Abstract Surface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In past two decades, SPR technology has been successfully commercialized its performance continuously improved lots engineering efforts. this review, we describe recent advances in technologies. The developments technologies focusing on speed, sensitivity,...

Face detection and alignment in unconstrained environment is always deployed on edge devices which have limited memory storage low computing power. This paper proposes a one-stage method named CenterFace to simultaneously predict facial box landmark location with real-time speed high accuracy. The proposed also belongs the anchor-free category. achieved by (a) learning face existing possibility semantic maps, (b) bounding box, offsets, five landmarks for each position that potentially...

Abstract Circular RNAs (circRNAs), often dysregulated in a variety of human diseases, participate the initiation and development cancers. Recently, circMTO1 (a circRNA derived from MTO1 gene), identified as tumor suppressor, has been shown to contribute suppression hepatocellular carcinoma. The present study aimed explore clinical significance roles liver fibrosis. Here, we found that serum was significantly down‐regulated chronic hepatitis B (CHB) patients. Interestingly, negatively...

A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza and B, H1N1, syncytial virus (RSV), parainfluenza 1-3 (PIV1, 2, 3), adenovirus,

The coronavirus disease 2019 (COVID-19) pandemic, caused by the new severe acute respiratory syndrome 2 (SARS-CoV-2) virus, has posed a threat to public health worldwide. Also, influenza virus large number of deaths annually. Since co-infection SARS-CoV-2 and which share similar symptoms, hampers current treatment efficiency, multiple simultaneous detection these viruses is needed provide right for patients. We developed microfluidic disc-direct RT-qPCR (dirRT-qPCR) assay rapid multiplex...

Surface initiated polymerization (SIP) has become an attractive method for tailoring physical and chemical properties of surfaces a broad range applications. Most those applications relied on the merit high density coating. In this study, we explored long overlooked field SIP: SIP from substrates low initiator density. We combined ellipsometry with AFM to investigate effect time morphology polymer coatings. addition, carefully adjusted nanoscale separation chains achieve balance between...

More than a decade after the outbreak of human coronaviruses (HCoVs) SARS in Guangdong province and Hong Kong SAR China 2002, there is still no reoccurrence, but evolution recombination this region are unknown. Therefore, surveillance on prevalence virus variation HCoVs circulation conducted. A total 3298 nasopharyngeal swabs samples were collected from cross-border children (<6 years, crossing border between Southern SAR) showing symptoms respiratory tract infection, such as fever (body...

This study tested the efficacy of xenomonitoring using contaminated flies and cockroaches at ports in Shenzhen by analysing sample data from imported October 2023 to April 2024 identify pathogens they carried. Among all samples collected, Musca domestica vicina Blattella germanica accounted for highest proportion, 27.59% 66.47%, respectively. Their positive rates carrying Staphylococcus aureus were also most significant, reaching 4.35% 6.47%, The mainly came Asia, with proportion coming Hong...

Influenza is a serious worldwide disease that captures global attention in the past few years after outbreaks. The recent discoveries of microRNA (miRNA) and its unique expression profile influenza patients have offered new method for early diagnosis. aim this study was to examine utility miRNAs diagnosis influenza.Thirteen selected were investigated with hosts' throat swabs (25 H1N1, 20 H3N2, B 21 healthy controls) by real-time quantitative polymerase chain reaction (RT-qPCR) using U6 snRNA...

ObjectiveThe nucleic acid-based polymerase chain reaction (PCR) assay is commonly applied to detect infection with Zika virus (ZIKV). However, the time- and labor-intensive sample pretreatment required remove inhibitors that cause false-negative results in clinical samples impractical for use resource-limited areas. The aim was develop a direct reverse-transcription quantitative PCR (dirRT-qPCR) ZIKV diagnosis directly from samples.MethodsThe combination of inhibitor-tolerant polymerases,...

Electronic component recognition plays an important role in industrial production, electronic manufacturing, and testing. In order to address the problem of low recall accuracy traditional image technologies (such as principal analysis (PCA) support vector machine (SVM)), this paper selects multiple deep learning networks for testing optimizes SqueezeNet network. The then presents algorithm based on Faster This structure can reduce size network parameters computational complexity without...

We have developed a pulsed xenon ultraviolet light-based real-time air disinfection system with rapid and effective by using high-intensity pulse germicidal UV. Disinfection of the ambulance's environment is critical in prevention infectious cross contamination. In this study, was established for ambulances. system, (PX-UV) used to generate broad-spectrum (200-320 nm), light deactivate kill bacteria viruses. The results showed that use PX-UV could be reducing E. coli, Staphylococcus albus,...

Ultrathin polymer brushes play important roles in natural and artificial systems. To better understand utilize their unique behaviors, characterization is a fundamental, but not trivial, task. In this paper, we demonstrated that the quartz crystal microbalance with dissipation (QCM-D) could be applied to study ultrathin poly(oligo(ethylene glycol) methacrylate) brushes. First, identified four linear relations between dissipation/frequency changes thickness changes, which were measured by...

Abstract Linear B-cell epitopes are ideal biomarkers for the serodiagnosis of infectious diseases. However, long-predicted diagnostic value has not been realized. Here, we demonstrated a method, in four steps (DEIFS), that delivers combination diseases with high success rate. Using DEIFS malaria, identified 6 from 8 peptides and combined them into 3 chimeric peptide constructs. Along 4 other peptides, developed rapid test (RDT), which is able to differentiate Plasmodium falciparum ( P. )...

A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using an acousto-optic tunable filter (AOTF) and a white light laser is presented. The combines the merits of wide-dynamic detection range high sensitivity offered by spectral approach with multiplexed high-throughput data collection two-dimensional (2D) array. key feature use AOTF to realize scan from source thus achieve tracking SPR dip movement caused target molecules binding sensor surface....

A weak polyelectrolyte coating, carboxylated poly(oligo(ethylene glycol)methacrylate-co-2-hydroxyethylmethacrylate), was prepared viasurface initiated polymerization (SIP) from initiators immobilized to gold surface through the Au–S bonds. When dry thickness increased up 75 nm, this coating pulled off Au substrate by simply exposing phosphate buffer saline (PBS, pH = 7.4, [Na+] 150 mM). This covalent bond breaking (CBB) behavior monitored in situ using a quartz crystal microbalance (QCM) and...

A wavelength-multiplexing phase-sensitive surface plasmon resonance (SPR) imaging sensor offering wide dynamic detection range and microarray capability is reported. Phase accomplished by performing self-interference between the s- p- polarizations within signal beam. liquid crystal tunable filter used to sequentially select SPR excitation wavelength from a white light source. This approach enables fast of sensor's phase response over wavelengths, thereby covering literally any regions...