A5040365310- Enzyme Structure and Function
- Connective tissue disorders research
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Bone Metabolism and Diseases
- Pluripotent Stem Cells Research
- Metal-Catalyzed Oxygenation Mechanisms
- Porphyrin Metabolism and Disorders
- Wound Healing and Treatments
- Advanced Proteomics Techniques and Applications
- Elasticity and Material Modeling
- Metalloenzymes and iron-sulfur proteins
- Phytase and its Applications
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Synthesis and bioactivity of alkaloids
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
Daegu Gyeongbuk Institute of Science and Technology
2013-2024
University of Wisconsin–Madison
2001-2017
Zero to Three
2013
Laboratoire de Biochimie
2013
Samsung (South Korea)
2011
Korea Advanced Institute of Science and Technology
1998
Pancreatic islets secrete hormones that play a key role in regulating blood glucose levels (glycemia). Age-dependent impairment of islet function and concomitant dysregulation glycemia are major health threats aged populations. However, the causes age-dependent decline still disputed. Here we demonstrate aging pancreatic mice humans is notably associated with inflammation fibrosis vessels but does not affect sensing insulin secretory capacity beta cells. Accordingly, when transplanted into...
The accessory protein CooC, which contains a nucleotide-binding domain (P-loop) near the N terminus, participates in maturation of nickel center carbon monoxide dehydrogenase (CODH). In this study, CooC was purified from chromatophore membranes <i>Rhodospirillum rubrum</i> with 3,464-fold purification and 0.8% recovery, its biochemical properties were characterized. is homodimer molecular mass 61–63 kDa, less than 0.1 atom Ni<sup>2+</sup> or Fe<sup>2+</sup> per dimer, has λ<sub>max</sub> at...
Various human solid tumors highly express IL-4 receptors which amplify the expression of some anti-apoptotic proteins, preventing drug-induced cancer cell death. Thus, receptor targeted drug delivery can possibly increase therapeutic efficacy in treatment. Macromolecular carriers with multivalent targeting moieties offered great advantages therapy as they not only plasma half-life but also allow drugs to cells higher specificity, minimizing deleterious effects on normal cells. In this study...
We describe a comparative study of protein production from 96 Arabidopsis thaliana open reading frames (ORFs) by cell-based and cell-free protocols. Each target was carried through four pipeline protocols used the Center for Eukaryotic Structural Genomics (CESG), one unlabeled to be in crystallization trials three 15N-labeled proteins analyzed 1H-15N NMR correlation spectroscopy. Two involved Escherichia coli two wheat germ technology. The progress each followed with all failures successes...
Abstract Elastin‐like proteins (ELPs) modeled after tropoelastin are favored in the development of biomimetic matrices due to their biocompatibility and possibility precisely control environmental responsiveness, mechanical properties, fate within cells by recombinant DNA technology‐mediated design at gene level. However, a basic prerequisite use ELPs as cell culture is presence biofunctionality that can induce adhesion‐mediated signaling pathways. To activate fibronectin‐integrin events...
Molecular changes during aging have been studied to understand the mechanism of progress. Herein, in microRNA (miRNA) expression whole blood mice were systemically reverse and propose them as non-invasive biomarkers. Through next-generation sequencing analysis, we selected 27 differentially expressed miRNAs aging. The most recognized function involved was liver steatosis, a type non-alcoholic fatty disease (NAFLD). Among miRNAs, six predicted be NAFLD, miR-16-5p, miR-17-5p, miR-21a-5p,...
Integrin-mediated interaction of neuronal cells with extracellular matrix (ECM) is important for the control cell adhesion, morphology, motility, and differentiation in both vitro vivo systems. Arg-Gly-Asp (RGD) sequence one most potent integrin-binding ligand found many native ECM proteins. An elastin-mimetic recombinant protein, TGPG[VGRGD(VGVPG)6]20WPC, referred to as [RGD-V6]20, contains multiple RGD motifs bind cell-surface integrins. This study aimed investigate how surface-adsorbed...
Abstract Poly(lactide‐ co ‐glycolide) (PLGA) and elastin‐like polypeptide (ELP) have been widely used as a biodegradable scaffold thermoresponsive matrix, respectively. However, little attention has focused on the combinatorial use of these biomaterials for tissue engineering applications. An ELP matrix TGPG[VGRGD(VGVPG) 6 ] 20 WPC (referred to REP) contains multiple Arg‐Gly‐Asp motifs. This study fabricated porous PLGA scaffolds coated with various concentration via thermally induced phase...
The expression of specific transcription factors determines the differentiated features postmitotic neurons. However, mechanism by which molecules determine neuronal cell fate and extent to functions are conserved in evolution not fully understood. In C. elegans, cholinergic peptidergic SMB sensory/inter/motor neurons innervate muscle quadrants head control amplitude sinusoidal movement. Here we show that LIM homeobox protein LIM-4 characteristics lim-4 mutant animals, terminal...
Successful islet transplantation critically depends on the isolation of healthy islets. However, procedure itself contributes to death due destruction intra- and peri-islet extracellular matrices (ECMs) during digestion. We investigated whether an RGD-containing elastin-like polypeptide (REP) could function as a self-assembling matrix replenish ECMs protects islets from cell death. Immediately following isolation, were coated with REP coacervate particles via isothermal adsorption solution...
Impaired cutaneous wound healing is a major complication in patients with diabetes mellitus (DM), leading to increased amputation and mortality rates affected patients. Adipose-derived stem cells (ASCs) are widely used seed for promoted tissue regeneration improve closure under diabetic conditions. However, ASCs-based therapies remain limited due difficulties maintaining cell quality during transplantation. To overcome this problem, extracellular matrix mimetic biomaterials have been...
Common 2-dimensional (2D) cell cultures do not adequately represent cell-cell and cell-matrix signaling substantially different diffusion/transport pathways. To obtain tissue-mimic information on nanoparticle toxicity from in vitro tests, we used a 3-dimensional (3D) culture of human lung cells (A549) prepared with elastin-like peptides modified an arginine-glycine-aspartate motif. The 3D showed cellular phenotypes, gene expression profiles, functionalities compared to the 2D cultured cells....
Osteoclasts are the only cell type capable of resorbing mineralized bone, and they act under control numerous cytokines produced by supporting cells such as osteoblasts stromal cells. Among cytokines, receptor activator nuclear factor-κB ligand (RANKL) was found to be a key osteoclastogenetic molecule that directly binds its cognate receptor, RANK, on osteoclast precursor In turn, RANKL, which is an essential factor for differentiation activation osteoclasts, one major targets...
In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells spheroids. For purpose, established spheroid formation by growing SH-SY5Y on hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days culture, relative proliferation within spheroids was approximately 92% values for cultures. As measured quantitative assays mRNA protein expressions, production synaptophysin neuronspecific enolase (NSE) as well contents cell...
With the remarkable increase in fields of biomedical engineering and regenerative medicine, biomaterial design has become an indispensable approach for developing biocompatible carriers drug or gene cargo extracellular matrix (ECM) cell survival, proliferation differentiation. Native ECM materials derived from animal tissues were believed to be best choices tissue engineering. However, possible pathogen contamination by cellular remnants foreign is unavoidable issue that limited use native...