Journal Article Chlorantraniliprole Resistance in the Diamondback Moth (Lepidoptera: Plutellidae) Get access Wei Gong, Gong 1Key Laboratory of Pesticide Toxicology and Application Technique, College Plant Protection, Shandong Agricultural University, Tai'an 271018, P. R. China. Search for other works by this author on: Oxford Academic PubMed Google Scholar Hui-Hui Yan, Yan Li Gao, Gao 2Medical Department Central Hospital, 271000, Yun-Yun Guo, Guo 3Agricultural Animal Bureau Pingliang, Gansu...

Plutella xylostella (L.) is a migratory species and an important insect pest of cruciferous crops worldwide, Chrysoperla sinica (Tjeder) predaceous agricultural forest pests in the field. Indoxacarb has two enantiomers: (+)-S-indoxacarb (-)-R-indoxacarb. This study was conducted to clarify selective toxicity sublethal effects both enantiomers on P. C. sinica. The isomer had greater acute sinica, while (-)-R-indoxacarb less low Lethal concentration 25 % (LC25) significant development,...

Diamondback moth, Plutella xylostella (L.; Lepidoptera: Plutellidae), is an important pest of crucifers worldwide. The extensive use diamide insecticides has led to P. resistance and this presents a serious threat vegetable production. We selected chlorantraniliprole (Rf) flubendiamide (Rh) strains with ratios 684.54-fold 677.25-fold, respectively. Rf Rh underwent 46 36 generations lab-selection for resistance, Low cross cyantraniliprole was found. Cross chlorfenapyr, tebufenozid, indoxacarb...

Using microtitration method, the relationship between Phenoloxidase activity and resistance of diamondback moth Plutella xylostella (Linnaeus) to novel insecticide butane-fipronil was determined in vitro. After selection tenth-generation by butane-fipronil, fourth instar larvae increased 83.80-fold as compared susceptible strain. resistant strain (PO(r)) 1.29-fold higher than one (PO(s)). However, Km optimum pH values were similar strains, which 1.11 mM 6.5, respectively. Both PO(r) PO(s)...

The lacewing, Chrysoperla sinica, is a predaceous insect that important in crop pest management. Chemical pesticides have adversely impacted species. Here we studied the effect of tolfenpyrad on C. sinica. acute toxicity to second-instar larvae was determined and indicated medium-risk insecticide. Sublethal concentrations (LC10, LC20, LC30) had effects development, reproduction, predatory ability When sinica were exposed sublethal tolfenpyrad, activities protective enzymes, such as...

Abstract The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. use chlorantraniliprole has been essential in the management DBM. However, many countries and areas, DBM become highly resistant to chlorantraniliprole. Three different strains, susceptible (S), chlorantraniliprole-selected (Rc), field-collected (Rb) strains/populations were studied for role phenoloxidase resistance development insecticide. By assaying activity (PO) three results showed...

Abstract Helicoverpa armigera , cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels targeted mutagenesis in bollworm. However, deletions be more desirable disrupt function...

The support vector machines (SVM), as a novel type of learning machine, were used to develop quantitative structure-mobility relationship (QSMR) model 58 aliphatic and aromatic carboxylic acids based on molecular descriptors calculated from the structure alone. Multiple linear regression (MLR) radial basis function neural networks (RBFNNs) also utilized construct nonlinear compare with results obtained by SVM. root-mean-square errors in absolute mobility predictions for whole data set given...

Abstract The kinetic parameters of partially purified phenoloxidase (PO, EC. 1.14.18.1) from the 5th instar larvae Pieris rapae (Lepidoptera) were determined, using L‐3, 4‐dihydroxyphenylalanine (L‐DOPA) as substrate. optimal pH and temperature enzyme for oxidation L‐DOPA determined to be at 7.0 42°C, respectively. was stable between 6.5 7.4 temperatures lower than 37°C. At 6.8 37°C, Michaelis constant ( K m ) maximal velocity V 0.80 μmol/L 1.84 μmol/ L/min, Tetra‐hexylresorcinol...

The diamondback moth (DBM), Plutella xylostella L., is an important pest of cruciferous vegetables, and population control mainly depends on chemical pesticides. Emamectin benzoate a highly effective insecticide used for controlling DBM. However, it unknown how the sublethal effects low concentration residues emamectin So development benzoate, at LC 5 , 10 20 with concentrations 0.014 mg/L, 0.024 mg/L 0.047 respectively, adult DBM their progeny were investigated in this study. pupal weight,...

Abstract BACKGROUND The complex preparation process and storage instability of nanoformulations hinders their development commercialization. In this study, nanocapsules loaded with abamectin were prepared by interfacial polymerization at room temperature ordinary pressure using the monomers epoxy resin (ER) diamine. potential mechanisms primary amine tertiary in influencing shell strength dynamic stability (Aba@ER) suspension system systematically researched. RESULTS catalyzed...

Abstract Insulin‐like peptides (ILPs) act as crucial reproductive neuropeptides in insects, regulating insect reproduction through the insulin signaling pathway (ISP). Our previous studies have found that sublethal concentrations (LC 1 and LC 10 ) of lambda‐cyhalothrin ( λ Cy) could induce severe toxicity lacewing, Chrysoperla sinica (Tjeder), but toxicological mechanism remains unclear. This study discovered Cy inhibit CsILP transcription, leading to a decrease content downregulation C....

Abstract Diamondback moth (DBM), Plutella xylostella , is an important pest of crucifers worldwide. The extensive use diamide insecticides has led to DBM resistance in the world, and this presents a serious threat vegetable production. In present study, transcriptomic proteomic analyses were combined investigate potential flubendiamide‐resistance mechanism DBM. lab‐selected (R h ) field‐collected b flubendiamide‐resistant lines P. with ratios 1889.92‐fold 1250.97‐fold, respectively, used, as...

The effects of luteolin on phenoloxidase (PO) from Spodoptera exigua larvae were investigated with the use a microtitration assay in present paper. results showed that could inhibit activity PO and concentration this inhibitor led to 50% (IC50) reduction was estimated be 0.47 mmol/l. inhibitory kinetics analyzed by Lineweaver–Burk plots compound found reversible competitive Ki 13.11 obviously toxic against (≤3rd instar) exigua. highest percentage older (≥3rd greater than 60% treatment....

Laccase (EC 1.10.3.2) is known to oxidize various aromatic and nonaromatic compounds via a radical-catalyzed reaction, which generally includes two types of laccase, Lac1 Lac2. oxidizes toxic in the diet, Lac2 play an important role melanizing insect exoskeleton. In this study, we cloned sequenced cDNA diamondback moth, Plutella xylostella (PxLac2), from third instar larvae using polymerase chain reaction (PCR) rapid amplification ends techniques. The results showed that full-length PxLac2...

Polyphenol oxidase (PPO) is the enzyme responsible for enzymatic browning during growth of insects. It also involved in defense reactions and related with immunities PPO, a metalloenzyme oxidase, catalyzes oxidation o-diphenol to o-quinone. The present paper describes effects benzaldehyde its p-substituted derivatives on activity PPO from fifth instar Pieris rapae L. was purified using ammonium sulfate fractionation chromatography Sephadex G-100. kinetics characterized...