A5100761784- Ferroptosis and cancer prognosis
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Click Chemistry and Applications
- Biochemical and Molecular Research
- Advanced MRI Techniques and Applications
- Cancer-related molecular mechanisms research
- Fluid Dynamics and Thin Films
- Ion Transport and Channel Regulation
- Intracranial Aneurysms: Treatment and Complications
- Acute Kidney Injury Research
- Nanoplatforms for cancer theranostics
- Schizophrenia research and treatment
- ATP Synthase and ATPases Research
- Immune cells in cancer
- CRISPR and Genetic Engineering
- Dialysis and Renal Disease Management
- Intracerebral and Subarachnoid Hemorrhage Research
- Fetal and Pediatric Neurological Disorders
- Biofuel production and bioconversion
- Cancer, Lipids, and Metabolism
- Circular RNAs in diseases
- Drug Transport and Resistance Mechanisms
- Mental Health and Psychiatry
- Chemical Synthesis and Analysis
Beijing National Laboratory for Molecular Sciences
2020-2024
Peking University
2020-2024
Harbin Medical University
2023-2024
First Affiliated Hospital of Harbin Medical University
2023-2024
Guangxi Medical University
2024
Northwestern Polytechnical University
2017
Xiangyang Central Hospital
2012
Itaconate is an anti-inflammatory metabolite involved in pathogen–macrophage interactions, but the mechanisms underlying its effect are not fully understood. Competitive cysteine profiling has been performed to interrogate itaconate's reactivity cell lysates, methods for analyzing targets of itaconation directly living macrophages still lacking. In this work, we developed a specific bioorthogonal probe, itaconate–alkyne (ITalk), quantitative and site-specific chemoproteomic inflammatory...
Itaconate is an important antimicrobial and immunoregulatory metabolite involved in host-pathogen interactions. A key mechanistic action of itaconate through the covalent modification cysteine residues via Michael addition, resulting "itaconation". However, it unclear whether has other regulatory mechanisms. In this work, we discovered a novel type post-translational by promiscuous antibody enrichment data analysis with open-search strategy further confirmed as lysine "itaconylation". We...
Sepsis associated Acute kidney injury (AKI) is a common clinical syndrome characterized by suddenly decreased in renal function and urinary volume. This study was designed to investigate the role of Aquaporin 1 (AQP1) P53 development sepsis-induced AKI their potential regulatory mechanisms. Firstly, transcriptome sequencing analysis mice showed AQP1 expression reduced elevated Cecal ligation puncture (CLP)-induced compared with controls. Bioinformatics confirmed that remarkably obviously...
Bioorthogonal probes have been developed to enable quantitative and site-specific profiling of itaconate modifications in <italic>Salmonella</italic>.
Diffusion Tensor Magnetic Resonance Imaging (DT-MRI, also known as DTI) measures the diffusion properties of water molecules in tissues and to date is one main techniques that can effectively study microstructures brain vivo. Presently, evaluation DTI registration still an initial stage development. In this paper, six well-known open source algorithms: Elastic, Rigid, Affine, DTI-TK, FSL SyN were applied on 11 subjects from open-access dataset, among which was randomly chosen template. Eight...
This study was designed to investigate the role of aquaporin 1 (AQP1) in ferroptosis, macrophage polarization, mitochondrial dysfunction, and impaired autophagy lipopolysaccharide (LPS)-stimulated RAW264.7 cells explored underlying mechanisms. Si-AQP1-mediated AQP1 silencing constructed. Si-P53-mediated P53 or pcDNA-P53 overexpression Assays ATP, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Mitochondrial membrane potential (JC-1) staining were performed evaluate...
Ferroptosis is an iron-related cell death caused by irregular lipid peroxidation that has been implicated with a variety of disease. Erastin canonical ferroptosis inducer known to function inhibiting system Xc– and cystine transport; however, the global interactome erastin in cells remains unexplored. In this work, we employed quantitative chemoproteomic approach profile direct interacting proteins living using multifunctional photo-cross-linking probe. A number novel erastin-interacting...
This paper proposes an adaptive numerical method for stochastic delay differential equations (SDDEs) with a non-global Lipschitz drift term and non-constant delay, building upon the work of Wei Fang others. The adapts step size based on growth term. Differing slightly from conventional Euler-Maruyama format, this addresses estimation by substituting it numerically obtained solution closest to left endpoint.This approach overcomes challenge nodes not falling within after subtracting delay....
Objective Investigate the value and feasibility of image registration with reverse rotation in lateral cerebral DSA.Methods ( 1 ) Experimental study:the target images were subtracted directly,and again after rotation.Software subtraction edited by author utilizing Visual Basic.The function automatic angle detection software evaluated to see whether it detected line.The DSA was evaluated.(2) Clinical retrospective untreated mask inages 15 patients motion along vertical axis during uploaded...
乳腺癌是造成女性死亡的主要原因之一，然而，其治疗及预后仍然面临重大挑战。胎盘特异性基因1(PLAC1)在胎盘以外的正常组织中基本不表达，但在多种肿瘤细胞中均有表达，在乳腺癌细胞中表达尤其丰富，可能与乳腺癌细胞的增殖、浸润及转移相关。PLAC1作为肿瘤特异性抗原，具有免疫原性，能诱导机体产生免疫应答，为乳腺癌的治疗提供了新的思路。针对PLAC1抗原表位的免疫治疗，可能具有重要的临床价值。笔者就PLAC1在乳腺癌中的研究现状进行介绍和分析。
目的本研究旨在从贵州木霉（Trichoderma guizhouense NJAU4742）菌株中克隆、表达几丁质酶基因chi8并深入研究其酶学特性，分析Chi8水解胶体几丁质的产物，为其在农业生产中的实际应用提供理论依据。 方法基于序列比对获取chi8与其他几丁质酶基因的同源关系，并设计引物扩增得到完整的chi8序列，利用pET-29a质粒和大肠杆菌BL21进行异源表达；经镍柱纯化后并研究Chi8酶学特性；利用MALDI-TOF/MS检测Chi8水解胶体几丁质的最终产物；基于同源建模法构建Chi8的三维模型并与几丁六糖进行对接获取其催化相关位点信息。 结果本试验克隆、表达得到的Chi8与哈茨木霉几丁质酶（ACM47359）的序列相似度为93.38%；Chi8在30~40℃酶活性较高且能保持较好的热稳定性，在pH6.0时酶活性为0.50...