A5034088296- Extracellular vesicles in disease
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Nanoplatforms for cancer theranostics
- Advanced Biosensing Techniques and Applications
- Circular RNAs in diseases
- Silk-based biomaterials and applications
- Insect Utilization and Effects
- Photoacoustic and Ultrasonic Imaging
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Nanoparticle-Based Drug Delivery
- Insect Pest Control Strategies
- Silkworms and Sericulture Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- DNA and Nucleic Acid Chemistry
- Gene expression and cancer classification
- IL-33, ST2, and ILC Pathways
- Osteoarthritis Treatment and Mechanisms
- Transgenic Plants and Applications
Incheon National University
2016-2025
Georgia Institute of Technology
2008-2021
Emory University
2008-2021
Bioengineering Center
2020
Incheon Medical Center
2020
Government of the Republic of Korea
2020
Seoul National University
2000-2009
Intracellular pH (pHi) plays a critical role in the physiological and pathophysiological processes of cells, fluorescence imaging using pH-sensitive indicators provides powerful tool to assess pHi intact cells subcellular compartments. Here we describe nanoparticle-based ratiometric sensor, comprising bright photostable semiconductor quantum dot (QD) fluorescent proteins (FPs), exhibiting dramatically improved sensitivity photostability compared BCECF, most widely used dye for imaging. We...
Stem cells and their paracrine factors have emerged as a resource for regenerative medicine. Many studies shown the beneficial effects of secreted from adult stem cells, such exosomes, on skin aging. However, to date, few reports demonstrated use exosomes derived human pluripotent treatment In this study, we collected conditioned medium induced (iPSCs) investigated effect aged dermal fibroblasts (HDFs). Cell proliferation viability were determined by an MTT assay cell migration capacity was...
There are extensive studies on the applications of extracellular vesicles (EVs) produced in cell culture for therapeutic drug development. However, large quantities EVs needed
Non-small cell lung cancer (NSCLC) is one of the leading causes death from worldwide. The delivery and controlled regulation miRNAs via exosomes known as a potential therapeutic approach in treatment cancer. In this study, human cell-derived were used vehicles for miRNAs, we investigated their anti-tumor anti-angiogenic effects on NSCLCs that cultured 2D 3D microfluidic devices. We demonstrated contained miRNA-497 (miR-497) effectively suppressed tumor growth expression associated genes,...
Sonodynamic therapy (SDT), wherein sonosensitizers irradiated with ultrasound (US) produce cytotoxic reactive oxygen species (ROS), has garnered great attention as a promising alternative to photodynamic owing the significantly increased depth of tissue penetration. The development nanocarriers that can selectively deposit into tumor tissues without systemic toxicity is crucial facilitate translation SDT clinical use. In this study, exosomes, class naturally occurring nanoparticles, were...
Oxidative stress is implicated in many diseases, including cardiovascular and neurodegenerative diseases. Because an increased level of oxidative causes apoptosis, it necessary to inhibit cellular responses stress. In this study, Carex, a nanovesicle from carrot, was isolated investigated as novel biomaterial with antioxidative function cardiomyoblasts neuroblastoma cells. A high concentration nanovesicles purified carrots, using size-exclusion chromatography combination ultrafiltration. The...
Sonodynamic therapy has shown promise as an effective alternative to conventional photodynamic owing its ability treat deep-seated tumors.However, the development of stimuli-responsive sonosensitizers with high biocompatibility faces a significant challenge.Methods: In this study, we developed dual desirable biosafety using extracellular vesicles (EVs), class naturally occurring nanoparticles.Indocyanine green (ICG), which functions both sonosensitizer and photoacoustic (PA) imaging agent,...
Glioblastoma (GBM) is the most aggressive malignant brain tumor and has a high mortality rate. Photodynamic therapy (PDT) emerged as promising approach for treatment of tumors. However, use PDT GBM been limited by its low blood‒brain barrier (BBB) permeability lack cancer-targeting ability. Herein, endothelial cell-derived extracellular vesicles (bEVs) were used biocompatible nanoplatform to transport photosensitizers into tumors across BBB. To enhance efficacy, photosensitizer chlorin e6...
Abstract Extracellular vesicles (EVs) are nano‐sized surrounded by a lipid bilayer containing bioactive materials, including proteins, nucleic acids, lipids, and metabolites. They function as messengers secreted cells, playing crucial role in cell‐to‐cell communication. Plant‐derived extracellular (P‐EVs), plant being investigated therapeutic agents drug delivery carriers due to their high biocompatibility, productivity, effective cellular delivery, exerting effects through physiological...
Prostate cancer is the fifth leading cause of cancer-related deaths among males worldwide. However, biomarker for diagnosing prostate that used currently has limitations must be overcome. Recently, several studies have demonstrated liquid biopsy can implemented by using exosome miRNAs. current methods detection miRNAs are time-consuming, expensive, and laborious. Thus, we investigated a novel method involves use molecular beacons in situ exosomes from cells. We chose miRNA-375 miRNA-574-3p...
Background: Glioblastoma (GBM) is one of the most aggressive types brain cancer. GBM progression closely associated with microglia activation; therefore, understanding regulation crosstalk between human and may help develop effective therapeutic strategies. Elucidation efficient delivery microRNA (miRNA) via extracellular vesicles (EVs) their intracellular communications required for applications in treatment. Methods: We used cells (U373MG) microglia. MiRNA-124 was loaded into...
Abstract Extracellular vesicles (EVs) contain useful biomarkers for disease diagnosis and are promising biomaterials the delivery of therapeutic molecules in vivo . Accordingly, an efficient concentration method is necessary large‐scale production or high‐throughput isolation EVs from bulk liquid samples, including culture medium body fluids, to achieve their clinical application. However, current EV methods, ultrafiltration, limited with respect cost, efficiency, centrifugation time. In...
An important aspect of vascular biology is the identification regulators stress-sensitive genes that play critical roles in mediating inflammatory response. Here, we show expression HuR human umbilical vein endothelial cells regulated by shear stress and statin treatment; HuR, turn, regulates other such as Kruppel-like factor 2 (Klf2), nitric oxide synthase (eNOS), bone morphogenic protein 4 (BMP-4). We found siRNA knockdown HuR-inhibited responses cells, including ICAM-1 VCAM-1...
The ability to visualize mRNA in single living cells and monitor real-time the changes of level localization can provide unprecedented opportunities for biological disease studies.However, detection specificity sensitivity are critically dependent on selection target sequences their accessibility.We carried out an extensive study accessibility BMP-4 using 10 different designs molecular beacons (MBs), identified optimal beacon design.Specifically, MB design 1 8 (MB1 MB8), fluorescent...