A5030021840- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Traditional Chinese Medicine Analysis
- Viral Infections and Immunology Research
- Fungal Biology and Applications
- Transgenic Plants and Applications
- Neurological Disease Mechanisms and Treatments
- Animal Virus Infections Studies
- Biochemical and Structural Characterization
- Phytochemistry and Bioactive Compounds
- Entomopathogenic Microorganisms in Pest Control
- Sesame and Sesamin Research
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Medicinal Plants and Bioactive Compounds
- Natural product bioactivities and synthesis
- Antimicrobial Peptides and Activities
- Insect and Pesticide Research
- Alzheimer's disease research and treatments
- Phytochemical Studies and Bioactivities
- Tea Polyphenols and Effects
- Sunflower and Safflower Cultivation
- Curcumin's Biomedical Applications
- Ion Channels and Receptors
- Redox biology and oxidative stress
China Medical University
2009-2019
China Medical University
2010-2011
Taiwan Agricultural Chemicals and Toxic Substances Research Institute
2003-2007
National Chung Hsing University
2005-2007
Council of Agriculture
2003
K. pneumoniae is the predominant pathogen isolated from liver abscesses of diabetic patients in Asian countries. Although elevated blood glucose levels cause various immune problems, its effects on virulence are unknown. This study investigated regulation capsular polysaccharide (CPS) biosynthesis, a major determinant for virulence, response to exogenous glucose. We found that produce more CPS glucose-rich medium via reduction cyclic AMP (cAMP) levels. Individual deletion cyaA or crp, which...
Although enterovirus 71 (EV71) is an important public health threat, especially in the Asia-Pacific region, there are still no effective drugs or vaccines to treat and prevent EV71 infection. Therefore, it critical develop prophylactic therapeutic agents against EV71. Rosmarinic acid (RA), a phytochemical, has been discovered possess broad spectrum of biological activities. The virucidal effects RA on were determined by MTT, western blot, median cell culture infectious dose, apoptosis...
Sesamin and sesamolin, abundant lignans found in sesame oil, have been demonstrated to possess several bioactivities beneficial for human health. Excess generation of nitric oxide lipopolysaccharide-stimulated rat primary microglia cells was significantly attenuated when they were pretreated with sesamin or sesamolin. The neuroprotective effect sesamolin also observed vivo using gerbils subjected a focal cerebral ischemia induced by occlusion the right common carotid artery middle artery....
IscR, an Fe–S cluster-containing transcriptional factor, regulates genes involved in various cellular processes. In response to environmental stimuli such as oxidative stress and iron levels, IscR switches between its holo apo forms regulate targets. binding sequences are classified into two types: the type 1 box that is specific for holo-IscR binding, 2 binds holo- apo-IscR. Studying Klebsiella pneumoniae CG43S3, we have previously shown availability capsular polysaccharide (CPS)...
The mushrooms of diverse Lingzhi species have been traditionally consumed as luxurious functional food supplements in Chinese society. FIP-gts, a fungal immunomodulatory protein found Song-Shan (Ganodera tsugae) has proposed to possess therapeutic effects on cancer and autoimmune diseases. To produce active FIP-gts for evaluation oral administration, recombinant (rFIP-gts) fused with 6His-tag at its C-terminus was expressed Sf21 insect cells by the baculovirus expression system. High yield...
A bi-cistronic baculovirus-insect/larval system containing a polyhedron promoter, an internal ribosome entry site (IRES), and egfp gene was developed as cost-effective platform for the production of recombinant human interferon gamma (rhIFN-γ). There no significant difference between amounts rhIFN-γ produced in baculovirus-infected Spodoptera frugiferda 21 cells grown serum-free medium serum-supplemented medium, while Trichoplusia ni (T. ni) exigua (S. exigua) larvae afforded amounting to...
Klebsiella pneumoniae is the predominant pathogen isolated from liver abscess of diabetic patients in Asian countries. With spread multiple-drug-resistant K. pneumoniae, there an increasing need for development alternative bactericides and approaches to block production bacterial virulence factors. Capsular polysaccharide (CPS), especially K1 K2 serotypes, considered major determinant virulence. We found that extracts traditional Chinese medicine Fructus mume inhibited growth strains both...
Oxygen-base (O-base) oxidation in protein backbone is important the fragmentation due to attack from reactive oxygen species (ROS). In this study, an alanine peptide was used model system investigate O-base by employing density functional theory (DFT) calculations combining with continuum solvent model. Detailed reaction steps were analyzed along their rate constants. Most of reactions for are exothermic except bond-breakage Cα-N bond form hydroperoxy radical. Among investigated activated...
The inhibition of Na + /K -ATPase by versatile steroid-like compounds contributes to the putative therapeutic effects many Chinese medicinal cardiac products via same molecular mechanism triggered glycosides. Five major compounds, antcin A, B, C, H, and K were isolated from Niuchangchih (Antrodia camphorata), a unique Taiwan mushroom, all inhibited -ATPase. Antcin A exhibited significantly higher inhibitory potency than other four antcins, though weaker ginsenoside Rh2 . In contrast,...
The maturation of mastoparan B, the major toxin peptide in venom Vespa basalis, requires enzymatic cleavage its prosequence presumably via sequential liberation dipeptides. putative processing enzyme, dipeptidyl peptidase IV, was expressed as a glycosylated His-tag fusion protein (rDPP-IV) baculovirus expression system. rDPP-IV purified by one-step nickel-affinity chromatography verified Western blot and LC-MS/MS analysis. k(cat)/K(m) determined to be range 10-500 mM(-1)·S(-1) for five...
Abstract The baculovirus–insect expression system is a popular tool for recombinant protein production. standard method infecting insect larvae with baculovirus production involves either feeding occlusion bodies or injecting budded virus into the cuticle. In this study, we showed that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) at titers >10 8 pfu/mL efficiently infected Trichoplusia ni ( T. ) through aerosol inoculation of pressure 5.5 × 10 4 Pa. dipping in...
To develop an economical, easy technique for producing recombinant E2 glycoprotein (rE2) of classical swine fever virus (CSFV) as a candidate immunogen, bi-cistronic baculovirus/larvae expression vector was constructed using p10 promoter, internal ribosome entry site, and the gfp gene. Trichoplusia ni larvae were successfully infected with occluded baculovirus via feed, characteristics rE2 confirmed by immunoblot glycosylation stain. at concentration 0.6-0.8 mg/ml without degradation...