A5100624608- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Advanced Breast Cancer Therapies
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- Cancer, Stress, Anesthesia, and Immune Response
- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- 3D Printing in Biomedical Research
- Telomeres, Telomerase, and Senescence
- Ovarian cancer diagnosis and treatment
- Connective tissue disorders research
- Connective Tissue Growth Factor Research
- Angiogenesis and VEGF in Cancer
- PI3K/AKT/mTOR signaling in cancer
- vaccines and immunoinformatics approaches
- Melanoma and MAPK Pathways
- RNA Interference and Gene Delivery
- Advancements in Transdermal Drug Delivery
National Taiwan Ocean University
2022-2024
Washington State University Spokane
2024
California Pacific Medical Center
2019-2023
Massachusetts Institute of Technology
2023
Scripps Research Institute
2023
Seattle University
2022
University of Washington
2022
Merck & Co., Inc., Rahway, NJ, USA (United States)
2021-2022
The University of Texas MD Anderson Cancer Center
2019-2021
Centre for Genomic Regulation
2021
Fibroblast growth factor 21 (FGF21) has emerged as an important metabolic regulator of glucose and lipid metabolism. The aims the current study are to evaluate role FGF21 in energy metabolism provide mechanistic insights into its lipid-lowering effects a high-fat diet-induced obesity (DIO) model.DIO or normal lean mice were treated with vehicle recombinant murine FGF21. Metabolic parameters including body weight, glucose, levels monitored, hepatic gene expression was analyzed. Energy insulin...
Circulating levels of fibroblast growth factor 21 (FGF21), a metabolic regulator glucose, lipid, and energy homeostasis, are elevated in obese diabetic subjects, raising questions about potential FGF21 resistance. Here we report tissue expression changes its receptor components, describe the target-organ whole-body responses to ob/ob diet-induced (DIO) mice. Plasma concentrations were 8- 16-fold DIO mice, respectively, paralleling dramatic increase hepatic mRNA expression. Concurrently,...
Early steps for cardiac specification are problematic the study of mammalian embryos, which has favored using pluripotent cells that recapitulate myogenesis. Furthermore, circuits governing have relevance to application ES and other heart repair. In mouse teratocarcinoma cells, canonical Wnts inhibit formation in avian or amphibian embryos explants activate cardiogenesis, paradoxically. Here, we show Wnt/β-catenin pathway also is essential myogenesis occur acting at a gastrulation-like...
Fibroblast growth factor 21 (FGF21) is a potent metabolic regulator, and pharmacological administration elicits glucose lipid lowering responses in mammals. To delineate if adipose tissue the predominant organ responsible for anti-diabetic effects of FGF21, we treated mice with reduced body fat (lipodystrophy specific expression active sterol regulatory element binding protein 1c; Tg) recombinant murine FGF21 (rmuFGF21). Unlike wildtype (WT) mice, Tg were refractory to beneficial rmuFGF21 on...
Clinical resistance to chemotherapy is a frequent event in cancer treatment and closely linked poor outcome. High-grade serous (HGS) ovarian characterized by p53 mutation high levels of genomic instability. Treatment includes platinum-based initial response rates are high; however, frequently acquired, at which point options largely palliative. Recent data indicate that platinumresistant clones exist within the sensitive primary tumor presentation, implying resistant cell selection after...
Expansion of extracellular matrix with fibrosis occurs in many tissues as part the end-organ complications diabetes, and advanced glycosylation end products (AGE) are implicated one causative factor diabetic tissue fibrosis. Connective growth (CTGF), also known insulin-like factor-binding protein-related protein-2 (IGFBP-rP2), is a potent inducer synthesis angiogenesis increased from rodent models diabetes. The aim this study was to determine whether CTGF up-regulated by AGE vitro explore...
The endocrine fibroblast growth factor 21 (FGF21) requires both receptor (FGFR) and β-Klotho for signaling. In this study, we sought to understand the inter-molecular physical interactions in FGF21/FGFR/β-Klotho complex by deleting key regions FGFR1c or FGF21. Deletion of D1 D1-D2 linker (the D1/linker region) from led β-Klotho-independent activation FGF21, suggesting that there may be a direct interaction between FGF21 region-deficient FGFR1c. Consistent with this, extracellular portion...
Abstract Cancer cell extravasation, a key step in the metastatic cascade, involves cancer arrest on endothelium, transendothelial migration (TEM), followed by invasion into subendothelial extracellular matrix (ECM) of distant tissues. While research has mostly focused biomechanical interactions between tumor cells (TCs) and ECM, particularly at primary site, very little is known about mechanical properties endothelial ECM how they contribute to extravasation process. Here, an integrated...
Summary Improvements in next‐generation sequencing technologies have resulted dramatically reduced costs. This has led to an explosion of ‘‐seq’‐based methods, which RNA ( ‐seq) for generating transcriptomic data is the most popular. By analysing global patterns gene expression organs/tissues/cells interest or response chemical environmental perturbations, researchers can better understand organism's biology. Tools designed work with large ‐seq sets enable analyses and visualizations help...
ABSTRACT Results of prior studies suggest that fibroblast growth factor 21 (FGF21) may be involved in bone turnover and the actions peroxisome proliferator-activated receptor (PPAR) α γ mice. We have conducted independent to examine effects FGF21 on homeostasis role PPARα actions. High-fat-diet-induced obesity (DIO) mice were administered vehicle or recombinant human (rhFGF21) intraperitoneally at 0 (vehicle), 0.1, 1, 3 mg/kg daily for 2 weeks. Additional groups DIO received water 10...
