A5024859643- Autophagy in Disease and Therapy
- Chronic Kidney Disease and Diabetes
- Organ Transplantation Techniques and Outcomes
- Renal and related cancers
- Dialysis and Renal Disease Management
- Renal Transplantation Outcomes and Treatments
- Pancreatic function and diabetes
- Parathyroid Disorders and Treatments
- Diet and metabolism studies
- Potassium and Related Disorders
- Liver Disease and Transplantation
- Electrolyte and hormonal disorders
- Advanced MRI Techniques and Applications
- Alcohol Consumption and Health Effects
- Diabetes Treatment and Management
- MRI in cancer diagnosis
- Cell Adhesion Molecules Research
- Heme Oxygenase-1 and Carbon Monoxide
- Pediatric Urology and Nephrology Studies
- Gout, Hyperuricemia, Uric Acid
- Immune Cell Function and Interaction
- Cannabis and Cannabinoid Research
- Ion Transport and Channel Regulation
- Hydrogen's biological and therapeutic effects
- Biomedical Research and Pathophysiology
Osaka University
2010-2025
Otemae University
2024-2025
Advanced Processing Technology (United States)
2014
Excessive fat intake contributes to the progression of metabolic diseases via cellular injury and inflammation, a process termed lipotoxicity. Here, we investigated role lysosomal dysfunction impaired autophagic flux in pathogenesis lipotoxicity kidney. In mice, high-fat diet (HFD) resulted an accumulation phospholipids enlarged lysosomes within kidney proximal tubular cells (PTCs). isolated PTCs treated with palmitic acid, degradation activity progressively stagnated association...
Autophagy plays an essential role in cellular homeostasis through the quality control of proteins and organelles. Although a time-dependent decline autophagic activity is believed to be involved aging process, issue remains controversial. We previously demonstrated that autophagy maintains proximal tubular cell protects against kidney injury. Here, we extend study examine how aging. Unexpectedly, basal was higher aged than young kidney; short-term cessation tamoxifen-inducible...
Advanced glycation end products (AGEs) are involved in the progression of diabetic nephropathy. AGEs filtered by glomeruli or delivered from circulation endocytosed and degraded lysosomes kidney proximal tubular epithelial cells (PTECs). Autophagy is a highly conserved degradation system that regulates intracellular homeostasis engulfing cytoplasmic components. We have recently demonstrated autophagic damaged indispensable for cellular some settings. In this study, we tested hypothesis...
Key Points Restarting renin-angiotensin system inhibitor after discontinuation was associated with a lower risk of kidney outcomes and mortality but not related to hyperkalemia. Our findings support proactive approach restarting among patients CKD. Background While inhibitors (RASi) have been the mainstream treatment for CKD, they are often discontinued because adverse effects such as hyperkalemia AKI. It is unknown whether RASi improves clinical outcomes. Methods Using Osaka Consortium...
Chronic metabolic stress is related to diseases, whereas autophagy supplies nutrients by recycling the degradative products. Cyclosporin A (CsA), a frequently used immunosuppressant, induces via effects on mitochondrial respiration, and thereby, its chronic usage often limited. Here we show that plays protective role against CsA-induced in kidney proximal tubule epithelial cells. Autophagy deficiency leads decreased membrane potential, which coincides with abnormalities as characterized...
Metabolic acidosis, a common complication of CKD, causes mitochondrial stress by undefined mechanisms. Selective autophagy impaired mitochondria, called mitophagy, contributes toward maintaining cellular homeostasis in various settings. We hypothesized that mitophagy is involved proximal tubular cell adaptations to chronic metabolic acidosis. In transgenic mice expressing green fluorescent protein–tagged microtubule-associated protein 1 light chain 3 (GFP-LC3), NH4Cl loading increased the...
We encountered a family with hereditary renal failure, medullary cysts, pancreatic hypoplasia, hypomagnesemia, liver enzyme abnormalities, and diabetes mellitus (DM). identified novel heterozygous variant of HNF1B (NM_000458.4:c.791dup, p.L264Ffs*30) using whole-exome sequencing genomic DNA samples from this family. This is located in the DNA-binding domain protein produces truncated de novo sequence, suggesting that changes binding to or causes nonsense-mediated mRNA decay. Based on...
