A5041738065- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Fungal Biology and Applications
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Viral gastroenteritis research and epidemiology
- Biocrusts and Microbial Ecology
- Enzyme Production and Characterization
- Cell Image Analysis Techniques
- Advanced Chemical Sensor Technologies
- RNA and protein synthesis mechanisms
- Enzyme-mediated dye degradation
- Genetics, Aging, and Longevity in Model Organisms
- Fermentation and Sensory Analysis
- Enzyme function and inhibition
- Food Quality and Safety Studies
- Enzyme Catalysis and Immobilization
- Plant Pathogens and Fungal Diseases
- Cancer-related molecular mechanisms research
- Heat shock proteins research
- Transgenic Plants and Applications
- Fungal and yeast genetics research
Fujian Agriculture and Forestry University
2018-2025
Chinese Academy of Sciences
2010-2016
Institute of Zoology
2016
Institute of Microbiology
2010-2012
Hepatitis B virus (HBV) causes chronic infection in about 350 million people worldwide. Given the important role of most abundant liver-specific microRNA, miR-122, hepatic function and liver pathology, here we investigated potential mechanism miR-122 regulating HBV replication. We found that expression was significantly down-regulated patients with compared healthy controls, levels were negatively correlated intrahepatic viral load necroinflammation. The depletion endogenous by its antisense...
ABSTRACT Alpha interferon (IFN-α)-based therapy can effectively treat chronic hepatitis B virus (HBV) infection, which causes life-threatening complications. Responses to IFN-α vary greatly in (CHB) patients, but underlying mechanisms are almost unknown. In this study, we found that treatment induced a marked decrease of microRNA-122 (miR-122) expression hepatocytes. We next showed IFN-α-induced miR-122 downregulation was only partly due transcriptional suppression. One IFN-stimulated gene...
Abstract The POU domain transcription factor Oct4 plays critical roles in self-renewal and pluripotency of embryonic stem cells (ESCs). Together with Sox2, Klf4 c-Myc, can reprogram any other cell types to pluripotency, which is the only that cannot be functionally replaced by family members. To investigate determinant elements uniqueness, we performed Ala scan on all Ser, Thr, Tyr, Lys Arg murine testing their capability somatic reprogramming. We uncovered a series residues are important...
(3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed develop a whole-cell biocatalysis strategy for (3S)-acetoin from meso-2,3-butanediol. First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase Vitreoscilla hemoglobin was developed Maximum concentration 72.38 g/L with stereoisomeric...
Somatic cell reprogramming toward induced pluripotent stem cells (iPSCs) holds great promise in future regenerative medicine. However, the process mediated by traditional defined factors (OSMK) is slow and extremely inefficient. Here, we develop a combination of modified (OySyNyK) which transactivation domain Yes-associated protein fused to establish highly efficient rapid system. We show that efficiency OySyNyK-induced iPSCs up 100-fold higher than OSNK OySyNyK very initiated 24 hr. find...
In this study, a novel laccase gene (Lcc1) from Ganoderma tsugae was isolated and its functions were characterized in detail. The results showed that Lcc1 has the highest expression activity during mycelium development fruit body maturation based on analysis of RNA transcripts at different developmental stages G. tsugae. To investigate exact contribution to tsugae, transgenic strains constructed by targeted replacement over-expression approaches. lignin degradation rate deletion mutant much...
In this study, fresh Lyophyllum decastes was dried using hot air drying (HAD), combined with vacuum (HAVD), and freeze (VFD). Additionally, the quality volatile compounds were analyzed. VFD achieved best color retention, highest rehydration capacity, slightest damaged tissue structure; however, it recorded longest time energy consumption. HAD most energy-efficient of three methods. Furthermore, products more hardness elasticity obtained by HAVD—this finding convenient for transportation....