A5100348104- Hippo pathway signaling and YAP/TAZ
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Cancer, Lipids, and Metabolism
- Colorectal Cancer Treatments and Studies
- Hepatitis B Virus Studies
- Melanoma and MAPK Pathways
- ATP Synthase and ATPases Research
- NF-κB Signaling Pathways
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Lipoproteins and Cardiovascular Health
- Lipid metabolism and biosynthesis
- Multiple and Secondary Primary Cancers
- Biosensors and Analytical Detection
- Receptor Mechanisms and Signaling
- Proteoglycans and glycosaminoglycans research
First Affiliated Hospital of Xi'an Jiaotong University
2024
Sir Run Run Shaw Hospital
2024
Zhejiang University
2024
Shandong First Medical University
2024
Capital Medical University
2023
First Affiliated Hospital of Nanchang University
2023
Nanchang University
2023
Chinese Academy of Sciences
2011-2022
Institute of Microbiology
2011-2022
Jiangnan University
2020
Hepatitis B virus (HBV) causes chronic infection in about 350 million people worldwide. Given the important role of most abundant liver-specific microRNA, miR-122, hepatic function and liver pathology, here we investigated potential mechanism miR-122 regulating HBV replication. We found that expression was significantly down-regulated patients with compared healthy controls, levels were negatively correlated intrahepatic viral load necroinflammation. The depletion endogenous by its antisense...
Abstract The Hippo pathway is a key growth control that conserved across species. downstream effectors of the pathway, YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif), are frequently activated in cancers to drive proliferation survival. Based on premise sustained interactions between YAP/TAZ TEADs enhanced associate domain) central their transcriptional activities, we discovered potent small-molecule inhibitor (SMI), GNE-7883, allosterically blocks...
The common neurotrophin receptor, p75NTR, has been shown to signal in the absence of Trk tyrosine kinase receptors, including induction neural apoptosis and activation NF-κB. However, mechanisms by which p75NTR initiates these intracellular transduction pathways are unknown. Here we report interactions between six members TRAF (tumor necrosis factor receptor-associated factors) family proteins. binding different proteins was mapped distinct regions p75NTR. Furthermore, TRAF4 interacted with...
// Xue Yang 1, * , Minglei Zhuo Xin Ye 2 Hua Bai 1 Zhijie Wang Yun Sun Jun Zhao Tongtong An Jianchun Duan Meina Wu Jie Department of Thoracic Medical Oncology, Key Laboratory Carcinogenesis and Translational Research (Ministry Education), Peking University Cancer Hospital Institute, Beijing, China Asia Emerging Markets Innovative Medicine AstraZeneca R & D, Shanghai, These authors contributed equally to this work Correspondence to: Wang, e-mail: zlhuxi@163.com Keywords: non-small-cell lung...
Manganese (Mn) accumulates in the central nervous system and can cause neurotoxicity, but mechanisms of Mn-induced neurotoxicity remain unclear. We performed single-cell RNA sequencing (scRNA-seq) zebrafish brain after Mn exposure identified 10 cell types by marker genes: cholinergic neurons, dopaminergic (DA) glutaminergic GABAergic neuronal precursors, other microglia, oligodendrocyte, radial glia, undefined cells. Each type has its distinct transcriptome profile. Pseudotime analysis...
Formaldehyde (FA), a major industrial chemical and ubiquitous environmental pollutant, has been classified as leukemogen. The causal relationship remains unclear, however, due to limited evidence that FA induces toxicity in bone marrow, the site of leukemia induction, other distal organs. Although induction DNA–protein crosslinks (DPC), hallmark toxicity, was not previously detected marrow FA‐exposed rats monkeys studies published 1980s, our recent showed increased DPC liver, kidney, testes...
Abstract Thyroid cancer (TC) is the most frequent malignancy of endocrine system. Apatinib, as an anti-angiogenic agent, has been applied in therapy several cancers. However, function and mechanism Apatinib TC have not clearly elucidated. After processing with alone or combined PKM2 overexpression plasmids, cell proliferation, migration, invasion were analyzed by EdU staining, CCK-8, wound healing, Transwell. Meanwhile. HUVECs incubated conditioned medium prepared from culture medium, tube...
World model based reinforcement learning (RL) has emerged as a promising approach for autonomous driving, which learns latent dynamics and uses it to train planning policy. To speed up the process, pretrain-finetune paradigm is often used, where online RL initialized by pretrained policy learned offline. However, naively performing such initialization in may result dramatic performance degradation during interactions new task. tackle this challenge, we first analyze identify two primary root...
