A5101854927- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Polyomavirus and related diseases
- Pancreatic function and diabetes
- Soft tissue tumor case studies
- Parvovirus B19 Infection Studies
- Autoimmune and Inflammatory Disorders Research
- Tumors and Oncological Cases
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Drug-Induced Hepatotoxicity and Protection
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Endoplasmic Reticulum Stress and Disease
- Rheumatoid Arthritis Research and Therapies
- Silymarin and Mushroom Poisoning
- Cancer Treatment and Pharmacology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Eosinophilic Disorders and Syndromes
- Metabolism, Diabetes, and Cancer
- CNS Lymphoma Diagnosis and Treatment
- Effects of Radiation Exposure
- T-cell and Retrovirus Studies
- Lung Cancer Treatments and Mutations
- Urologic and reproductive health conditions
- Cytomegalovirus and herpesvirus research
University of Helsinki
2024
Ain Shams University
2022
The Royal Free Hospital
2018-2019
University College London
2018-2019
Mansoura University
2011-2019
Imperial College Healthcare NHS Trust
2012-2016
Imperial College London
2010-2016
Hammersmith Hospital
2010-2016
Charing Cross Hospital
2013
Epstein-Barr virus (EBV) persistently infects more than 90% of the human population and is etiologically linked to several B cell malignancies, including Burkitt lymphoma (BL), Hodgkin (HL), diffuse large (DLBCL). Despite its growth transforming properties, most immune-competent individuals control EBV infection throughout their lives. encodes various oncogenes, 6 latency-associated EBV-encoded nuclear antigens, only EBNA3B completely dispensable for transformation in vitro. Here, we report...
Posttransplant lymphoproliferative disorders (PTLDs) are a group of diseases that range from benign polyclonal to malignant monoclonal lymphoid proliferations. They arise secondary treatment with immunosuppressive drugs given prevent transplant rejection. Three main pathologic subsets/stages evolution recognised: early, polymorphic, and monomorphic lesions. The pathogenesis PTLDs seems be multifactorial. Among possible infective aetiologies, the role EBV has been studied in depth, virus is...
Summary Distinguishing Burkitt lymphoma (BL) from B cell lymphoma, unclassifiable with features intermediate between diffuse large B‐cell (DLBCL) and BL (DLBCL/BL), DLBCL is challenging. We propose an immunohistochemistry fluorescent in situ hybridization (FISH) based scoring system that employed three phases – Phase 1 (morphology CD10 BCL2 immunostains), 2 (CD38, CD44 Ki‐67 immunostains) 3 (FISH on paraffin sections for MYC , BCL6 immunoglobulin family genes). The was evaluated 252...
Summary Both virus-associated haemophagocytic syndrome (HPS) and human immunodeficiency multi-centric Castleman's disease (HIV-MCD) induced by herpesvirus-8 (HHV-8) are extremely rare. We therefore wished to investigate their occurrence together, establish the degree of cytokine activation present. From a prospective cohort individuals with HIV-MCD, we investigated incidence outcomes HPS measured 15 inflammatory cytokines plasma HHV-8 viral loads before during follow-up. Of 44 patients...
Establish and validate optimal minimal immunohistochemistry panels for usage in a staged algorithmic manner precise diagnosis of B-cell lymphomas countries with limited resources. Suggest short immunostains to be used referring units that refer suspected specialist diagnostic centers resourceful countries.Significant proportion six has characteristic morphology requiring panel confirmatory immunostains. The rest would go through five different algorithms.812 cases which lymphoma or an...
Summary This study aimed to document the morphological and immunophenotypic features, describe diagnostic features of bone marrow ( BM ) involvement in human herpes virus 8 Multicentric Castleman disease HHV 8‐ MCD ). trephine biopsy BMTB specimens from 28 patients were revisited. Samples evaluated for expression CD 3, 20, 138, 68R, glycophorin C, 42b, 8‐latency‐associated nuclear antigen LANA 1), Epstein–Barr virus‐encoded small RNA light chains. Presence significant numbers 1 +...
Immediate early response 3 interacting-protein 1 (IER3IP1) is an endoplasmic reticulum (ER) resident protein, highly expressed in pancreatic cells and the developing brain cortex. Homozygous mutations IER3IP1 have been found individuals with microcephaly neonatal diabetes, yet underlying mechanism causing β-cell failure remains unclear. Here, we used differentiation of genome-edited stem into islet to elucidate molecular basis diabetes. Using clustered regularly interspaced short palindromic...
We investigated 26 B-cell post-transplant lymphoproliferative disorders (B-PTLD) and 15 human immunodeficiency virus-related aggressive lymphomas (HIV-BCL) from England that were associated with Epstein-Barr virus (EBV) for the polymorphic sequences of EBV-encoded nuclear antigen 3C (EBNA3C) gene to distinguish two different EBV strains. Type-A-EBV was identified in 92% B-PTLDS 53% HIV-BCL (P = 0.003). Among HIV-BCL, patients type-B-EBV had been HIV positive significantly longer when...
