A5044449102- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- COVID-19 Impact on Reproduction
- Reproductive System and Pregnancy
- Pneumocystis jirovecii pneumonia detection and treatment
- Chemical Safety and Risk Management
- Infection Control and Ventilation
- HIV-related health complications and treatments
- Pharmacological Effects and Toxicity Studies
- Advanced biosensing and bioanalysis techniques
- Blood groups and transfusion
- Particle Dynamics in Fluid Flows
- Cerebral Palsy and Movement Disorders
- Immune responses and vaccinations
- Immune cells in cancer
- Pregnancy and preeclampsia studies
- Long-Term Effects of COVID-19
- Toxoplasma gondii Research Studies
Tulane University
2019-2025
Cytomegalovirus (CMV) is a prevalent β-herpesvirus that persists asymptomatically in immunocompetent hosts. In people with HIV-1 (PWH), CMV associated persistence of the reservoir and particular inflammatory related co-morbidities. The true causative role HIV-associated pathologies remains unclear given nearly all PWH are coinfected CMV. this study, we examined acute phase SIV dynamics cohorts rhesus macaques were seropositive or -negative for (RhCMV). We observed expansion CCR5+ target CD4+...
Congenital cytomegalovirus (cCMV) is the leading infectious cause of birth defects worldwide, yet immune determinants protection to inform design a maternal vaccine remain elusive. Here, we characterized outcome primary rhesus CMV (RhCMV) infection during pregnancy in an competent nonhuman primate (NHP) model. RhCMV DNA was detected amniotic fluid and/or fetal tissues six 12 (50% placental transmission) CMV-naive macaque dams inoculated intravenously with early second trimester gestation....
Abstract Human cytomegalovirus (HCMV) encodes four viral Fc-gamma receptors (vFcγRs) that counteract antibody-mediated activation in vitro, but their role infection and pathogenesis is unknown. To examine vivo function an animal model evolutionarily closely related to humans, we identified characterized Rh05, Rh152/151 Rh173 as the complete set of vFcγRs encoded by rhesus CMV (RhCMV). Each one these proteins displays functional similarities prospective HCMV orthologs with respect...
Congenital cytomegalovirus (cCMV) is the leading infectious cause of neonatal neurological impairment worldwide, but viral factors enabling vertical spread across placenta remain undetermined. The pentameric complex (PC), composed subunits gH/gL/UL128/UL130/UL131A, has been demonstrated to be important for entry into nonfibroblast cells in vitro. These findings link PC broad cell tropism and virus dissemination vivo, denoting all as potential targets intervention strategies vaccine...
High concentrations of aerosols can be generated within the sort collection area cell sorters during instrument failures that cause stream to deviate, such as a partial nozzle obstruction. Complete containment these aerosol particles becomes essential for operator safety when working with potentially infectious or hazardous samples. Currently, is accomplished through generation continuous negative airflow using an evacuation system, which enhanced by primary devices biosafety cabinets....
<title>Abstract</title> Congenital cytomegalovirus (cCMV) is the leading infectious cause of birth defects worldwide, yet immune determinants protection to inform maternal vaccine design remain elusive due lack a translational animal model. Here, we characterized outcome primary rhesus CMV (RhCMV) infection in pregnant, immunocompetent, CMV-naïve macaques. RhCMV DNA was detected amniotic fluid and/or fetal tissues six 12 (50% placental transmission) dams following early second trimester...
Cytomegalovirus (CMV) is the most common congenital infection and cause of birth defects worldwide. Primary CMV during pregnancy leads to a higher frequency (cCMV) than maternal re-infection, suggesting that immunity confers partial protection. However, poorly understood immune correlates protection against placental transmission contributes current lack an approved vaccine prevent cCMV. In this study, we characterized kinetics plasma rhesus (RhCMV) viral load (VL) RhCMV-specific antibody...
Abstract Human cytomegalovirus (HCMV) encodes four viral Fc-gamma receptors (vFcγRs) that counteract antibody-mediated activation in vitro , but their role infection and pathogenesis is unknown. To examine the vivo function of vFcγRs animal hosts closely related to humans, we identified characterized encoded by rhesus CMV (RhCMV). We demonstrate Rh05, Rh152/151 Rh173 represent complete set RhCMV vFcγRs, each displaying functional similarities respective HCMV orthologs with respect...
Cytomegaloviruses (CMVs) are highly adapted to their host species resulting in strict specificity. Hence, vivo examination of all aspects CMV biology employs animal models using host-specific CMVs. Infection rhesus macaques (RM) with (RhCMV) has been established as a representative model for infection humans HCMV due the close evolutionary relationships both and virus. However, only available RhCMV clone that permits genetic modifications is based on 68–1 strain which passaged fibroblasts...
Recent functional, gene expression, and epigenetic studies have suggested the presence of a subset mature natural killer (NK) cells responsible for maintaining NK cell memory. The lack endogenous clonal markers in impedes understanding genesis these populations. In humans, primates, mice, this phenotype memory or adaptive functions been strongly linked to cytomegalovirus related herpes virus infections. We used transplantation lentivirally-barcoded autologous hematopoietic stem progenitor...