Renal ischemia-reperfusion (I/R) injury is unavoidable in kidney transplantation and frequently influences both short- long-term allograft survival rates. One of the major events I/R generation cytotoxic oxygen radicals. Recently, hydrogen gas has been reported to display antioxidant properties protective effects against organ dysfunction induced by various injuries. We investigated whether hydrogen-rich University Wisconsin (HRUW) solution attenuates renal cold injury.We prepared HRUW a...
Mesenchymal stem cells (MSCs) are an attractive therapeutic cell source for treating renal diseases. MSC administration has been shown to improve function, although the underlying mechanisms not completely understood. We recently showed that allogenic fetal membrane-derived MSCs (FM-MSCs), which available noninvasively in large amounts, had a renoprotective effect experimental glomerulonephritis model. Here we investigated whether FM-MSC could protect kidneys from ischemia/reperfusion (I/R)...
The serum glycoprotein fetuin-A is an important inhibitor of extraosseous calcification. importance has been confirmed in null mice, which develop widespread calcification including the kidney. However, mechanism how protects kidneys from nephrocalcinosis remains uncertain. Here, we demonstrate that intratubular plays a role prevention proximal tubules. Although normal rat kidney did not express mRNA for fetuin-A, found punctate immunohistochemical staining mainly S1 segment pattern...
Inhibiting tubular urate reabsorption may protect the kidney from urate-induced injury. However, this approach promote intratubular uric acid crystallization, especially in acidified urine, which could be toxic to kidney. To assess how handling affects outcomes, we conducted a retrospective cohort study including 1042 patients with estimated glomerular filtration rates (eGFR) of 15-60 mL/min/1.73 m
Medullary cystic kidney disease Type 1 is an autosomal dominant tubulointerstitial (ADTKD). Recently, mucin (MUC1) was identified as a causal gene of medullary (ADTKD-MUC1). However, the MUC1 mutation found to be single cytosine insertion in copy GC-rich variable number tandem repeats (VNTRs), which are very difficult analyze by next-generation sequencing. To date, other mutations have not been detected ADTKD-MUC1, and mutant protein has analyzed because difficulty genetically modifying VNTR...
To identify the mediators between canagliflozin and renoprotection in patients with type 2 diabetes at a high risk of end-stage kidney disease (ESKD).In this post hoc analysis CREDENCE trial, effect on potential (42 biomarkers) 52 weeks association changes renal outcomes were evaluated using mixed-effects Cox models, respectively. The outcome was composite ESKD, serum creatinine doubling or death. percentage mediating each significant mediator calculated based hazard ratios after additional...
Background Interstitial eosinophilic aggregates are observed in various kidney diseases, but their clinical implications remain unknown. We assessed the association between interstitial and outcomes further analyzed blood eosinophil count, as a surrogate for aggregates, risk of failure patients with advanced CKD. Methods datasets from two retrospective cohort studies: ( 1 ) biopsy including 563 who underwent native at Osaka University Hospital 2009 2021 2 CKD 2877 an eGFR 10–60 ml/min per...
Recent studies have identified T cells and natural killer (NKT) as important mediators in renal ischemia–reperfusion (I/R) injury. The recruitment of these is induced by chemotaxis factors. We investigated the effects blocking CXCR3 CCR5 an antagonist (TAK) using a rat I/R injury model. Sprague–Dawley rats were either subjected to sham operation or left occlusion for 45 min followed reperfusion contralateral nephrectomy. control TAK groups were, respectively, injected phosphate-buffered...
Abstract Background and Aims Patients with Sjögren's syndrome (SS) may occasionally present monoclonal gammopathy of undetermined significance (MGUS), the rare IgA type. Additionally, they tubulointerstitial nephritis (TIN) infiltrated by polyclonal plasmacytes lymphocytes. Previous reports indicate development IgA-type MGUS TIN plasma cells in some SS patients, which are considered distinct from renal (MGRS) because there is no evidence that affects nephropathy. Although treatment...
Metabolic acidosis is one of the most common complications chronic kidney disease (CKD). It associated with progression CKD, and many other functional impairments. Until recently, only serum bicarbonate levels have been used to evaluate acid-base changes in patients reduced function. However, recent emerging evidence suggests that nephrologists should reevaluate clinical approach for diagnosing metabolic CKD based on two perspectives; pH anion gap. Biochemistry physiology textbooks clearly...