To characterize the microenvironment of classical Hodgkin lymphoma (cHL) in people living with human immunodeficiency virus (PLWH). The objective was to identify and then quantify immune cells present microenvironment.Ten samples cHL from PLWH were compared 10 general population using tissue microarray technology immunohistochemistry. Sections immunostained antibodies for CD30, CD3, CD4, CD8, CD20, CD68R, CD56, CD57, CD123, FoxP3 granzyme B. A statistically significant reduction CD4(+) T...
Purpose: The present study was undertaken to evaluate the protective and therapeutic effects of silymarin mesenchymal stem cells (MSCs) ameliorate damage caused by gamma radiation.Materials methods: MSCs were given intravenous injection male rats (1.4 × 107 cells), 1 day next radiation (4Gy). While, administered orally at a dose 70 mg/kg b. wt., 3 days before irradiation continued for 21 post irradiation.Results: After weeks post-irradiation, results revealed significant decline in red blood...
Merkel cell carcinoma (MCC) is a rare primary neuroendocrine of the skin, usually occurring at sun‐exposed sites in elderly people. Divergent differentiation MCC, although rare, has been reported previous case series. We describe two new cases MCC with divergent differentiation. Patient 1 was 96‐year‐old man scalp lesion; on biopsy, morphology and immunoprofile suggested squamous 2 an 87‐year‐old woman lesion her leg, originally as carcinoma, later showing extensive local recurrence. On...
Mammary myofibroblastoma (MFB) is a rare benign spindle neoplasm that affects both sexes with male predominance. It can exhibit wide range of histological patterns. We report case epithelioid/spindle MFB the female breast admixed, but distinct, foci cell lipoma. Whilst all cells within tumour expressed CD34, AR, ER, BCL2, and CD10, only those desmin lipomatous areas S100. This finding, to our knowledge, novel one has not been reported before.
Aim of the study is to investigate diffuse large B-cell lymphoma (DLBCL) for presence BCL3 gene rearrangement and protein expression correlate these with immunophenotypic subsets DLBCL. We aimed pathogenetic implication in DLBCL.Tissue microarray sections from 78 DLBCLs were evaluated using immunohistochemistry IGH Fluorescent situ hybridisation (FISH) split-apart probes. was positive 36/78 cases, which seen one case. Three additional cases showed evidence trisomy BCL3/chromosome 19, two...
Abstract Immunohistochemistry can sub‐classify diffuse large B‐cell lymphoma (DLBCL) into germinal centre like (GCB) and non‐GCB subtypes. The latter consists predominately of the activated subgroup in which nuclear factor kappa‐B activation is its characteristic. Expression cellular caspase 8 (FLICE)‐like inhibitory protein (cFLIP), a homologue, regulated by signalling, it main inhibitor Fas ligand apoptosis. To determine if cFLIP expression was confined to subtype, we studied 66 cases...
<p dir="ltr"><b>Abstract</b></p><p dir="ltr">Immediate early response 3 interacting-protein 1 (IER3IP1) is an endoplasmic reticulum resident protein, highly expressed in pancreatic cells and the developing brain cortex. Homozygous mutations <i>IER3IP1</i> have been found individuals with microcephaly neonatal diabetes, yet underlying mechanism causing beta cell failure remains unclear. Here, we utilized differentiation of genome edited-stem into...
A 59 year old man with a renal transplant and well controlled HIV infection presented an enlarging, firm, intermittently discharging crusted umbilical nodule (fig 1). He gave history of …
MANF is an endoplasmic reticulum resident protein that plays a crucial role in attenuating ER stress responses. Although indispensable for the survival and function of mouse beta cells, its precise human cell development unknown. Herein, we show lack humans results diabetes due to increased leading impaired function. We identified two patients from different families with childhood neurodevelopmental disorder associated homozygous loss-of-function mutations <i>MANF</i> gene. To...
Enthesitis is defined as inflammation of the tendon, ligament, or capsule insertion into bone. It considered an early and cardinal marker spondyloarthropathy (SpA) diagnosis. There a growing demand for more accurate methods to diagnose enthesitis possible efficient treatment delivery. Ultrasound (US) promising tool diagnosis enthesitis. Our study aimed at assessing correlation between US disease activity well evaluating ability Glasgow Scoring Index (GUESS) Sonographic Enthesitic (SEI)...
Peripheral neuropathy induced by chemotherapy is considered the most common neurological disorder associated with chemotherapy.Different sites are involved in mechanism of peripheral and multifactorial.68% cases develop CIPN during first month following start chemotherapy, 60% within 3 months after only 30% 6 months.CIPN caused chemotherapeutic agents which include taxanes, platinum analogs, vinca alkaloids.The clinical presentation includes multiple symptoms that may cause functioning...