A5100743444- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Ginseng Biological Effects and Applications
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Cardiomyopathy and Myosin Studies
- Insect and Pesticide Research
- Bee Products Chemical Analysis
- Healthcare and Venom Research
- Animal Genetics and Reproduction
- Crystallization and Solubility Studies
- 3D Printing in Biomedical Research
- X-ray Diffraction in Crystallography
- Blood Pressure and Hypertension Studies
- Cellular Mechanics and Interactions
- Cardiovascular Function and Risk Factors
- Neuroscience and Neuropharmacology Research
- biodegradable polymer synthesis and properties
- Liver physiology and pathology
- Atrial Fibrillation Management and Outcomes
- Pharmacological Effects of Natural Compounds
- Cardiovascular Effects of Exercise
- Connective tissue disorders research
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Disease and Adiposity
Yale University
2014-2024
Hanyang University Medical Center
2012-2024
Hallym University
2024
Kyung Hee University
2018-2024
Hallym Polytechnic University
2024
Yale Cancer Center
2019-2024
Kyungpook National University
2016-2023
Severance Hospital
2023
Yonsei University
2014-2023
Cheju Halla General Hospital
2023
Melittin, the main peptide present in bee venom, has been proposed as having potential for anticancer therapy; addition of melittin to cisplatin, a first line treatment ovarian cancer, may increase therapeutic response cancer via synergy, resulting improved tolerability, reduced relapse, and decreased drug resistance. Thus, this study was designed compare metabolomic effects combination with cisplatin cisplatin-sensitive (A2780) resistant (A2780CR) cells. Liquid chromatography (LC) coupled...
Hypertrophic cardiomyopathy (HCM) is often caused by single sarcomeric gene mutations that affect muscle contraction. Pharmacological correction of mutation effects prevents but does not reverse disease in mouse models. Suspecting diseased extracellular matrix to blame, we obtained myocardium from a miniature swine model HCM, decellularized thin slices the tissue, and re-seeded them with healthy human induced pluripotent stem cell–derived cardiomyocytes. Compared cardiomyocytes grown on...
Background: Familial hypertrophic cardiomyopathy (HCM) is the most common inherited cardiac disease and typically caused by mutations in genes encoding sarcomeric proteins that regulate contractility. HCM manifestations include left ventricular hypertrophy heart failure, arrythmias, sudden death. How dysregulated force production sensed leads to pathological remodeling remains poorly understood HCM, thereby inhibiting efficient development of new therapeutics. Methods: Our discovery was...
Since pluripotent embryonic stem cell (ESC) lines were first derived from the mouse, tremendous efforts have been made to establish ESC in several domestic species including pig; however, authentic porcine ESCs not yet established. It has proven difficult maintain an ESC-like state due frequent occurrence of spontaneous differentiation into epiblast (EpiSC)-like during culture. We able derive EpiSC-like (pESC) blastocyst stage embryos various origins, vitro fertilized (IVF), vivo derived,...
Current long term cryopreservation of cell stocks routinely requires the use liquid nitrogen (LN2), because commonly used media containing membrane permeating cryoprotectants are thermally unstable when frozen at higher storage temperatures, e.g. -80 °C. This instability leads to ice recrystallization, causing progressive loss viability over time under conditions provided by most laboratory deep freezers. The dependency on LN2 for significantly increases operational expense and raises issues...
Abstract The transmembrane death receptor Fas transduces apoptotic signals upon binding its ligand, FasL. Although is highly expressed in cancer cells, insufficient cell surface expression desensitizes cells to Fas-induced apoptosis. Here, we show that the increase microaggregate formation on plasma membrane response inhibition of endocytosis sensitizes We used a clinically accessible Rho-kinase inhibitor, fasudil, reduces dynamics by increasing tension. In combination with exogenous soluble...
Arrhythmogenic cardiomyopathy (ACM) is an inherited disorder with variable genetic etiologies. Here, we focused on understanding the precise molecular pathology of a single clinical variant in DSP, gene encoding desmoplakin. We initially identified potentially novel missense desmoplakin (p.R451G) patient diagnosed biventricular ACM. An extensive single-family ACM cohort was assembled, revealing pattern coinheritance for R451G and phenotype. vitro model system using patient-derived induced...
Hypertrophic cardiomyopathy (HCM) is an inherited disorder caused primarily by mutations to thick and thinfilament proteins. Although thin filament are less prevalent than their oft-studied counterparts, they frequently associated with severe patient phenotypes can offer important insight into fundamental disease mechanisms. We have performed a detailed study of tropomyosin (TPM1) E192K, variant uncertain significance HCM. Molecular dynamics revealed that E192K results in more flexible TPM1...
Abstract The CRISPR/Cas9 system easily edits target genes in various organisms and is used to treat human diseases. In most therapeutic CRISPR studies, ubiquitously expressed promoters, such as CMV, CAG, EF1α, are used; however, gene editing sometimes necessary only specific cell types relevant the disease. Therefore, we aimed develop a retinal pigment epithelium (RPE)-specific system. We developed that operates (RPE) by expressing Cas9 under RPE-specific vitelliform macular dystrophy 2...
Epilepsy, a complex neurological disorder, is characterized by recurrent seizures caused aberrant electrical activity in the brain. Central to this study role of lysosomal dysfunction epilepsy, which can lead accumulation toxic substrates and impaired autophagy neurons. Our focus on phosphodiesterase-4 (PDE4), an enzyme that plays crucial regulating intracellular cyclic adenosine monophosphate (cAMP) levels converting it into (AMP). In pathological states, including increased PDE4...
Naked mole rats (NMRs) are exceptionally long-lived, cancer-resistant rodents. Identifying the defining characteristics of these traits may shed light on aging and cancer mechanisms. Here, we report generation induced pluripotent stem cells (iPSCs) from NMR fibroblasts their contribution to mouse-NMR chimeric embryos. Efficient reprogramming could be observed under N2B27+2i conditions. The iPSCs displayed a characteristic morphology, expressed markers, formed embryoid bodies, showed typical...
Cellular reprogramming of committed cells into a pluripotent state can be induced by ectopic expression genes such as OCT4, SOX2, KLF4, and MYC. Reprogrammed maintained activating endogenous networks without transgene expression. Although various research groups have attempted to generate pig stem (iPSCs), authentic iPSCs not obtained, instead showing dependence on In this study, were derived from porcine fetal fibroblasts via drug-inducible vectors carrying human transcription factors...
Background and Objectives: Electrocardiography (ECG) is a cost-effective useful method for diagnosing left ventricular hypertrophy (LVH) in large-scale study or clinical practice.Among ECG criteria, the Cornell product (Cor P) Sokolow-Lyon criteria were adopted by European Society of Hypertension-European Cardiology Guidelines but have different performances among races.The aim this was to compare diagnostic performance two voltage Korean patients.Subjects Methods: echocardiographic LV mass...
The prevalence of cardiovascular disease in women increases sharply after menopause. purpose this study was to clarify the relationship between menopause and body fat distribution investigate their association with risk factors.We analyzed 2035 20-79 years age using National Health Nutrition Examination Survey (KNHANES) 2010 database. Body measured dual-energy X-ray absorptiometry.The percentage total (BFD) index (the ratio trunk mass leg mass) are significantly higher postmenopausal than...