cancer cell-derived activin A reprograms into pro-tumorigenic CAFs. Mechanistically, this occurs via Smad2-mediated transcriptional regulation the formin mDia2, which directly promotes filopodia formation cell migration. mDia2 also induces expression CAF marker genes through prevention p53 nuclear accumulation, resulting in production a matrisome...
Angiogenesis is regulated by signals received receptor tyrosine kinases such as vascular endothelial growth factor receptors. Mammalian Sprouty (Spry) proteins are known to function specifically antagonizing the activation of mitogen-activated protein kinase signaling pathway kinases, a promote angiogenesis. To examine role Spry2 in regulation angiogenesis during wound repair, we used model murine dermal healing. Full-thickness excisional wounds (3 mm) were made on dorsum anesthetized adult...
Abstract Activin has emerged as an important player in different types of cancer, but the underlying mechanisms are largely unknown. We show here that activin overexpression is early event murine and human skin tumorigenesis. This functionally important, since promoted tumorigenesis mice induced by papillomavirus 8 oncogenes. was accompanied depletion epidermal γδ T cells accumulation regulatory cells. Most importantly, increased number macrophages via attraction blood monocytes, which...
Healing wounds and cancers present remarkable cellular molecular parallels, but the specific roles of healing phases are largely unknown. We developed a bioinformatics pipeline to identify genes pathways that define distinct across time-course healing. Their comparison cancer transcriptomes revealed resolution phase wound signature is associated with increased severity in skin enriches for extracellular matrix-related pathways. Comparisons early- late-phase fibroblasts vs cancer-associated...
This study aimed to provide a first nationwide assessment of dental students’ attitudes toward the importance research and its integration into curriculum. For this purpose, American Association for Dental Research National Student Group developed an online survey that was distributed 89 percent U.S. students in May 2012. The consisted twenty–one Likert–type items divided three groups: dentistry, barriers involvement, exposure There were 733 responses (3.9 response rate), including all...
Article27 July 2020Open Access Source DataTransparent process Antagonism of interferon signaling by fibroblast growth factors promotes viral replication Luigi Maddaluno Corresponding Author [email protected] orcid.org/0000-0002-4325-6220 Department Biology, Institute Molecular Health Sciences, ETH Zurich, Switzerland Search for more papers this author Corinne Urwyler Theresa Rauschendorfer Michael Meyer Debora Stefanova Roman Spörri Microbiology, Mateusz Wietecha Luca Ferrarese Diana...
Hyperosmotic stress occurs in several diseases, but its long-term effects are largely unknown. We used sorbitol-treated human fibroblasts 3D culture to study the consequences of hyperosmotic skin. Sorbitol regulated many genes, which help cells cope with condition. The most robustly gene encodes serine protease 35 (PRSS35). Its regulation by was dependent on kinases p38 and JNK transcription factors NFAT5 ATF2. identified different collagens collagen-associated proteins as putative PRSS35...
Large-ScaLe OptOacOuStic MicrOScOpy
ABSTRACT Healing wounds and cancers present remarkable cellular molecular parallels, but the specific roles of healing phases are largely unknown. We developed a bioinformatics pipeline to identify genes pathways that define distinct across time course healing. Their comparison cancer transcriptomes revealed resolution-phase wound signature is associated with increased severity in skin enriches for extracellular matrix-related pathways. Comparisons early- late-phase fibroblasts vs...