Expansion of extracellular matrix with fibrosis occurs in many tissues, including skin, as part the end-organ complications diabetes. Advanced glycosylation end-products (AGEs) have been implicated a pathogenic factor diabetic tissue fibrosis. Connective growth (CTGF), also known IGF-binding protein-related protein-2, induces matrix. We recently shown that CTGF mRNA and protein are up-regulated by AGE treatment cultured human dermal fibroblasts. The aim this study was to determine whether is...
Fibroblast growth factor 21 (FGF21) is a potent regulator of glucose and lipid homeostasis in vivo; its most closely related subfamily member, FGF19, known to be critical negative bile acid synthesis. To delineate whether FGF21 also plays functional role metabolism, we evaluated the effects short- long-term exposure native long-acting analogs on hepatic signal transduction, gene expression enterohepatic levels primary hepatocytes rodent monkey models. acutely induced ERK phosphorylation...
Abstract Infrared thermographs (IRTs) have been used for fever screening during infectious disease epidemics, including SARS, EVD and COVID-19. Although IRTs significant potential human body temperature measurement, the literature indicates inconsistent diagnostic performance, possibly due to wide variations in implemented methodology. A standardized method IRT was recently published, but there is a lack of clinical data demonstrating its impact on performance. We performed study 596...
Significance Durable protection against COVID-19 infection may be achieved by generating robust T cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and emerging SARS-CoV-2 variants; for those infected, effective treatments are urgently needed. For these strategies successful, accurate identification of epitopes is critical. In this study, we used major histocompatibility complex immune precipitation, acid elution, tandem mass spectrometry define the...
Homologous recombination DNA damage repair (HR-DDR) deficient patients with various solid tumors have been treated PARP inhibitors. However, the clinical characteristics of melanoma who HR-DDR gene mutations and consequences inhibition are poorly understood. We compared commercially available next-generation sequencing data from 84 melanomas our institution a dataset 1,986 as well 1,088 profiled in cBioportal. In total, 21.4% had ≥1 functional mutation, most commonly involving BRCA1, ARID1A,...
Glioblastoma’s (GBM) aggressive growth is driven by redundant activation of a myriad signaling pathways and genomic alterations in tyrosine kinase receptors, such as epidermal factor receptor (EGFR), which altered over 50% cases. Single agents targeting EGFR have not proven effective against GBM. In this study, we aimed to identify an anti-tumor regimen using pharmacogenomic testing patient-derived GBM samples, culture vivo. High-throughput pharmacological screens ten EGFR-driven samples...
The potential of lipid nanoparticles (LNPs) as nucleic acid delivery vehicles has been demonstrated in recent years, culminating the emergency use approval LNP-based mRNA SARS-CoV-2 vaccines late 2020. determination RNA content relative to LNP size can be important understanding efficacy and adverse effects. This work presents first description a facile rapid analytical method for online, size-dependent payload distribution measurement using data from multi-angle light scattering,...
Cten is a focal adhesion molecule that expressed at very low levels in most normal tissues. Nonetheless, its expression has been found to increase dramatically many types of cancer including colorectal, breast, gastric, and pancreatic cancer, suggesting cten may play critical role during tumorigenesis. To study the mechanisms induce function up-regulated cten, we examined effects several cancer-associated growth factors cytokines on expression. We EGF, FGF2, NGF, PDGF, TGF-β, IGF-1, IL-6,...
Abstract Inhalation of ricin toxin (RT), a Category B biothreat agent, provokes an acute respiratory distress syndrome marked by pro-inflammatory cytokine and chemokine production, neutrophilic exudate, pulmonary edema. The severity RT exposure is attributed to the tropism toxin’s subunit (RTB) for alveolar macrophages airway epithelial cells, coupled with extraordinarily potent ribosome-inactivating properties enzymatic (RTA). While there are currently no vaccines or treatments approved...
First-generation adenovectors have been developed for gene therapy and vaccine applications. The construction of these has entailed the use numerous types expression cassettes. It long known that first-generation can be rescued more easily to higher titers with some transgenes than others. This study systematically shown there marked differences in growth properties recombinant attributable promoters, orientation transgene within E1A/E1B-deleted region, inclusion E3 region. In addition, we...
The aim of this study is to investigate the capability an apoA-I mimetic with multiple amphipathic helices form HDL-like particles in vitro and vivo. To generate multivalent track peptide mimetic, we have constructed a peptibody by fusing two tandem repeats 4F C terminus murine IgG Fc fragment. resultant peptidbody, mFc-2X4F, dose-dependently promoted cholesterol efflux vitro, potency was superior monomeric peptide. Like apoA-I, mFc-2X4F stabilized ABCA1 J774A.1 THP1 cells. formed larger HDL...
Abstract Background Angiogenesis is a driver of platinum resistance in ovarian cancer. We assessed the effect combination pazopanib and paclitaxel followed by maintenance patients with platinum-resistant/refractory Integrins α v β 3 5 are both upregulated tumor-associated vasculature. [ 18 F]Fluciclatide novel PET tracer that has high affinity for integrins 3/5 , was used to assess anti-angiogenic pazopanib. Patients methods conducted an open-label, phase Ib study received 1 week...
Abstract Background Patient-derived xenograft (PDX) mouse tumour models can predict response to therapy in patients. Predictions made from PDX cultures (PDXC) would allow for more rapid and comprehensive evaluation of potential treatment options patients, including drug combinations. Methods We developed a library BRAF-mutant metastatic melanoma, high-throughput drug-screening (HTDS) platform utilising clinically relevant exposures. then evaluated 34 antitumor agents across eight melanoma...