A5057930854- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
Beth Israel Deaconess Medical Center
2024
Harvard University
2022-2024
University of Cambridge
2022-2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of current disease 2019 (COVID-19) pandemic, induces an unbalanced immune response in host. For instance, production type I interferon (IFN) and to it, which act as a front-line defense against virus invasion, are inhibited during SARS-CoV-2 infection. In addition, tumor necrosis factor alpha (TNF-α), proinflammatory cytokine, is upregulated COVID-19 patients with severe symptoms. Studies on closely related...
Classic Hodgkin Lymphoma (cHL) is a tumor composed of rare malignant and Reed-Sternberg (HRS) cells nested within T-cell rich inflammatory immune infiltrate. cHL associated with Epstein-Barr Virus (EBV) in 25% cases. The specific contributions EBV to the pathogenesis remain largely unknown, part due technical barriers dissecting microenvironment (TME) high detail. Herein, we applied multiplexed ion beam imaging (MIBI) spatial pro-teomics on 6 EBV-positive 14 EBV-negative samples. We identify...
The advent of spatial transcriptomics and proteomics have enabled profound insights into tissue organization to provide systems-level understanding diseases. Both technologies currently remain largely independent, emerging same slide multi-omics approaches are generally limited in plex, resolution, analytical approaches. We introduce IN-situ DEtailed Phenotyping To High-resolution (IN-DEPTH), a streamlined resource-effective approach compatible with various platforms. This iterative first...
SUMMARY The SARS-CoV-2 genome encodes a multitude of accessory proteins. Using comparative genomic approaches, an additional protein, ORF3c, has been predicted to be encoded within the ORF3a sgmRNA. Expression ORF3c during infection confirmed independently by ribosome profiling. Despite also being present in 2002-2003 SARS-CoV, its function remained unexplored. Here we show that localises mitochondria infection, where it inhibits innate immunity restricting IFN-β production, but not NF-κB...