A5072040147- Alzheimer's disease research and treatments
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Neurological Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Systemic Lupus Erythematosus Research
- Cancer-related molecular mechanisms research
- Viral Infections and Immunology Research
- Colorectal Cancer Screening and Detection
- Microbial Natural Products and Biosynthesis
- Chromium effects and bioremediation
- Reproductive System and Pregnancy
- Liver physiology and pathology
- Cell death mechanisms and regulation
Weifang People's Hospital
2024
Weifang Medical University
2021-2023
China Medical University
2019-2022
Dalian Medical University
2012-2016
Xiamen University
2011
Dalhousie University
2004-2009
Gut microbiota imbalance is found in fecal samples from DN patients, which Roseburia intestinalis significantly decreased, while Bacteroides stercoris increased. There a significant correlation between gut and clinical indexes related to lipid metabolism, glucose renal function. The may be predictive factors for the development progression of DN, although further studies are warranted illustrate their regulatory mechanisms.
Accumulating studies have indicated that the gut microbiota plays a pivotal role in onset of autoimmune diseases by engaging complex interactions with host. This review aims to provide comprehensive overview existing literatures concerning relationship between and diseases, shedding light on interplay microbiota, host immune system. Furthermore, we aim summarize impacts potential mechanisms underlie primarily focusing systemic lupus erythematosus, rheumatoid arthritis, Sjögren’s syndrome,...
Previous studies have implicated that the transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) effectively alleviates systemic lupus erythematosus (SLE) primarily due to immunomodulatory effects. However, little is known about role hUC-MSC-derived exosomes in SLE. This study carried out investigate modifying effects hUC-MSC-exosomes on differentiation and function immune were extracted from cultural supernatant hUC-MSCs by ultrahigh speed centrifugation. Quantitative...
Inteins catalyze protein splicing in a fashion similar to how self-splicing introns RNA splicing. Split-inteins precise ligation of two separate polypeptides through trans-splicing highly specific manner. Here we report method using circumvent the packaging size limit gene therapy vectors. To demonstrate this method, chose large dystrophin and an adeno-associated viral (AAV) vector, which has small size. A functional 6.3-kb Becker-form cDNA was broken into pieces modified by adding...
Accumulating studies have implicated that circular RNAs (circRNAs) play vital roles in the pathogenesis of rheumatoid arthritis (RA). Dysregulation macrophage polarization leads to immune homeostatic imbalance RA. However, altering effects and mechanisms circRNAs on macrophages balance remain largely unclear. We aimed investigate potential role circRNA_17725 The high-throughput sequence was performed identify dysregulated confirmed data by CCK-8, EdU, Annexin V/PI staining elucidate...
Inteins are protein-intervening sequences that can self-excise and concomitantly splice together the flanking polypeptides. Two-piece split inteins capable of protein trans-splicing have been found in nature engineered laboratories, but they all a similar site corresponding to endonuclease domain intein. Can be at other sites do trans-splicing? After testing 13 into Ssp DnaB mini-intein, we report finding three new each produced two-piece intein trans-splicing. These functional located...
Intein-based protein cleavages, if carried out in a controllable way, can be useful tools of recombinant purification, ligation, and cyclization. However, existing methods using contiguous inteins were often complicated by spontaneous which could severely reduce the yield desired product. Here we demonstrate new method cleavages without any cleavage, an artificial S1 split-intein consisting 11-aa N-intein (I(N)) 144-aa C-intein (I(C)). In C-cleavage design, I(C) sequence was embedded...
Lanthanum (La) is a kind of rare earth element (REE) widely found in nature. La has neurotoxicity and can impair learning memory, but the underlying mechanism still not completely clear. The mitochondrial calcium uniporter (MCU) complex cause uptake cytoplasmic ([Ca2+]c) into mitochondria thereby resist [Ca2+]c overload. However, abnormal increase matrix ([Ca2+]m) also disturb fission-fusion balance, then induce excessive mitophagy, disrupt quality control (MQC). It unclear whether interfere...
An apoptosis correlated molecule—protein Latcripin-1 of Lentinula edodes C91-3—was expressed and characterized in Pichia pastoris GS115. The total RNA was obtained from C91–3. According to the transcriptome, full-length gene isolated with 3'-Full Rapid Amplification cDNA Ends (RACE) 5'-Full RACE methods. inserted into secretory expression vector pPIC9K. protein GS115 analyzed by Sodium Dodecylsulfonate Polyacrylate Gel Electrophoresis (SDS-PAGE) Western blot. blot showed that successfully....
Introduction Accumulating evidence has supported that gut microbiota and metabolite profiles play indispensable roles in the pathogenesis of colorectal cancer (CRC), which ranks as third most common second leading cause cancer-related deaths worldwide. However, alterations tumoral or circulating microbiomes CRC remain incompletely understood. It been well-documented tissue serum with low microbial biomass could be screened by use 2bRAD sequencing for microbiome (2bRAD-M) at species...
Objectives n‐Hexane, a common industrial organic solvent, causes multiple organ damage, especially neurotoxicity, which is proved to be caused by its metabolite 2,5‐hexanedione (2,5‐HD). We previously showed that 2,5‐HD induced apoptosis of rat bone marrow mesenchymal stem cells (BMSCs) . In the current study, we explored mechanism 2,5‐HDinduced apoptosis, role played reactive oxygen species (ROS) Methods Intracellular ROS levels after treatment were measured dichloro‐dihydro‐fluorescein...
Abstract Rare earth elements (REEs) have caused bioaccumulation and adverse health effects attributed to extensive application. The penetrability of REEs across the blood–brain barrier (BBB) contributes their neurotoxicity process, but potential mechanisms affecting BBB integrity are still obscure. present study was designed investigate lanthanum on adheren junctions actin cytoskeleton in vitro using bEnd.3 cells. After chloride (LaCl3, 0.125, 0.25 0.5 mM) treatment, cytotoxicity against...
To investigate the effect of Angelicae Pubescentis Radix (APR) on activity endocannabinoid hydrolase and N-acylethanolamine-hydrolyzing acid amidase (NAAA), to demonstrate mechanism anti-inflammatory APR by in vitro lipopolysaccharide (LPS)-induced inflammation model.APR essential oil was extracted steam distillation, chemical components were identified GC-MS. Enzymatic performed using recombinant NAAA-overexpressing protein detected LC-MS. Lipids methonal/chloroform mixure analyzed mRNA...
4126 Background: For patients (pts) with hepatocellular carcinoma (HCC) who are not suitable for surgical resection, orthotopic liver transplantation (OLT) is the only potential curative maneuver. Adjuvant chemotherapy may be administered to those pts high risk of recurrence based on anecdotal analysis improving outcome. The presence microvascular invasion, multifocality ≥3 tumor nodules, tumor’s size ≥ 5 cm, and poor differentiation considered as factors HCC recurrence. A retrospective was...