One of the earliest neuropathological events in Alzheimer's disease is accumulation astrocytes at sites Aβ 1-42 depositions.Our results indicate that toxic peptide increases lipid peroxidation, apoptosis and cell death neurons but not primary culture.Aβ -induced deleterious neuronal effects are present when mixed cultured.Stimulation with increased p-65 decreased IκB resulting inflammatory process.In decreases protein expressions sirtuin 1 (SIRT-1) peroxisome proliferator-activated receptor...

Abstract Aim To evaluate the effects of photodynamic therapy ( PDT ) in nonsurgical treatment chronic periodontitis. Materials and methods A randomized, single‐blind, controlled, parallel‐group clinical trial was performed. Sixty patients were enrolled: 20 healthy controls 40 with The randomized for scaling root planing SRP or + . Periodontal (plaque index, probing depth, recession, attachment level, bleeding on gingival crevicular fluid volume, corresponding to 381 versus 428 critical...

Alzheimer´s disease (AD), a neurodegenerative illness involving synaptic dysfunction with extracellular accumulation of Aβ1-42 toxic peptide, glial activation, inflammatory response and oxidative stress, can lead to neuronal death. Endogenous cannabinoid system is implicated in physiological physiopathological events central nervous (CNS), changes this are related many human diseases, including AD. However, studies on the effects cannabinoids astrocytes functions scarce. In primary cultured...

Telemedicine and all types of monitoring systems have proven to be a useful low-cost tool with high level applicability in cardiology. The objective this work is present an IoT-based system for cardiovascular patients. sends the ECG signal Fog layer service by using LoRa communication protocol. Also, it includes AI algorithm based on deep learning detection Atrial Fibrillation other heart rhythms. automatic arrhythmias can complementary diagnosis made physician, achieving better clinical...

Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after presence toxic peptide Aβ1-42 in cultured astrocytes remains poorly resolved.Here we use low-doses aspirin (10 -7 M) primary culture or absence peptide.We noted an increase cell viability proliferation with without treated cells.In addition, a decrease apoptosis, determined by Caspase 3 activity expression Cyt c Smac/Diablo, were detected.Also, diminished necrosis process...

The amyloid precursor protein plus presenilin-1 (APP/PS1) mice are a frequently-used model for Alzheimer's disease studies (AD).However, the data relevant to which proteins involved in inflammatory mechanism not sufficiently well-studied using AD mouse model.Using behavioral studies, quantitative RT-PCR and Western-blot techniques, significant findings were determined by expression of inflammation comparing APP/PS1 Wild type mice.Increased GFAP could be associated with elevation number...

Ranolazine (Rn) is an antianginal agent used for the treatment of chronic angina pectoris when not adequately controlled by other drugs. Rn also acts in central nervous system and it has been proposed pain epileptic disorders. Under hypothesis that ranolazine could act as a neuroprotective drug, we studied its effects on astrocytes neurons primary culture. We incubated rat cultures 24 hours with (10−7, 10−6 10−5 M). Cell viability proliferation were measured using trypan blue exclusion...

Rocuronium (ROC) and Vecuronium (VEC) are the most currently used steroidal non-depolarizing neuromuscular blocking (MNB) agents. Sugammadex (SUG) rapidly reverses NMB agents after anaesthesia. The present study was conducted in order to evaluate neuronal effects of SUG alone combination with both ROC VEC. Using MTT, CASP-3 activity Western-blot we determined toxicity SUG, or VEC neurons primary culture. induces apoptosis/necrosis culture increases cytochrome C (CytC), apoptosis-inducing...

Nanoparticles have a promising future in biomedical applications and knowing whether they affect ex vivo vascular reactivity is necessary step before their use patients. In this study, we evaluated the effect of cerium dioxide nanoparticles (CeO2NPs) on human saphenous vein response to relaxing contractile agonists. addition, measured protein expression key enzymes related homeostasis oxidative stress. We found that CeO2NPs increased both SOD isoforms, consequent reduction superoxide anion...

