One of the earliest neuropathological events in Alzheimer's disease is accumulation astrocytes at sites Aβ 1-42 depositions.Our results indicate that toxic peptide increases lipid peroxidation, apoptosis and cell death neurons but not primary culture.Aβ -induced deleterious neuronal effects are present when mixed cultured.Stimulation with increased p-65 decreased IκB resulting inflammatory process.In decreases protein expressions sirtuin 1 (SIRT-1) peroxisome proliferator-activated receptor...

Abstract Aim To evaluate the effects of photodynamic therapy ( PDT ) in nonsurgical treatment chronic periodontitis. Materials and methods A randomized, single‐blind, controlled, parallel‐group clinical trial was performed. Sixty patients were enrolled: 20 healthy controls 40 with The randomized for scaling root planing SRP or + . Periodontal (plaque index, probing depth, recession, attachment level, bleeding on gingival crevicular fluid volume, corresponding to 381 versus 428 critical...

Alzheimer´s disease (AD), a neurodegenerative illness involving synaptic dysfunction with extracellular accumulation of Aβ1-42 toxic peptide, glial activation, inflammatory response and oxidative stress, can lead to neuronal death. Endogenous cannabinoid system is implicated in physiological physiopathological events central nervous (CNS), changes this are related many human diseases, including AD. However, studies on the effects cannabinoids astrocytes functions scarce. In primary cultured...

The aim of the present study was to investigate in rat mesenteric artery rings whether low concentrations vasopressin could modify contractile responses noradrenaline and electrical stimulation perivascular nerves. Vasopressin (10 −10 –10 −7 M ) caused concentration‐dependent contractions (pD 2 =8.36±0.09). V 1 ‐receptor antagonist d(CH 5 Tyr(Me)AVP −9 −8 produced parallel rightward shifts control curve for vasopressin. Schild analysis yielded a pA value 9.83 with slope 1.10±0.14. (3×10 10...

Background and Purpose —Accumulation of endogenous guanidino-substituted analogues l -arginine in chronic renal failure might contribute to some the vascular neurological disorders this pathology. We tested hypothesis that human cerebral arteries, guanidino compounds may increase tone, through nitric oxide (NO) synthase inhibition, impair endothelium-dependent relaxation. Methods —Rings middle artery were obtained during autopsy 26 patients who had died 3 12 hours before. The rings suspended...

Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after presence toxic peptide Aβ1-42 in cultured astrocytes remains poorly resolved.Here we use low-doses aspirin (10 -7 M) primary culture or absence peptide.We noted an increase cell viability proliferation with without treated cells.In addition, a decrease apoptosis, determined by Caspase 3 activity expression Cyt c Smac/Diablo, were detected.Also, diminished necrosis process...

The amyloid precursor protein plus presenilin-1 (APP/PS1) mice are a frequently-used model for Alzheimer's disease studies (AD).However, the data relevant to which proteins involved in inflammatory mechanism not sufficiently well-studied using AD mouse model.Using behavioral studies, quantitative RT-PCR and Western-blot techniques, significant findings were determined by expression of inflammation comparing APP/PS1 Wild type mice.Increased GFAP could be associated with elevation number...

1 The effects of vasopressin and deamino-8-D-arginine (DDAVP, desmopressin) were studied in artery rings (0.8-1 mm external diameter) obtained from portions human omentum during the course abdominal operations (27 patients). 2 In arterial under resting tension, produced concentration-dependent, endothelium-independent contractions with an EC50 0.59 ± 0.12 nm. V1 antagonist d(CH2)5Tyr(Me)AVP (1 μ) mixed V1-V2 desGly-d(CH2)5D-Tyr(Et)ValAVP (0.01 μm) displaced control curve to right a parallel...

Endothelin constricted human isolated cerebral arteries in a concentration‐dependent manner. The maximal tension developed, as well EC 50 values were similar with and without endothelium. Removal of extracellular calcium or addition the antagonist nicardipine (10 −6 m ), attenuated but did not abolish responses to endothelin. These experiments show that endothelin‐induced contraction is linked presence intact endothelial cells. data also contractile effects endothelin cannot be explained...

Sugammadex, a γ-cyclodextrin that encapsulates selectively steroidal neuromuscular blocking agents, such as rocuronium or vecuronium, has changed the face of clinical pharmacology.Sugammadex allows rapid reversal muscle paralysis.Sugammadex appears to be safe and well tolerated.Its blood-brain barrier penetration is poor (< 3% in rats), thus no relevant central nervous toxicity expected.However blood brain permeability can altered under different conditions (i.e.neurodegenerative diseases,...

Ranolazine (Rn) is an antianginal agent used for the treatment of chronic angina pectoris when not adequately controlled by other drugs. Rn also acts in central nervous system and it has been proposed pain epileptic disorders. Under hypothesis that ranolazine could act as a neuroprotective drug, we studied its effects on astrocytes neurons primary culture. We incubated rat cultures 24 hours with (10−7, 10−6 10−5 M). Cell viability proliferation were measured using trypan blue exclusion...

Arginine vasopressin (AVP) not only acts directly on blood vessels through V1 receptor stimulation but also may modulate adrenergic-mediated responses in animal experiments vivo and vitro. The aim of the present study was to investigate whether AVP can contribute an abnormal adrenergic constrictor response human saphenous veins.Saphenous vein rings were obtained from 32 patients undergoing coronary artery bypass surgery. suspended organ bath chambers for isometric recording tension....

Vasopressin not only acts directly on blood vessels through V1-receptor stimulation but also may modulate adrenergic-mediated responses in animal experiments vitro and vivo. The aim of the present study was to investigate whether subpressor concentrations vasopressin could modify constrictor norepinephrine electrical perivascular nerves human mesenteric arteries. Human artery rings (3-3.5 mm long, 0.8-1.2 OD) were obtained from 38 patients undergoing abdominal operations. arterial suspended...

Aims.Bifidobacterium pseudocatenulatum CECT 7765 improves metabolic and immunological altered functions in high fat fed mice, however little is known about the effects of potential probiotics on vascular reactivity.The aim present study was to investigate a probiotic strain, Bifidobacterium 7765, response obese mice.Methods.Aorta samples were obtained from which divided into three groups: control group, receiving standard diet; an high-fat group diet daily dose B. by oral gavage.Aortic rings...

The relaxant effect of 4-hydroxynonenal (4-HNE), a lipid peroxidation product, on human cerebral arteries was studied. Addition 4-HNE to artery rings promoted no contraction, and after stimulation with prostaglandin F 2α (PFG ; 10 −7 -3 × −6 M), 100% relaxation obtained 3 −5 M 4-HNE. Inhibition nitric oxide formation N G -nitro-l-arginine methyl ester hydrochloride (l-NAME; (10 −4 as well synthesis indomethacin (3 partially prevented 4-HNE-induced relaxation, but each these substances...

In Alzheimer's disease (AD), an increase in inflammation is distinctive. Amyloid precursor protein plus presenilin-1 (APP/PS1 mice) a model for this illness. Chemokines secreted by central nervous system (CNS) cells could play multiple important roles AD. Data looking the chemokines involved inflammatory mechanisms are lacking. To understand changes that occur process AD, it necessary to improve strategies act on specific targets.Chemokines and their receptors phagocytosis, demyelination,...