Background: Divarasib, a covalent inhibitor targeting the Kirsten rat sarcoma virus oncogene homologue glycine-to-cysteine mutation at position 12 (KRAS G12C), is currently in clinical development for Non Small Cell Lung Cancer (NSCLC) treatment, with various combination partners, such as Src homology region 2 domain-containing phosphatase-2 (SHP2) migoprotafib. A quantitative systems pharmacology (QSP) model essential to quantitatively assess single-agent and pharmacodynamic (PD) effects...
Ferroptosis is an iron-dependent cell death mechanism characterized by the accumulation of toxic lipid peroxides and membrane rupture. GPX4 (glutathione peroxidase 4) prevents ferroptosis reducing these into alcohols. induction inhibition has emerged as a vulnerability cancer cells, highlighting need to identify regulators that may be exploited therapeutically. Through genome-wide CRISPR activation screens, we SWI/SNF (switch/sucrose non-fermentable) ATPases BRM (SMARCA2) BRG1 (SMARCA4)...
Circulating tumor cells (CTCs) acting as "liquid biopsy" of cancer are that have been shed from the primary tumor, which cause development a secondary in distant organ site, leading to metastasis. Recent research suggests CTCs with abnormalities gene copy numbers mononuclear cell-enriched peripheral blood samples, namely circulating genetically abnormal (CACs), could be used non-invasive decision tool detect patients benign pulmonary nodules. Such identified by counting fluorescence signals...
Modulation of proteolysis is an emerging therapeutic mainstay. The clinical success thalidomide and analogs has inspired development rationally-designed therapeutics that repurpose endogenous degradation machinery to target pathogenic proteins. However, it unknown whether removal the critical effect drives degrader-induced efficacy. Here we report proteasome-generated peptides actively initiate cell death. Utilizing BET family degraders as exemplars, find induced proteasomal BRD4-long...
Genomic profiling using plasma cell-free DNA (cfDNA) represents a non-invasive alternative to tumor re-biopsy, which is challenging in clinical practice. The feasibility of dynamically monitoring epidermal growth factor receptor (EGFR) mutation status serial samples from non-small cell lung cancer (NSCLC) patients treated by tyrosine kinase inhibitors (TKIs) and its application tracking response detection resistance were investigated.Forty-five NSCLC with EGFR mutation-positive pre-TKI at...
Background Circulating genetically abnormal cells (CACs) with specific chromosome variations have been confirmed to be present in non‐small cell lung cancer (NSCLC). However, the diagnostic performance of CAC detection remains unclear. This study aimed evaluate potential clinical application test for early diagnosis NSCLC. Methods In this prospective study, a total 339 participants (261 patients and 78 healthy volunteers) were enrolled. An antigen‐independent fluorescence situ hybridization...
There is a growing interest in screening α-amylase inhibitors from natural products for application the development of new antidiabetic drugs or functional foods. In this study, structure-based virtual was applied to rapidly identify medicine food homology (MFH) plants. Similarity search, docking & scoring were used further filter small molecules. As result, 21 corresponding potential MFH plants obtained. And, six polyphenol compounds (curcumin, procyanidins, epicatechin gallate (ECG),...
The transcription factor NF-κB p65 is a key regulator in the regulation of an inflammatory response and pathology atherosclerosis. However, there no direct evidence for role macrophages development We investigated whether macrophage overexpression apoE-knockout mice could improve Transgenic (Tg) overexpressing were generated by crossing fatty acid-binding protein 4 (aP2) promoter-controlled with (KO) mice. Tg exhibited functional activation signaling fat tissues. observed that...
The Hippo pathway is among the most frequently altered key signaling pathways in cancer. TEAD1-4 are essential transcription factors and downstream effectors pathway. Here we identified RNF146 as a ubiquitin ligase (E3) that can catalyze TEAD ubiquitination negatively regulate their function cells. We show this of TEADs governed by PARylation state validated genetic interaction between cancer cell lines model organism Drosophila melanogaster. Furthermore, demonstrate pharmacologically...
Osteoarthritis (OA) is a degenerative joint disease characterized by the progressive degeneration of articular cartilage, leading to pain, stiffness, and loss function. The pathogenesis OA involves multiple factors, including increased intracellular reactive oxygen species (ROS), enhanced chondrocyte apoptosis, disturbances in cartilage matrix metabolism. These processes contribute breakdown extracellular (ECM) integrity, ultimately resulting damage dysfunction. RNA interference (RNAi)...
The Hippo pathway is among the most frequently altered key signaling pathways in cancer. TEAD1-4 are essential transcription factors and downstream effectors human cells. Here, we identified RNF146 as a ubiquitin ligase (E3) of TEADs, which negatively regulates their stability cells through proteasome-mediated degradation. We show that RNF146-mediated TEAD ubiquitination dependent on PARylation state. further validated genetic interaction between cancer cell lines model organism Drosophila...