Some of the beneficial effects exercise in preventing vascular related diseases are mediated by enhancement endothelial function where role nitric oxide (NO) is well documented, although relevance calcium activated potassium channels not fully understood. The impact oxidative stress induced training on remains to be clarified. By evaluating different vasodilator pathways two beds a rabbit model chronic exercise, we found decreased NO bioavailability and synthase expression both carotid...

Ranolazine improves vascular function in animal models. We evaluate the effects of ranolazine on and adrenergic response human saphenous vein.Rings from 53 patients undergoing coronary artery bypass grafting were mounted organ baths. Concentration-response curves to constructed rings precontracted with phenylephrine, endothelin-1, vasopressin, KCl thromboxane A2 analogue U-46619. In relaxation was tested absence presence endothelial factors inhibitors, K+ channel blockers verapamil. The...

Levamisole is an anthelmintic drug restricted to veterinary use but currently detected as the most widely used cocaine cutting agent in European countries. Levamisole-adulterated has been linked acute kidney injury, marked by a decrease glomerular filtration rate, which involves reduced renal blood flow, data on alteration of renovascular response produced levamisole are scarce. Renal arteries were isolated from healthy rabbits and for isometric tension recording organ baths protein...

Ante el alarmante aumento de la incidencia enfermedades mentales en población general, especialmente entre los jóvenes, propuesta "FarmaSalud Mental" busca sensibilizar a estudiantes del Grado Farmacia sobre importancia salud mental y empoderarlos como protagonistas activos búsqueda soluciones. Este proyecto se desarrolla seminarios asignatura Fisiopatología, tercer curso, concibe un espacio acción inmediata colaborativa. Los participantes abordan desafíos desde diversas perspectivas,...

El estudiantado del grado en Medicina tiene unos niveles de autoexigencia muy elevados. Sin embargo, su autonomía y autorregulación no están alineadas cuando acceden al grado, como se deduce diferentes estudios sobre estado ánimo sus tasas ansiedad. Diseñar experiencias que permitan mejorar aprendizajes basadas datos objetivos alienten trabajo es esencial. Presentamos materiales integración contenidos teórico-prácticos autoevaluación, ofrecen para el acceso libre por parte alumnado. En esta...

The increase of vascular arginase activity during aging causes endothelial dysfunction. This enzyme competes with the nitric oxide synthase (eNOS) for L-arginine substrate. Our hypothesis is that glucose 6-P dehydrogenase (G6PD) overexpression could improve function modulating pathway in aorta from mice. For this study, three groups male mice were used: young wild type (WT) (6-9 months), old WT (21-22 months) and G6PD-Tg Vascular reactivity results showed a reduced acetylcholine-dependent...

Underlying mechanisms associated with vascular dysfunction in metabolic syndrome (MetS) remain unclear and can even vary from one bed to another.In this study, MetS was induced by a high-fat, high-sucrose diet, after 28 weeks, aorta renal arteries were removed used for isometric recording of tension organ baths, protein expression Western blot, histological analysis assess the presence atherosclerosis.MetS mild hypertension, pre-diabetes, central obesity dyslipidaemia. Our results indicated...

Levamisole, a veterinary anthelmintic drug, is one of the most widely used and dangerous cocaine adulterants. Like cocaine, levamisole acutely blocks noradrenaline reuptake but with much less potency, although its vascular effects are not well known. In this study, we evaluated in rabbit aortic rings for isometric recording tension organ baths protein expression by western blot. Our results indicated that (10-5-10-3 M) induced concentration-dependent relaxation precontracted (10-7-3 × 10-7...

Ranolazine (RN) is a drug used in the treatment of chronic coronary ischemia. Different clinical trials have shown that RN behaves as an anti-diabetic by lowering blood glucose and glycosylated hemoglobin (HbA1c) levels. However, has not been to improve insulin (IN) sensitivity. Our study investigates possible facilitating effects on actions IN rabbit aorta. induced vasodilation abdominal aorta concentration-dependent manner, this dilatory effect was due phosphorylation endothelial nitric...