A5026569990- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- Neuroscience and Neuropharmacology Research
- Immune Response and Inflammation
- Sirtuins and Resveratrol in Medicine
- Fatty Acid Research and Health
- Peroxisome Proliferator-Activated Receptors
- Estrogen and related hormone effects
- Cell Adhesion Molecules Research
- Prenatal Substance Exposure Effects
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Sports injuries and prevention
- Neuropeptides and Animal Physiology
- Neurogenesis and neuroplasticity mechanisms
- Genomics, phytochemicals, and oxidative stress
- Alcohol Consumption and Health Effects
- Cancer, Stress, Anesthesia, and Immune Response
- Proteoglycans and glycosaminoglycans research
- Redox biology and oxidative stress
- Amyotrophic Lateral Sclerosis Research
- Bioactive Compounds and Antitumor Agents
- Bioactive natural compounds
- Cardiac Ischemia and Reperfusion
Universitat de València
2015-2024
INCLIVA Health Research Institute
2018-2020
Hospital Clínico Universitario de Valencia
2017
Centro de Investigacion Principe Felipe
2005
Instituto Valenciano de Investigaciones Agrarias
2003
University of Sheffield
1999-2002
Royal Hallamshire Hospital
1999
Abstract: The effect of chronic and in vitro ethanol exposure on brain oxygen radical formation lipid peroxidation was analyzed. Ethanol induces a dose‐dependent increase homogenates. peroxidative effects alcohol seem to be related both cytochrome P450 the ethanol‐inducible form (CYP2E1), because preincubation with metyrapone (an inhibitor P450) or an antibody against CYP2E1 abolished ethanol‐increased peroxidation. Using dichlorofluorescein, we also demonstrated that significantly enhanced...
Inflammatory processes and cytokine expression have been implicated in the pathogenesis of several neurodegenerative disorders. Chronic ethanol intake induces brain damage, although mechanisms involved this effect are not well understood. We tested hypothesis that activation glial cells by would induce stimulation signaling pathways inflammatory mediators brain, cause neurotoxicity. used cerebral cortex from control chronic ethanol‐fed rats, which received ethanol‐liquid diet for 5 months...
Abstract Activated astroglial cells are implicated in neuropathogenesis of many infectious and inflammatory diseases the brain. A number mediators cytokines have been proposed to play a key role glial cell-related brain damage. Cytokine production seems be initiated by signaling through TLR4/type I IL-1R (IL-1RI) response their ligands, LPS IL-1β, playing vital roles innate host defense against infections, inflammation, injury, stress. We shown that stimulated ethanol, up-regulating...
Abstract: We demonstrate the presence of cytochrome P4502E1 (CYP2E1) in astrocytes primary culture, its induction by ethanol, and concomitant generation free radical species. Double immunofluorescence using anti‐CYP2E1 anti‐glial fibrillary acidic protein showed that CYP2E1 was distributed over cytoplasm processes, although labeling more pronounced nuclear membrane. Immunogold confirmed this pattern distribution. Addition 25 m M ethanol to astrocyte culture medium for 14 days resulted an...
Abstract In the present study we analyze events which occur during early stages of astrogliogenesis by examining pattern both GFAP and vimentin gene expression their corresponding immunoreactive proteins rat brain development. This was carried out “in vivo” (whole brain) vitro” (primary culture radial glia) using immunofluorescence, immunoblotting, Northern blot analysis. Our results demonstrate that although immunostaining appeared late in gestation at day 5 glia cultures, mRNA first...
One of the earliest neuropathological events in Alzheimer's disease is accumulation astrocytes at sites Aβ 1-42 depositions.Our results indicate that toxic peptide increases lipid peroxidation, apoptosis and cell death neurons but not primary culture.Aβ -induced deleterious neuronal effects are present when mixed cultured.Stimulation with increased p-65 decreased IκB resulting inflammatory process.In decreases protein expressions sirtuin 1 (SIRT-1) peroxisome proliferator-activated receptor...
Abstract: Exposure to ethanol during fetal development reduces the astroglial‐specific marker glial fibrillary acidic protein (GFAP) and its mRNA levels in brains of rats radial glia primary culture, affecting proliferation differentiation astrocytes. The objectives this study were evaluate possible effect on GFAP astrocytes investigate molecular mechanism(s) involved ethanol‐induced changes expression by analyzing transcription rate, stability, DNA methylation. We show here that prenatal...
