A5031418109- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- Toxoplasma gondii Research Studies
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Rabies epidemiology and control
- Cardiac Arrest and Resuscitation
- Traumatic Brain Injury and Neurovascular Disturbances
- Poxvirus research and outbreaks
- Neonatal and fetal brain pathology
- Bartonella species infections research
- Heme Oxygenase-1 and Carbon Monoxide
- Legionella and Acanthamoeba research
- Organ and Tissue Transplantation Research
- Aquaculture disease management and microbiota
- vaccines and immunoinformatics approaches
- Parasitic Infections and Diagnostics
- Cerebrospinal fluid and hydrocephalus
- T-cell and B-cell Immunology
- Poisoning and overdose treatments
- Immunodeficiency and Autoimmune Disorders
- Biosensors and Analytical Detection
- Sepsis Diagnosis and Treatment
University of Florida
2019-2024
Duke University
2018-2023
Florida College
2020-2021
UF Health Shands Hospital
2020-2021
University of Florida Health
2021
Duke Medical Center
2019
Duke University Hospital
2018
Virginia Commonwealth University
2011-2015
University of Massachusetts Chan Medical School
2006
Previous evidence suggests that a homeostatic germinal center (GC) response may limit bortezomib desensitization therapy. We evaluated the combination of costimulation blockade with in sensitized non-human primate kidney transplant model. Sensitized animals were treated bortezomib, belatacept, and anti-CD40 mAb twice weekly for month (n = 6) compared to control 7). Desensitization therapy-mediated DSA reductions approached statistical significance (P .07) significantly diminished bone marrow...
Significance Statement Although common desensitization strategies for patients with broad HLA sensitization permit transplantation via transient lowering of donor-specific antibodies, the B cell–response axis from germinal center activation to plasma cell differentiation remains intact. In this study, dual-targeting carfilzomib (a proteasome inhibitor) and belatacept costimulation blockade agent) prolonged allograft survival in highly sensitized nonhuman primates; it appears inhibit cells,...
Abstract In this study, we demonstrate complex networks of CD8 T cell cross-reactivities between influenza A virus and EBV in humans lymphocytic choriomeningitis vaccinia mice. We also show directly that cross-reactive cells mediate protective heterologous immunity Subsets populations reactive with one epitope cross-reacted either several other epitopes encoded by the same or virus. Human specific to EBV-encoded BMLF1280–288 could be two epitopes. Mouse virus-encoded a11r198–205 three...
ABSTRACT Live vaccinia virus (VV) vaccination has been highly successful in eradicating smallpox. However, the mechanisms of immunity involved mediating this protective effect are still poorly understood, and roles CD8 T-cell responses primary secondary VV infections not clearly identified. By applying concept molecular mimicry to identify potential epitopes that stimulate cross-reactive T cells specific lymphocytic choriomeningitis (LCMV) VV, we identified after screening only 115 peptides...
ABSTRACT Congenital cytomegalovirus (CMV) disease is the leading cause of permanent disability in neonates United States. Neutralizing antibodies saliva may protect against maternal CMV infection by blocking viral entry into oral epithelial cells, but antibody response to following natural not well characterized. Saliva specimens from naturally infected individuals were tested for CMV-neutralizing activity using and fibroblast cells. seronegative adults had no inherent anti-CMV activity. was...
Cytomegalovirus (CMV) causes substantial morbidity and mortality after hematopoietic stem cell transplantation (HSCT). There are limited data on risk factors for CMV viremia the safety of antiviral medications used to treat in children.We conducted a single-center retrospective study children who underwent HSCT between 2000 2016. We log-logistic regression evaluate associations clinical characteristics CMV-free survival at 100 days HSCT. compared incidences laboratory-defined adverse events...
Development of a cytomegalovirus (CMV) vaccine is high priority. However, the ability antibodies to protect against CMV infection not well characterized. Studies maternal in infants offer potential identify humoral correlates protection postnatal acquisition.This hypothesis-generating study analyzed 29 Ugandan mother-infant pairs that were followed weekly for acquisition. Seventeen mothers and no infected with human immunodeficiency virus (HIV). We evaluated association between CMV-specific...
Congenital cytomegalovirus (cCMV) is the leading infectious cause of neurologic defects in newborns with particularly severe sequelae setting primary CMV infection first trimester pregnancy. The majority cCMV cases worldwide occur after non-primary CMV-seropositive women; yet extent to which pre-existing natural CMV-specific immunity protects against reinfection or reactivation during pregnancy remains ill-defined. We previously reported on a novel nonhuman primate model rhesus macaques...
