In the fabrication of 3-D complex tissues for implantation, layer-by-layer (LBL) electrospun nanofibrous scaffolds have recently received intensive interest. However, poor cell adhesion and expansion between layers in an LBL stack remain important issues. this study, we report a mussel-inspired, biomimetic approach to functionalize surface PCL/gelatin membranes coated with poly (dopamine) (PDA). Our study demonstrates that PDA coating on nanofibers leads significant change their properties...

Abstract: In this study, we fabricated glial cell-line derived neurotrophic factor (GDNF)-loaded microspheres, then seeded the microspheres in gelatin-methacrylamide hydrogel, which was finally integrated with commercial bilayer collagen membrane (Bio-Gide ® ). The novel composite of nerve conduit employed to bridge a 10 mm long sciatic defect rat. GDNF-loaded gelatin had smooth surface an average diameter 3.9±1.8 µm. Scanning electron microscopy showed that were uniformly distributed both...

BackgroundDiffusion kurtosis imaging (DKI) is a technique based on the theory that diffusion of water follows non-Gaussian distribution; until now, few studies have explored usefulness DKI in differentiating parotid gland tumors. Material and MethodsDKI was performed 40 patients with tumors (33 benign, 7 malignant). parameters including apparent coefficient (Dapp) (Kapp) were obtained using DK model compared. Intraclass correlation (ICC) used to evaluate inter-observer agreement derived...

Background Preoperative accurate characterization of parotid gland tumors in different histologic types is crucial. T2 mapping has been proven to be useful for improving the accuracy tumor characterization. Purpose To evaluate ability imaging tumors. Material and Methods was scanned 74 patients (56 benign, 18 malignant) with pathologically confirmed Mean relaxation time calculated compared between benign malignant group, among tumors, Warthin’s pleomorphic adenomas. Independent-samples t...

To evaluate the feasibility of using imaging parameters (D, β and μ) obtained from fractional order calculus (FROC) diffusion model to differentiate salivary gland tumors.15 b-value (0-2000 s/mm2) diffusion-weighted (DWI) was scanned in 62 patients with tumors (47 benign 15 malignant). Diffusion coefficient D, parameter (which correlates tissue heterogeneity) a microstructural quantity μ solid portion within tumor were calculated, compared between malignant groups, or among pleomorphic...

ECC is a common clinical manifestation of the oral cavity in childhood and Iron deficiency-anemia (IDA) high-risk factor but extrinsic black stain on tooth surface protective for caries. There limited information about microecological change early children who suffer from with IDA (BS). This study enrolled 136 aged 3-6 years. Dental caries teeth BS were examined. Saliva was collected 16S rRNA gene fingertip blood Hemoglobin test. are 93 including 13 (IDA ECC) 80 without (NIDA 43 free (CF) 17...

Biomimetic surface modification of scaffolds with osteoinductive molecules is a promising strategy for improving the bioactivity scaffold and stimulating stem cell signals in bone tissue engineering. Zirconia (ZrO2) offers excellent mechanical properties biocompatibility, but its bio-inert nature hinders use regeneration applications. Recombinant human morphogenetic protein 7 (rhBMP-7) arginine-glycine-aspartate (RGD) peptide were investigated their potential as surface-modifying...

Abstract Background Esophageal caustic stricture is a stubborn disease and postoperative restenosis limits the clinical efficacy of endoscopic dilation. Autologous mucosal grafts have been successfully applied in treatment urethral prevention after extensive resection. We aimed to use autografting performed endoscopically treat refractory esophageal stricture. Methods Three patients with intractable corrosive were treated by combining dilation autologous transplantation. Results All...

Pyroptosis is widely involved in many diseases, including periodontitis. Nonetheless, the functions of pyroptosis-related genes (PRGs) periodontitis are still not fully elucidated. Therefore, we aimed to investigate role PRGs Three datasets (GSE10334, GSE16134, and GSE173078) from Gene Expression Omnibus (GEO) were selected analyze differences expression values between nonperiodontitis tissue samples using difference analysis. Following this, five hub (charged multivesicular body protein 2B,...

A new rapid chemometric method has been developed to identify the anterior and posterior roots of cauda equina nerves by near-infrared (NIR) diffuse reflectance spectroscopy. NIR spectra were measured using a Fourier transform spectrometer equipped with fiber-optic probe. The result revealed no observable difference in between root samples, but two could be identified cluster analysis based on differences their spectral features. overall accuracy model was 87.5%, for actual 95% 80%,...

To investigate the causality between periodontitis and non-alcoholic fatty liver disease (NAFLD) using a two-sample bidirectional Mendelian randomisation (MR) analysis.

Treating old mandibular fractures with malunion is challenging due to the need for osteotomy and interference between bone segments, complicating occlusal restoration. This technical note introduces a novel office-based digital workflow utilizing surgical guides. Preoperative computed tomography (CT) data were imported into MIMICS software virtual planning precisely realign fragments based on anatomical relationships. A pre-bent reconstruction plate was adapted 3-dimensional-printed mandible...

Existing studies predominantly focused on the relationship between periodontitis and type 2 diabetes mellitus (T2DM), with limited data association 1 (T1DM). This study aimed to examine impact of T1DM subgingival microbiome levels advanced glycation end-products (AGEs). Samples were collected from four groups: T1DM, (P), (DP), periodontally systemically healthy controls (Control). Subgingival composition AGE assessed using 16S rRNA gene sequencing enzyme-linked immunosorbent assay (ELISA),...

We examined the relationships between structural connectome and temporomandibular disorders (TMDs).

Treatment of a severely comminuted mandibular fracture is challenging. This technical note describes novel office-based workflow, combining virtual surgery planning with short-segment drilling guides. The authors reduced the fractures via planning. Then, reconstructed mandible model was printed using an in-house 3D printer. Next, reconstruction plate preformed according to shape surface, and position screw hole in surface determined. Finally, hand-made guides for transfer were fabricated...

A new prototype of hybrid silk fibroin and sodium alginate (SF-SA) based osteogenic hydrogel scaffold with a concentration 2.5% magnesium phosphate (MgP) gel was prepared the assistance an extrusion-based three-dimensional (3D) printing machine in this study. To determine optimum ratio MgP-based hydrogel, series physical biochemical experiments were performed to proper MgP two-dimensional films, as well cell compatibility these materials sequence. The SF-SA 2.5wt% (SF-SA/MgP) stood out then...

The aim of this study was to determine the feasibility using near infrared (NIR) spectroscopy identify motor and sensory fascicles. Using 12 adult Beagle dogs which were anesthetised generally sacrificed, canalis vertebralis opened both anterior posterior roots harvested from first lumbar vertebrae second sacral (L 1 –S 2 ). A total 126 collected. NIR diffuse reflectance spectra measured a Fourier transform (FT-NIR) spectrometer fitted with fibre-optic probe. average spectrum each sample...