A5045308226- Retinal Development and Disorders
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Force Microscopy Techniques and Applications
- Nerve injury and regeneration
- Cellular Mechanics and Interactions
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- 3D Printing in Biomedical Research
- Child Abuse and Related Trauma
- Hippo pathway signaling and YAP/TAZ
- Glaucoma and retinal disorders
- Axon Guidance and Neuronal Signaling
- Cardiovascular Effects of Exercise
- Neurogenesis and neuroplasticity mechanisms
- Cardiomyopathy and Myosin Studies
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Sports injuries and prevention
- Traumatic Ocular and Foreign Body Injuries
University of California, San Francisco
2018-2024
Yale University
2019
Arrhythmogenic cardiomyopathy (ACM) is an inherited disorder with variable genetic etiologies. Here, we focused on understanding the precise molecular pathology of a single clinical variant in DSP, gene encoding desmoplakin. We initially identified potentially novel missense desmoplakin (p.R451G) patient diagnosed biventricular ACM. An extensive single-family ACM cohort was assembled, revealing pattern coinheritance for R451G and phenotype. vitro model system using patient-derived induced...
Serotonin (5-HT) is known to increase the rate of growth cone advance via cofilin-dependent increases in retrograde actin network flow and nonmuscle myosin II activity. We report that activity regulated by PKC during 5-HT responses necessary for traction force normally associated with these responses. simultaneously induces decreases density PKC-dependent point contact density. These reciprocal effects facilitate production domains exhibiting decreased Interestingly, when was up-regulated,...
Maintaining precise synaptic contacts between neuronal partners is critical to ensure the proper functioning of mammalian central nervous system (CNS). Diverse cell recognition molecules, such as classic cadherins (Cdhs), are part molecular machinery mediating choices during development and maintenance. Yet, principles governing neuron–neuron wiring across diverse CNS neuron types remain largely unknown. The retinotectal synapses, connections from retinal ganglion cells (RGCs) superior...
Abstract Purpose of Review Traumatic optic neuropathy (TON) is a devasting disorder that can result in irreversible vision loss. Understanding the current research to promote neuroprotection and neuroregeneration nerve after injury may shed light on promising therapeutic avenues. Recent Findings With evolving methods model traumatic neuropathy, recent work manipulating signal transduction cell damage response pathways reveals new clinical opportunities for patients with nerve. Summary...
Diverse retinal ganglion cells (RGCs) transmit distinct visual features from the eye to brain. Recent studies have categorized RGCs into 45 types in mice based on transcriptomic profiles, showing strong alignment with morphological and electrophysiological properties. However, little is known about how these are spatially arranged two-dimensional surface-an organization that influences encoding-and their local microenvironments impact development neurodegenerative responses. To address this...
Abstract Vision starts with image formation at the retina, which contains diverse neuronal cell types that extract, process, and relay visual information to higher order processing centers in brain. Though there has been steady progress defining retinal types, very little is known about development humans, well before birth. In this study, we performed transcriptomic profiling of developing human fetal retina from gestational weeks 12 27 using single-cell RNA-seq (scRNA-seq) used pseudotime...
Summary The development of single-cell RNA-Sequencing (scRNA-Seq) has allowed high resolution analysis cell type diversity and transcriptional networks controlling fate specification. To identify the governing human retinal development, we performed scRNA-Seq over organoid in vivo across 20 timepoints. Using both pseudotemporal cross-species analyses, examined conservation gene expression progenitor maturation specification all seven major types. Furthermore, differences between developing...
The development of single-cell RNA-Sequencing (scRNA-Seq) has allowed high resolution analysis cell type diversity and transcriptional networks controlling fate specification. To identify the governing human retinal development, we performed scRNA-Seq over organoid in vivo across 20 timepoints. Using both pseudotemporal cross-species analyses, examined conservation gene expression progenitor maturation specification all seven major types. Furthermore, differences between developing macula...