A5010434139- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Machine Learning in Bioinformatics
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Cancer therapeutics and mechanisms
- Transgenic Plants and Applications
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Advanced NMR Techniques and Applications
- Escherichia coli research studies
- Microbial infections and disease research
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral gastroenteritis research and epidemiology
- Synthesis and Characterization of Heterocyclic Compounds
- Aquaculture disease management and microbiota
St. Jude Children's Research Hospital
2020-2024
Rutgers, The State University of New Jersey
2016-2023
Johnson University
2013-2022
Rutgers Sexual and Reproductive Health and Rights
2021
Hiroshima University
2008-2010
NMR studies can provide unique information about protein conformations in solution. In CASP14, three reference structures provided by solution methods were available (T1027, T1029, and T1055), as well a fourth data set of NMR-derived contacts for an integral membrane (T1088). For the targets with NMR-based structures, best prediction results ranged from very good (GDT_TS = 0.90, T1055) to poor 0.47, T1029). We explored basis these comparing all CASP14 models against experimental data. T1027,...
As little is known about antimicrobial resistance genes in fish farms, this study was conducted to monitor the incidence and prevalence of a wide range Gram-negative bacteria isolated from water samples taken farms northern part Egypt. Ninety-one out two hundred seventy-four (33.2%) non-repetitive isolates showed multidrug phenotypes harbored at least one gene. PCR DNA sequencing results that 72 (26.3%) contain tetracycline 19 (6.9%) were positive for class 1 integrons with 12 different gene...
Journal Article Molecular characterization of antimicrobial resistance in Salmonella isolated from animals Japan Get access A.M. Ahmed, Ahmed Laboratory Food Microbiology and Hygiene, Graduate School Biosphere Science, Hiroshima University, Higashi‐Hiroshima, JapanDepartment Bacteriology, Faculty Veterinary Medicine, Kafr El‐Sheikh El‐Sheikh, Egypt Search for other works by this author on: Oxford Academic Google Scholar Y. Ishida, Ishida T. Shimamoto Tadashi Shimamoto, Higashi‐Hiroshima...
Abstract CASP13 has investigated the impact of sparse NMR data on accuracy protein structure prediction. NOESY and 15 N‐ 1 H residual dipolar coupling data, typical that obtained for N, 13 C‐enriched, perdeuterated proteins up to about 40 kDa, were simulated 11 targets ranging in size from 80 326 residues. For several targets, two prediction groups generated models are more accurate than those produced using baseline methods. Real collected a de novo designed also provided predictors,...
Reminiscent of eukaryotic apoptotic programmed cell death, bacteria also contain a large number suicide genes, which are in general co-expressed with their cognate antitoxin genes. These systems called the toxin-antitoxin (TA) associated cellular dormancy, and play major roles biofilm formation persistent multidrug resistance many human pathogens. In recent years, study on TA system toxins has become hot topic due to health implications these by virtue role bacterial pathogenicity. Here we...
During monitoring of raw milk samples from healthy cows for the presence antibiotic resistant bacteria, one isolate Klebsiella pneumoniae strain HUF-100 was found to be oxyimino-cephalosporins and aztreonam. It carry a chromosomally-encoded extended-spectrum beta-lactamase that has not been described previously, namely SHV-60. Thus, it must expected this will spread further among food-producing animals thereby constitute reservoir resistance gene can transfer cause treatment problems humans....
Replacement of a specific amino acid residue in protein with nonnatural analogues is highly challenging because their cellular toxicity. We demonstrate for the first time replacement all arginine (Arg) residues canavanine (Can), toxic Arg analogue. All 5-base (UACAU) mRNA interferase from Bacillus subtilis (MazF-bs(arg)) were replaced Can by using single-protein production system Escherichia coli. The resulting MazF-bs(can) gained 6-base recognition sequence, UACAUA, RNA cleavage instead...
Significance There are many questions related to the origin of life and how we came rely on an almost universal system encode all as know it today. The genetic code is both robust redundant, yet also full interesting anomalies. Here explore one these anomalies, specifically existence two separate boxes/classes serine codons, AGU/C UCU/C/A/G. Unlike other synonymous codons encoding amino acid, for require a two-base substitution in order go from box remain serine. Deciphering this be will...
Toxin–antitoxin (TA) systems consist of a toxin inhibiting essential cellular functions (such as DNA, RNA and protein synthesis), its cognate antitoxin neutralizing the toxicity. Recently, we identified TA system termed TsbA/TsbT in Staphylococcus aureus genome. The induction tsbT gene Escherichia coli halted both DNA synthesis, reduced supercoiled plasmid resulted increasingly relaxed DNA. These results suggested that gyrase was target TsbT. In addition, TsbT inhibited E. S. activity...
Abstract Background Regional variation in Helicobacter pylori resistance patterns is a significant contributing factor for the ineffectiveness of traditional treatments. To improve treatment outcomes, we sought to create an individualized, susceptibility‐driven therapeutic approach among our patient population, which one poorest nation. It medically underserved, minority‐predominant and has high incidence H infection. Methods We compiled various factors involved antibiotic from literature....
Bacterial reverse transcriptase coding gene (RT) is essential for the production of a small satellite DNA-RNA complex called multicopy single-stranded DNA (msDNA). In this study, we found novel retron, retron-Vmi1 (Vm85) from Vibrio mimicus. The retron comprised msr-msd region, orf323, and ret gene, genetic organization similar to Salmonella's retron-Sen2 (St85). protein sequence RNA-directed polymerase (RT-Vmi1) highly homologous RTs metoecus, parahaemolyticus, vulnificus. Phylogenetic...
Bac7 (1–35) is an Arg- and Pro-rich peptide antibiotic, produced in bovine cells to protect them from microbial infection. It has been demonstrated inhibit the protein synthesis E. coli, leading cell death. Because of its toxicity, no cost effective methods have developed for production Escherichia coli potential clinical use. Here, we found a method suppress toxicity establish high expression system, which was fused C-terminal end S, major spore-coat Myxococcus xanthus, using linker...
NMR studies can provide unique information about protein conformations in solution. In CASP14, three reference structures provided by solution methods were available (T1027, T1029, and T1055), as well a fourth data set of NMR-derived contacts for integral membrane (T1088). For the targets with NMR-based structures, best prediction results ranged from very good (GDT_TS = 0.90, T1055) to poor 0.47, T1029). We explored basis these comparing all CASP14 models against experimental data. T1027,...