A5074472963- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Ocular Disorders and Treatments
- Wound Healing and Treatments
- Retinopathy of Prematurity Studies
- Angiogenesis and VEGF in Cancer
- Advanced Glycation End Products research
- Neuropeptides and Animal Physiology
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- PARP inhibition in cancer therapy
- Toxoplasma gondii Research Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Hemostasis and retained surgical items
- Connexins and lens biology
- Skin Protection and Aging
- Photochromic and Fluorescence Chemistry
- Nerve injury and regeneration
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Redox biology and oxidative stress
- Cell death mechanisms and regulation
- Developmental Biology and Gene Regulation
Shandong First Medical University
2014-2024
Shandong Eye Hospital
2014-2024
Shandong Eye Institute & Hospital
2014-2019
Sun Yat-sen University
2009-2014
Umeå University
2013
Substance P (SP) is a neuropeptide, predominantly released from sensory nerve fibers, with potentially protective role in diabetic corneal epithelial wound healing. However, the molecular mechanism remains unclear. We investigated of SP against hyperglycemia-induced healing defects, using type 1 mice and high glucose–treated cells. Hyperglycemia induced delayed healing, accompanied by attenuated sensation, mitochondrial dysfunction, impairments Akt, epidermal growth factor receptor (EGFR),...
To explore the role and mechanism of bone marrow-derived mesenchymal stem cells (BM-MSCs) in corneal epithelial wound healing type 1 diabetic mice.Diabetic mice were treated with subconjunctival injections BM-MSCs or recombinant tumor necrosis factor-α-stimulated gene/protein-6 (TSG-6). The rate was examined by fluorescein staining. mRNA protein expression levels TSG-6 measured quantitative RT-PCR Western blot. infiltrations leukocytes macrophages analyzed flow cytometry immunofluoresence...
Keratoconus is a common ectatic corneal disorder in adolescents and young adults that can lead to progressive visual impairment or even legal blindness. Despite the high prevalence, its etiology not fully understood. In this study, we performed single-cell RNA sequencing (scRNA-Seq) analysis on 39,214 cells from central corneas of patients with keratoconus healthy individuals, define involvement each cell type during disease progression. We confirmed role stromal disease, where dysregulation...
Abstract Ciliary neurotrophic factor (CNTF), a well-known neuroprotective cytokine, has been found to play an important role in neurogenesis and functional regulations of neural stem cells. As one the most innervated tissue, however, CNTF cornea epithelium remains unclear. This study was explore roles mechanisms activation corneal epithelial stem/progenitor cells wound healing both normal diabetic mouse epithelium. In mice subjecting mechanical removal epithelium, cell were promoted by...
Human pluripotent stem cells (hPSCs) hold great promise for the treatment of various human diseases. However, their therapeutic benefits and mechanisms treating corneal endothelial dysfunction remain undefined. Here, we developed a regimen consisting combination hPSC-derived precursors (CEPs) with nicotinamide (NAM) effective dysfunction. In rabbit nonhuman primate models, intracameral injection CEPs NAM achieved long-term recovery clarity thickness, similar outcome cultured (CECs). The...
The corneal endothelium is critical for maintaining clarity by mediating hydration through barrier and pump functions. Progressive loss of endothelial cells during aging has been associated with the development Fuchs dystrophy (FECD), one main causes cornea-related vision loss. mechanisms underlying FECD remain elusive. Single-cell RNA sequencing isolated healthy human corneas discovered 4 subpopulations distinctive signatures. Unsupervised clustering analysis uncovered nuclear enriched...
Purpose Macrophages have been shown to play a critical role in the wound healing process. In present study, of macrophages after autologous corneal transplantation was investigated by depleting local infiltrated macrophages. Methods Autologous model used induce repair Balb/c mice. were depleted sub-conjunctival injections clodronate-containing liposomes (Cl2MDP-LIP). The presence CD11b+ F4/80+ macrophages, α-smooth muscle actin+ (α-SMA+) myofibroblasts, CD31+ vascular endothelial cells and...
The therapeutic effects of allogeneic cultivated limbal epithelial transplantation (CLET) for symblephara at different degrees caused by ocular burns were evaluated in this study.A series interventional cases involved retrospective study. Eighty eyes (80 patients) with underwent CLET and the success rates surgical treatment as well corneal conditions risk factors recurrent symblepharon analyzed.The average age patients was 32.4 ± 13.7 years (ranged from 4 to 60 years). follow-up time 26.4...
