A5101693631- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Gut microbiota and health
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Nicotinic Acetylcholine Receptors Study
- Neuroendocrine regulation and behavior
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Cell Image Analysis Techniques
- Electrolyte and hormonal disorders
- Natural product bioactivities and synthesis
- Plant Disease Resistance and Genetics
- Sesquiterpenes and Asteraceae Studies
- Plant and Fungal Interactions Research
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Inflammatory Bowel Disease
- Protein Structure and Dynamics
- Animal Behavior and Welfare Studies
- Phytochemistry and Biological Activities
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Toxin Mechanisms and Immunotoxins
Zhengzhou University of Light Industry
2020-2025
Tianjin Normal University
2021-2024
Sutherland Hospital
2021-2024
UNSW Sydney
2021-2024
Ludong University
2024
Qinghai University for Nationalities
2022-2023
Beihua University
2023
Tokyo University of Agriculture and Technology
2012-2022
Tokyo University of Science
2022
Institute of Process Engineering
2019
Background and Aims The recruitment activation of inflammatory cells in the liver delineates transition from hepatic steatosis to steatohepatitis (SH). Approach Results We found that SH, γδT are recruited by C‐C chemokine receptor (CCR) 2, CCR5, nucleotide‐binding oligomerization domain‐containing protein 2 signaling skewed toward an interleukin (IL)‐17A + phenotype inducible costimulator (ICOS)/ICOS ligand–dependent manner. exhibit a distinct Vγ4 , PD1 Ly6C CD44 SH. Moreover, up‐regulate...
NMR studies can provide unique information about protein conformations in solution. In CASP14, three reference structures provided by solution methods were available (T1027, T1029, and T1055), as well a fourth data set of NMR-derived contacts for an integral membrane (T1088). For the targets with NMR-based structures, best prediction results ranged from very good (GDT_TS = 0.90, T1055) to poor 0.47, T1029). We explored basis these comparing all CASP14 models against experimental data. T1027,...
Abstract Gaussia luciferase (GLuc) is a small (18.2 kDa; 168 residues) and thus attracting much attention as reporter protein, but the lack of structural information hampering further application. Here, we report first solution structure fully active, recombinant GLuc determined by heteronuclear multidimensional NMR. We obtained natively folded bacterial expression efficient refolding using Solubility Enhancement Petide (SEP) tag. Almost perfect assignments GLuc’s 1 H, 13 C 15 N backbone...
Gaussia Luciferase (GLuc) is a renowned reporter protein that can catalyze the oxidation of coelenterazine (CTZ) and emit bright light signal. GLuc comprises two consecutive repeats form enzyme body central putative catalytic cavity. However, deleting C-terminal repeat only limited reduces activity (over 30% residual luminescence intensity detectable), despite being key part How does remaining (tGLuc) CTZ? To address this question, we built structural model tGLuc by removing from resolved...
Whereas arginine vasopressin binds to its receptor subtypes V<sub>1</sub>R and V<sub>2</sub>R with equal affinity of approximately 2 nM, nonpeptide antagonists interact differently subtypes. The antagonist binding site was mapped by site-directed mutagenesis at six selected amino acid positions, K100D, A110W, M120V, L175Y, R202S, F307I, predicted be involved in differences between V<sub>2</sub> R V<sub>1</sub>R. These mutations did not alter the for vasopressin. However, SR121463B...
An efficient enzyme immobilization strategy based on DNA directed hydrophilic polydopamine (PDA) modified magnetic nanoparticles was developed in this study.
Gaussia luciferase (GLuc 18.2kDa; 168 residues) is a marine copepod that emits bright blue light when oxidizing coelenterazine (CTZ). It helical protein where two homologous sequential repeats form anti-parallel bundles, each made of four helices. We previously identified hydrophobic cavity as prime candidate for the catalytic site, but GLuc's fast bioluminescence reaction hampered detailed analysis. Here, we used azacoelenterazine (Aza-CTZ), non-oxidizable (CTZ) analog, probe to investigate...
Inflammatory bowel disease (IBD) is a group of immune-mediated disorders characterised by chronic, relapsing-remitting inflammation predominantly affecting the gastrointestinal tract. IBD incurable, people in their most productive years. historically seen as Westernised nations although recent times other countries have an exponential rise cases. Although exact pathogenesis remains unclear, evidence suggests that microbiota changes play critical role pathogenesis. Over past two decades, has...
The gut microbiota of wild animals can regulate host physical health to adapt the environment. High-throughput sequencing from fecal samples was used analyze communities in common cranes (Grus grus) without harming them. Herein, we compared microbiome fifteen G. grus Tianjin Tuanbo Bird Natural Reserve (wild group) and six sampled Beijing Wildlife Park (semi-captive China, using 16S amplicon bioinformatic analysis. results showed that diversity composition varied different groups, suggesting...
Introduction Crohn’s disease and ulcerative colitis are common chronic idiopathic inflammatory bowel diseases (IBD), which cause considerable morbidity. Although the precise mechanisms of remain unclear, evidence implicates a strong multidirectional interplay between diet, environmental factors, genetic determinants/immune perturbations gut microbiota. IBD can be brought into remission using number medications, act by suppressing immune response. However, none available medications address...
ABSTRACT We report a novel bacterial screening protocol based on co‐expressing the target protein with VanX, an enzyme which mediates Escherichia coli 's autolysis and release of into culture medium, thereby facilitating activity measurement from crude medium. This as assessed 19 Gaussia luciferase (GLuc) expressing colonies, was able to detect bioluminescence wavelength shift small 1.5 nm. demonstrate performance versatility this by applying it semi‐rational search for GLuc variants...
Recent breakthroughs in the solution of X-ray structures for G protein-coupled receptors (GPCRs) with diffusible ligands have employed extensively mutated or recombined receptor fusion proteins heterologously expressed conventional vitro cell-based systems. While these advances now show that crystallization non-rhodopsin members this superfamily can be accomplished, use radically modified may limit relevance derived precision-guided drug design. To better enable study native GPCR structures,...
In the marine environment, phosphorus availability significantly affects lipid composition in many cosmopolitan heterotrophic bacteria, including members of SAR11 clade and Roseobacter clade. Under stress conditions, nonphosphorus sugar-containing glycoglycerolipids are substitutes for phospholipids these bacteria. Although play an important role as surrogates under phosphate deprivation, glycoglycerolipid synthases microbes poorly studied. present study, we biochemically characterized a...
The microbiome has been implicated in the development of metabolic conditions which occur at high rates people with schizophrenia and related psychoses. This exploratory proof-of-concept study aimed to: (i) characterize gut microbiota antipsychotic naïve or quasi-naïve first-episode psychosis, established receiving clozapine therapy; (ii) test for changes following a lifestyle intervention included diet exercise education physical activity. Participants were recruited from Eastern Suburbs...
The migratory bird’s gut microbiome composition and function change during the overwintering period, helping host to adapt different environments. Our study investigated of relict gulls ( Larus relictus ) in early late wintering stages from their grounds Tianjin, China. We collected 24 29 fecal samples at stages, respectively, analyzed using high-throughput sequencing technology find relationship between diet, living environment, birds. results showed that diversity abundance microbial...