Primary amebic meningoencephalitis is a rare, usually fatal disease, caused by Naegleria fowleri. This case highlights the challenging clinicopathologic diagnosis in 13-year-old boy who swam freshwater northern Florida where previous had exposure to body of water on same property 2009.
To develop and internally validate a noninvasive method for the prediction of congenital cytomegalovirus (CMV) infection after primary maternal CMV infection.We conducted secondary analysis multicenter randomized placebo-controlled trial hyperimmune globulin to prevent infection. Women were eligible if they had infection, defined as detectable plasma CMV-specific immunoglobulin (Ig)M IgG with avidity less than 50% before 24 weeks gestation or seroconversion 28 weeks, carrying singleton fetus...
Abstract Cytomegalovirus (CMV) is the most common congenital infection and infectious cause of fetal anomaly neurologic injury. However, treatment strategies for CMV (cCMV) during pregnancy remain elusive. We report a case hydrops fetalis secondary to cCMV with minimal sequelae after maternal subsequent neonatal valganciclovir.
Cat scratch disease (CSD) typically presents as regional lymphadenopathy, following inoculation via scratch, bite, or lick to an open wound by a young cat. Annual prevalence is 22,000 cases in the United States. Although CSD self-limiting majority of cases, can manifest varying presentations and affect multiple organ systems. Serology testing for Bartonella henselae antibodies practical diagnostic tool but has limitations. Therefore, it important medical providers recognize its forms,...
Non-cholerae Vibrio species and Shewanella are organisms that rarely infect humans. Symptoms can range from gastroenteritis to wound infections septicemia. In addition, these lead multiple poor outcomes ranging amputations death. We present a case of an 11-year-old male with prepatellar bursitis the right knee due parahaemolyticus algae following open in Gulf Mexico complicated by retained oyster shell fragments. He completely recovered after removal foreign bodies organism-directed...
Background: False-negative and false-positive results in Toxoplasma serology are possible, this could be misleading. Here, we report the case of a boy with Toxocara-associated panuveitis who was initially treated for toxoplasmosis owing to immunoglobulin M (IgM) serology. Case Presentation: A nine-year-old presented intermittent headaches blurred vision left eye. Close contact domesticated animals remarkable patient’s history. Upon examination, 20/400 Slit-lamp examination revealed anterior...
Abstract PurposeTo report panuveitis due to Toxocara, with positive Toxoplasma serology. ObservationA nine-year-old boy presented a panuveitis, intermittent headaches, and visual acuity of 20/400 in his left eye. Slit lamp examination showed anterior chamber cells flare without keratic precipitates (KPs), vitreous veils, optic disc edema blurred fundus view. Initial lab values were for toxoplasmosis. Topical cycloplegic steroid eye drops, addition trimethoprim/sulfamethoxazole (TMP/SMX)...
Abstract Congenital cytomegalovirus (cCMV) is the leading infectious cause of neurologic defects in newborns with particularly severe sequelae setting primary CMV infection first trimester pregnancy. The majority cCMV cases worldwide occur after non-primary CMV-seropositive women; yet extent to which pre-existing natural CMV-specific immunity protects against reinfection or reactivation during pregnancy remains ill-defined. We previously reported on a novel nonhuman primate model rhesus...
Abstract Background Congenital cytomegalovirus (CMV) is the leading infectious cause of birth defects in United States. Development an effective CMV vaccine a public health priority. However, development limited by poor understanding immune correlates protection, including role CMV-specific IgG. Defining passively acquired maternal IgG protection half CMV-exposed, breastfeeding infants against postnatal acquisition may inform design Methods We analyzed humoral responses 29 CMV-seropositive...
Vazquez-Colon, Zasha Francheska1; Moas, Daniel1; Cardenas, Jose1; Baines, Torrey2; Pareja, Jennifer Munoz1; Collins, Shelley1; Saccoccio, Frances1; Beal, Stacy3; Schlurmann, Natalia Martinez1 Author Information
Abstract Background Procalcitonin (PCT) and c-reactive protein (CRP) have been utilized in children to assess risk for serious bacterial infections. However, there different cut-offs reported PCT CRP, which yield sensitivity specificity. This study aims compare the specificity of CRP detecting infections (SBIs), specifically urinary tract infections, bacteremia meningitis. Methods In this retrospective, single center cohort from January 2018 June 2019, we analyzed with a fever greater than...