The CNS is particularly susceptible to the effects of alcohol and toxicity. Astrocytes are immunoactive cells, activation these cells associated with several neurodegenerative disorders. By using cultured cortical astrocytes, we show that a short ethanol treatment (100 mM) able up-regulate both cyclooxygenase 2 (COX-2) inducible nitric oxide synthase (iNOS) expression, regulated via nuclear factor kappa B (NF-kappa B) as revealed by inhibition NF-kappa pyrrolidine dithiocarbamate (PDTC) or...
Summary Oestrogenic compounds have been postulated as neuroprotective agents. This prompted us to investigate their mechanism action in neurons primary culture. Cells were pretreated with physiological concentrations of 17‐β estradiol (0.2 n m ) or nutritionally relevant genistein (0.5 µ ), and 48 h later treated 5 amyloid beta (Aβ) for 24 h. We found that Aβ increased oxidative stress, measured peroxide levels oxidized glutathione/reduced glutathione ratio, which turn, caused...
Abstract Background Interleukin (IL)-6 (mainly of tumor origin) activates glutathione (GSH) release from hepatocytes and its interorgan transport to B16-F10 melanoma metastatic foci. We studied if this capacity overproduce IL-6 is regulated by cancer cell-independent mechanisms. Methods Murine cells were cultured, transfected with red fluorescent protein, injected i.v. into syngenic C57BL/6J mice generate lung liver metastases, isolated foci using high-performance cell sorting. Stress...
Abstract Aim To evaluate the effects of photodynamic therapy ( PDT ) in nonsurgical treatment chronic periodontitis. Materials and methods A randomized, single‐blind, controlled, parallel‐group clinical trial was performed. Sixty patients were enrolled: 20 healthy controls 40 with The randomized for scaling root planing SRP or + . Periodontal (plaque index, probing depth, recession, attachment level, bleeding on gingival crevicular fluid volume, corresponding to 381 versus 428 critical...
Alzheimer´s disease (AD), a neurodegenerative illness involving synaptic dysfunction with extracellular accumulation of Aβ1-42 toxic peptide, glial activation, inflammatory response and oxidative stress, can lead to neuronal death. Endogenous cannabinoid system is implicated in physiological physiopathological events central nervous (CNS), changes this are related many human diseases, including AD. However, studies on the effects cannabinoids astrocytes functions scarce. In primary cultured...
Polyphenolic phytochemicals have anticancer properties. However, in mechanistic studies, lack of correlation with the bioavailable concentrations is a critical issue. Some reports had suggested that these molecules downregulate stress response, which may affect growth and antioxidant protection malignant cells. Initially, we studied this potential underlying mechanism using different human melanomas (with genetic backgrounds correlating most melanomas), growing nude mice as xenografts,...
We showed previously that alcohol exposure during in vivo brain development induced astroglial damage and caused cell death. Because ceramide modulates a number of biochemical cellular responses to stress, including apoptosis, we now investigate whether ethanol-induced death astrocytes is mediated by signalling pathways triggering apoptosis. Here show both ethanol are able induce apoptotic cultured astrocytes, dose-dependent manner, C2-ceramide addition potentiates the effects ethanol. Cell...
Abstract: The alterations in astrocyte proliferation and differentiation induced by prenatal exposure to alcohol (PEA) suggest that ethanol affects the radial glial cells, main astrocytic precursors. We have investigated effects of on early stages astrogliogenesis analyzing developmental pattern vimentin fibrillary acidic protein (GFAP) immunoreactivity their mRNA levels during embryonic/fetal brain development glia primary culture. GFAP appeared late gestation at day 5 culture glial,...
Abstract Astroglial cells play an important role in maintaining neuronal function the adult and developing nervous system. Ethanol exposure induces profound alterations astrogliogenesis process, affecting cell functions, including intracellular protein trafficking. Because actin cytoskeleton plays a crucial transport, aim of present study was to analyze effects ethanol on organization involvement RhoA signaling pathway these effects. We show that lysophosphatidic acid (LPA), upstream...