Excessive exposure of the eye to ultraviolet B light (UVB) leads corneal edema and opacification because apoptosis endothelium. Our previous study found that nicotinamide (NIC), precursor adenine dinucleotide (NAD), could inhibit endothelial-mesenchymal transition accelerate healing wound endothelium in rabbit. Here we hypothesize NIC may possess capacity protect cornea from UVB-induced endothelial apoptosis. Therefore, a mouse model cultured cell were used examine effect NAD+ precursors,...
Trichostatin A (TSA) has been shown to prevent fibrosis in vitro and vivo. The present study aimed at investigating the role of reactive oxygen species (ROS) scavenging by TSA on transforming growth factor-<i>β</i> (TGF-<i>β</i>)-induced myofibroblast differentiation corneal fibroblasts vitro. Human immortalized were treated with TGF-<i>β</i> presence TSA, NAD(P)H oxidase inhibitor diphenyleneiodonium (DPI), antioxidant <i>N</i>-acetyl-cysteine (NAC), NF-E2–related factor 2-antioxidant...
Abstract Background The corneal endothelium maintains hydration through the barrier and pump function, while its dysfunction may cause edema vision reduction. Considering development from neural crest cells (NCCs), here we investigated efficacy of human induced pluripotent stem cell (hiPSC)-derived NCCs for endothelial regeneration in rabbits. Methods Directed differentiation hiPSC-derived was achieved using chemically defined medium containing GSK-3 inhibitor TGF-β inhibitor. differentiated...
Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 has been shown to increase proliferative capacity and even immortalize primary keratinocytes. Here, we demonstrate that rabbit limbal epithelial cells (LECs) treated with also exhibited improved colony-forming efficiency by enhancing the expansion of stem/progenitor cells. Moreover, treatment rapid adherence in initial inoculation In addition, elevated intracellular glutathione level decreased cellular reactive oxygen species (ROS)...
To construct an auto-tissue-engineered lamellar cornea (ATELC) for transplantation, based on acellular porcine corneal stroma and autologous limbal explants, a dynamic culture process, which composed of submersion culture, perfusion air-liquid interface was performed using appropriate parameters. The results showed that the ATELC-Dynamic possessed histological structure DNA content were similar to native (NLC, p>0.05). Compared NLC, protein contents zonula occludens-1, desmocollin-2 integrin...
Glaucoma is a serious eye disease that can lead to loss of vision. Unfortunately, effective treatments are limited by poor bioavailability antiglaucoma medicine due short residence time on the preocular surface.To solve this, we successfully prepared novel controlled-release ion-exchange microparticles deliver betaxolol hydrochloride (BH). Montmorillonite/BH complex (Mt-BH) was acidification-intercalation, and this encapsulated in microspheres (Mt-BH [BMEMs]) oil-in-oil emulsion-solvent...
Abstract Chronic inflammation and severe dry eye are two important adverse factors for the successful transplant of cultured limbal stem cells. The aim this study was to investigate effects hyperosmotic stress (a key pathological factor in eye) on corneal epithelial cells (CESCs) wound healing. We observed that CESCs exhibited significant morphological changes when treated with interleukin-1 beta (IL-1β), tumor necrosis alpha (TNF-α), or stress. Colony-forming efficiency colony-forming size...
Induction of autologous stem cells for directed differentiation has become a predominant method to obtain tissue reconstruction. However, the low inducing efficiency and contamination with other type hinder its clinical utilization. Here we report novel phenomenon that corneal epithelial maintain long-term proliferative capacity tissue-specific cell phenotype by factors secreted from murine embryonic (ESCs). The rabbit grew very well in culture medium addition 40% ESC conditioned (ESC-CM)....
Eye is a complex organ with highly specialized tissue structure. The establishment of human pluripotent stem cells (hPSCs) has allowed the simulation eye development in vitro. Most differentiation works hPSC-derived ocular focus on single, tissue-specific lineage, however, that faces difficulty reflecting complexity development. Recently, generation self-formed ectodermal autonomous multi-zone availably mimics process whole-eye In this study, we developed rapid defined method to induce...
Corneal endothelium is a single layer of hexagonal cells that maintains the corneal transparency and thickness through its barrier pump function. For treatment endothelial dysfunction, transplantation tissue-engineered direct injection cultured were developed because severe shortage donor cornea worldwide. However, technique difficulty or safety risk still remained. In this study, we report novel mini-sheet for cell compare with effects single-cell in rabbit model. Compared reported...