The maternal decidua is an immunologically complex environment that balances maintenance of immune tolerance to fetal paternal antigens with protection the fetus against vertical transmission pathogens. To better understand host determinants congenital infection at maternal-fetal tissue interface, we performed a comparative analysis innate and adaptive cell subsets in peripheral blood healthy rhesus macaque pregnancies across all trimesters gestation determined changes after Zika virus...
Approximately 0.7% of infants are born with congenital cytomegalovirus (CMV), making it the most common infection. About 1 in 5 congenitally infected babies will suffer long-term sequelae, including sensorineural deafness, intellectual disability, and epilepsy. CMV infection is highly species-dependent, rhesus (RhCMV) monkey fetuses only animal model that replicates essential features (cCMV) humans, placental transmission, fetal disease, loss. Using experimental data from RhCMV seronegative...
Congenital cytomegalovirus (cCMV) is the leading infectious cause of neurologic defects in newborns with particularly severe sequelae setting primary CMV infection first trimester pregnancy. The majority cCMV cases worldwide occur after non-primary CMV-seropositive women; yet extent to which pre-existing natural CMV-specific immunity protects against reinfection or reactivation during pregnancy remains ill-defined. We previously reported on a novel nonhuman primate model rhesus macaques...
Human immunodeficiency virus (HIV) and malaria, caused by infection with Plasmodium spp., are endemic in similar geographical locations. As a result, there is high potential for HIV/Plasmodium co-infection, which increases the pathology of both diseases. However, immunological mechanisms underlying exacerbated disease observed co-infected individuals poorly understood. Here, we used rhesus macaque (RM) model to characterize immunopathogenic impact fragile co-infection during antiretroviral...
Human immunodeficiency virus (HIV) and malaria, caused by infection with Plasmodium spp., are endemic in similar geographical locations. As a result, there is high potential for HIV/Plasmodium co-infection, which increases the pathology of both diseases. However, immunological mechanisms underlying exacerbated disease observed co-infected individuals poorly understood. Moreover, limited data available on impact co-infection antiretroviral (ART)-treated HIV infection. Here, we used rhesus...
Abstract Approximately 1 in 200 infants is born with congenital cytomegalovirus (CMV), making it the most common infection. About 5 congenitally-infected babies will suffer long-term sequelae, including sensorineural deafness, intellectual disability, and epilepsy. CMV infection highly species-dependent, Rhesus (RhCMV) of rhesus monkey fetuses only animal model that replicates essential features humans, placental transmission, fetal disease, loss. To better understand determinants dynamics...
Abstract Cytomegaloviruses (CMVs) are highly adapted to their host species resulting in strict specificity. Hence, vivo examination of all aspects CMV biology employs animal models using host-specific CMVs. Infection rhesus macaques (RM) with (RhCMV) has been established as a representative model for infection humans HCMV due the close evolutionary relationships both and virus. However, commonly used 68-1 strain RhCMV passaged fibroblasts decades multiple genomic changes tissue culture...
Abstract Congenital cytomegalovirus (cCMV) is the leading infectious cause of neurologic defects in newborns with particularly severe sequelae setting primary CMV infection first trimester pregnancy. The majority cCMV cases worldwide occur after non-primary CMV-seropositive women; yet extent to which pre-existing natural CMV-specific immunity protects against reinfection or reactivation during pregnancy remains ill-defined. We previously reported on a novel nonhuman primate model rhesus...
Cytomegalovirus (CMV) is the most common congenital infection and cause of birth defects worldwide. Primary CMV during pregnancy leads to a higher frequency (cCMV) than maternal re-infection, suggesting that immunity confers partial protection. However, poorly understood immune correlates protection against placental transmission contributes current lack an approved vaccine prevent cCMV. In this study, we characterized kinetics plasma rhesus (RhCMV) viral load (VL) RhCMV-specific antibody...
Abstract Cytomegalovirus (CMV) is a top priority in vaccine development due to the high burden of disease immunocompromised populations and congenitally infected infants. Evidence suggests that CMV-specific Fc-mediated antibody effector responses, such as dependent cellular phagocytosis cytotoxicity, are key for prevention multiple CMV outcomes. However, human expresses three Fcγ receptors have been shown vitro synergistically interfere with anti-CMV IgG engagement host (FcγRs). We recently...
Abstract Congenital cytomegalovirus (cCMV) is the most common in utero infection and causes major neurodevelopmental deficits, but there remains no licensed vaccine to prevent cCMV. Little known about maternal immune responses that can placental CMV transmission, which could guide rational design of an effective vaccine. Using rhesus macaque (RM) model primary RM (RhCMV) during pregnancy, we established pre-existing RhCMV-neutralizing IgG protected against cCMV, even setting CD4+ T cell...