A5046149222- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Metabolism and Genetic Disorders
- Marine and coastal ecosystems
- Gene expression and cancer classification
- Light effects on plants
- Molecular Biology Techniques and Applications
- Ion Transport and Channel Regulation
- Pancreatic function and diabetes
- Aquatic Ecosystems and Phytoplankton Dynamics
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Renal and related cancers
CEA Cadarache
2011-2022
Institut de Biosciences et Biotechnologies
2016-2022
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
1999-2022
Centre National de la Recherche Scientifique
1999-2017
Aix-Marseille Université
2008-2017
Institute of Environmental Biology and Biotechnology
2011
Algal enzyme driven by blue light Microalgae make hydrocarbons. In searching for the responsible, Sorigué et al. found a glucose-methanolcholine oxidoreductase (see Perspective Scrutton). Expression of in Escherichia coli showed that hydrocarbon production requires visible light. fact, constant input photons to carry out its catalytic reaction. A long hydrophobic tunnel stabilizes fatty acid substrates proximity flavin adenine dinucleotide cofactor. Science , this issue p. 903 ; see also 872
Abstract Oil bodies are sites of energy and carbon storage in many organisms including microalgae. As a step toward deciphering oil accumulation mechanisms algae, we used proteomics to analyze purified from the model microalga Chlamydomonas reinhardtii grown under nitrogen deprivation. Among 248 proteins (≥2 peptides) identified by LC‐MS/MS, 33 were putatively involved metabolism lipids (mostly acyl‐lipids sterols). Compared with recently reported body proteome, 19 new lipid identified,...
Electron transfer pathways associated to oxygenic photosynthesis, including cyclic electron flow around photosystem I and chlororespiration, rely on non-photochemical reduction of plastoquinones (PQs). In higher plant chloroplasts, a bacterial-like NDH complex homologous is involved in PQ reduction, but such absent from Chlamydomonas plastids where type II NAD(P)H dehydrogenase activity has been proposed operate. With the aim elucidate nature enzyme-supporting PQs, one dehydrogenases...
Large-scale gene expression studies can now be routinely performed on macroamounts of cells, but it is unclear to which extent current methods are valuable for analyzing complex tissues. In the present study, we used method serial analysis (SAGE) quantitative mRNA profiling in mouse kidney. We first SAGE at whole-kidney level by sequencing 12,000 tags. Most abundant tags corresponded transcripts widely distributed or enriched predominant kidney epithelial cells (proximal tubular cells),...
Because of their high biomass productivity and ability to accumulate levels energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production biofuel. Accumulation takes place when face adverse situations nutrient shortage, conditions which also provoke stop in cell division, down-regulation photosynthesis. Despite growing interest microalgal biofuels, little is known about molecular mechanisms controlling carbon formation. In order discover...
Nitrogen (N) starvation-induced triacylglycerol (TAG) synthesis, and its complex relationship with starch metabolism in algal cells, has been intensively studied; however, few studies have examined the interaction between amino acid TAG biosynthesis. Here, via a forward genetic screen for homeostasis, we isolated Chlamydomonas (Chlamydomonas reinhardtii) mutant (bkdE1α) that is deficient E1α subunit of branched-chain ketoacid dehydrogenase (BCKDH) complex. Metabolomics analysis revealed...
Abstract Microalgae have emerged as a promising source for biofuel production. Massive oil and starch accumulation in microalgae is possible, but occurs mostly when biomass growth impaired. The molecular networks underlying the negative correlation between reserve formation are not known. Thus isolation of strains capable accumulating carbon reserves during optimal would be highly desirable. To this end, we screened an insertional mutant library Chlamydomonas reinhardtii alterations content....
ABSTRACT Photosynthetic organisms have developed sophisticated strategies to fine-tune light energy conversion meet the metabolic demand, thereby optimizing growth in fluctuating environments. Although mechanisms such as dissipation, photosynthetic control, or photosystem II (PSII) damage and repair been widely studied, little is known about regulation of protein synthesis capacity during acclimation. By screening a Chlamydomonas reinhardtii insertional mutant library using chlorophyll...