A5051034595- Congenital gastrointestinal and neural anomalies
- Intestinal Malrotation and Obstruction Disorders
- Congenital Anomalies and Fetal Surgery
- Gastrointestinal motility and disorders
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
- Glaucoma and retinal disorders
- Infant Nutrition and Health
- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Congenital Diaphragmatic Hernia Studies
- Circadian rhythm and melatonin
- Erythrocyte Function and Pathophysiology
- Digestive system and related health
- Congenital heart defects research
- Genetics, Aging, and Longevity in Model Organisms
- Hedgehog Signaling Pathway Studies
- Hippo pathway signaling and YAP/TAZ
- Infant Health and Development
- Clostridium difficile and Clostridium perfringens research
- Pediatric Hepatobiliary Diseases and Treatments
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
University of California, San Francisco
2020-2025
Harvard University
2015-2021
Massachusetts General Hospital
2015-2021
Brown University
2018
Mechanisms mediating adult enteric neurogenesis are largely unknown. Using inflammation-associated models and a transgenic approach, we aimed to understand the cell-source for new neurons in infectious inflammatory colitis. Dextran sodium sulfate (DSS) Citrobacter rodentium colitis (CC) was induced mice colonic were quantified. Sox2GFP PLP1GFP confirmed cell-type specificity of these markers. Sox2CreER:YFP PLP1creER:tdT used determine fate cells after Sox2 expression investigated human...
The intestine is known to contain enteric neuronal progenitors, but their precise identity and the mechanisms that activate them remain unknown. Based on evidence for neurogenic role of serotonin (5-HT) in postnatal gut observation hyperplasia inflammatory bowel disease, we hypothesized colitis induces a response through 5-HT4 receptor signaling.We examined effects agonism colonic neurogenesis gliogenesis vitro vivo adult mice using dextran sodium sulfate experimentally induce colitis.In...
Abstract Background Transplanting autologous patient‐derived enteric neuronal stem/progenitor cells ( ENSC s) is an innovative approach to replacing missing neurons in patients with Hirschsprung disease HSCR ). Using eliminates immunologic and ethical concerns raised by other cell sources. However, whether postnatal aganglionic bowel permissive for transplanted s from can be successfully isolated, cultured, vivo remains unknown. Methods isolated the ganglionic intestine of Ednrb −/− mice ‐...
The enteric nervous system (ENS) develops from neural crest cells that migrate along the intestine, differentiate into neurons and glia, pattern two plexuses within gut wall. Inductive interactions between epithelium mesenchyme regulate development, but influence of these on ENS development is unknown. Epithelial-mesenchymal recombinations were constructed using avian hindgut non-intestinal bursa Fabricius. These led to abnormally large ectopically positioned ganglia. We hypothesized Sonic...
Neurons use cell-adhesion molecules (CAMs) to interact with other neurons and the extracellular environment: combination of CAMs specifies migration patterns, neuronal morphologies, synaptic connections across diverse neuron types. Yet little is known regarding intracellular signaling cascade mediating CAM recognitions at cell surface different In this study, we investigated neural developmental role Afadin 1–4 , a cytosolic adapter protein that connects multiple families F-actin. We...
Neurons use cell-adhesion molecules (CAMs) to interact with other neurons and the extracellular environment: combination of CAMs specifies migration patterns, neuronal morphologies, synaptic connections across diverse neuron types. Yet little is known regarding intracellular signaling cascade mediating CAM recognitions at cell surface different In this study, we investigated neural developmental role Afadin 1–4 , a cytosolic adapter protein that connects multiple families F-actin. We...
Abstract Background Transplantation of enteric neural stem cells ( ENSC ) holds promise as a potential therapy for neuropathies, including Hirschsprung disease. Delivery transplantable via laparotomy has been described, but we propose novel, minimally invasive endoscopic method cell delivery. Methods Enteric transplantation were cultured from dissociated gut postnatal donor mice. Twelve recipient mice, Ednrb −/− mice with distal colonic aganglionosis, underwent colonoscopic injection under...
Abstract The development of the enteric nervous system ( ENS ) and intestinal smooth muscle occurs in a spatially temporally correlated manner, but how they influence each other is unknown. In developing mid‐gut chick embryo, we find that α‐smooth actin expression, indicating early differentiation, after arrival migrating neural crest‐derived cells ENCC s). contrast, hindgut develops prior to arrival. Smooth normal experimentally aganglionic hindguts, suggesting proper patterning layers does...
The enteric nervous system (ENS) develops from neural crest cells that migrate along the intestine, differentiate into neurons and glia, pattern two plexuses within gut wall.Inductive interactions between epithelium mesenchyme regulate development, but influence of these on ENS development is unknown.Epithelial-mesenchymal recombinations were constructed using avian hindgut nonintestinal bursa Fabricius.These led to abnormally large ectopically positioned ganglia.We hypothesized sonic...
ABSTRACT The enteric nervous system (ENS), which is derived from neural crest cells (ENCCs), represents the neuronal innervation of intestine. Compromised ENCC migration can lead to Hirschsprung disease, characterized by an aganglionic distal bowel. During craniocaudal ENCCs along gut, we find that their proliferation greatest as wavefront passes through ceca, a pair pouches at midgut-hindgut junction in avian Removal ceca leads hindgut aganglionosis, suggesting they are required for ENS...
The role of Notch signaling in cell-fate decisions has been studied extensively; however, this pathway is also active adult tissues, including the nervous system. modulates a wide range behaviors and processes system nematode Caenorhabditis elegans, but there no evidence for directly altering synaptic strength. Here, we demonstrate Notch-mediated regulation activity at C. elegans neuromuscular junction (NMJ). For this, used aldicarb, an inhibitor enzyme acetylcholinesterase, assessed...
ABSTRACT Neurons use cell-adhesion molecules (CAMs) to interact with other neurons and the extracellular environment: combination of CAMs specifies migration patterns, neuronal morphologies, synaptic connections across diverse neuron types. Yet little is known regarding intracellular signaling cascade mediating CAM recognitions at cell surface different In this study, we investigated neural developmental role Afadin 1–4 , a cytosolic adapter protein that connects multiple families F-actin....