A5085781001- Lipid metabolism and biosynthesis
- Oil Palm Production and Sustainability
- Coconut Research and Applications
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Algal biology and biofuel production
- Plant Molecular Biology Research
- Coastal wetland ecosystem dynamics
- Plant responses to water stress
- Marine and coastal plant biology
- Constructed Wetlands for Wastewater Treatment
- Food Chemistry and Fat Analysis
- Peroxisome Proliferator-Activated Receptors
- GABA and Rice Research
- Enzyme Catalysis and Immobilization
- Cassava research and cyanide
- Proteins in Food Systems
- Pancreatic function and diabetes
- Research in Cotton Cultivation
Brookhaven National Laboratory
2022-2024
Institut de Biosciences et Biotechnologies
2017-2019
Aix-Marseille Université
2017-2018
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2018
Centre National de la Recherche Scientifique
2017-2018
Hainan University
2012-2018
Summary Peroxisomes are thought to have played a key role in the evolution of metabolic networks photosynthetic organisms by connecting oxidative and biosynthetic routes operating different compartments. While various pathways peroxisomes higher plants fairly well characterized, reactions present primitive (microbodies) algae poorly understood. Screening Chlamydomonas insertional mutant library identified strain strongly impaired oil remobilization defective Cre05.g232002 ( CrACX2 ), gene...
Enriching algal biomass in energy density is an important goal biotechnology. Nitrogen (N) starvation considered the most potent trigger of oil accumulation microalgae and has been thoroughly investigated. However, N causes slow down eventually arrest growth. In this study, we show that exposing a Chlamydomonas reinhardtii culture to saturating light (SL) under nonlimiting CO2 concentration turbidostatic photobioreactors induces sustained lipid droplets (LDs) without compromising growth,...
Plants and algae must tightly coordinate photosynthetic electron transport metabolic activities given that they often face fluctuating light nutrient conditions. The exchange of metabolites signaling molecules between organelles is thought to be central this regulation but evidence for still fragmentary. Here, we show knocking out the peroxisome-located MALATE DEHYDROGENASE2 (MDH2) Chlamydomonas reinhardtii results in dramatic alterations not only peroxisomal fatty acid breakdown also...
Summary Duckweeds are amongst the fastest growing of higher plants, making them attractive high‐biomass targets for biofuel feedstock production. Their fronds have high rates fatty acid synthesis to meet demand new membranes, but triacylglycerols (TAG) only accumulate very low levels. Here we report on engineering Lemna japonica and accumulation TAG in its fronds. This was achieved by expression an estradiol‐inducible cyan fluorescent protein‐ Arabidopsis WRINKLED1 fusion protein (...
Summary Over 15 families of aquatic plants are known to use a strategy developmental switching upon environmental stress produce dormant propagules called turions. However, few molecular details for turion biology have been elucidated due the difficulties in isolating high‐quality nucleic acids from this tissue. We successfully developed new protocol isolate transcripts and carried out RNA‐seq analysis mature turions Greater Duckweed Spirodela polyrhiza . Comparison transcriptomes that...
Summary Triacylglycerols (TAG), accumulate within lipid droplets (LD), predominantly surrounded by OLEOSINs (OLE), that protect TAG from hydrolysis. We tested the hypothesis identifying and removing degradation signals OLE would promote its abundance, preventing enhancing accumulation. whether mutating potential ubiquitin‐conjugation sites in a previously reported improved Sesamum indicum ( SiO ) variant, o3‐3 Cys‐OLE SiCO herein), stabilize it increase lipogenic potential. SiCOv1 was...
Camelina (Camelina sativa L.), a hexaploid member of the Brassicaceae family, is an emerging oilseed crop being developed to meet increasing demand for plant oils as biofuel feedstocks. In other Brassicas, high oil content can be associated with yellow seed phenotype, which unknown camelina. We sought create camelina using CRISPR/Cas9 technology disrupt its Transparent Testa 8 (TT8) transcription factor genes and evaluate resulting phenotype. identified three TT8 genes, one in each...
Metabolic engineering for hyperaccumulation of lipids in vegetative tissues is a novel strategy enhancing energy density and biofuel production from biomass crops. Energycane prime feedstock this approach due to its high resilience under marginal conditions. DIACYLGLYCEROL ACYLTRANSFERASE (DGAT) catalyzes the last only committed step biosynthesis triacylglycerol (TAG) can be rate-limiting enzyme TAG.In study, we explored effect intron-mediated enhancement (IME) on expression DGAT1 resulting...
Oil palm (Elaeis guineensis Jacq.) is the highest oil-yielding crop in plant kingdom and accumulates 90% of oil mesocarp. However, regulatory mechanisms lipid fatty acid (FA) metabolism are just beginning to be understood, more studies needed, especially understanding small noncoding RNA (ncRNA) mRNA. Based on deep sequencing RNAs degradome five developmental mesocarp stages, 452 microRNAs (miRNAs), including 170 conserved known-miRNAs (kn-miRNAs) 282 novel-miRNA (nov-miRNAs), were...
Coconut (Cocos nucifera L.) is one of the world's most versatile, economically important tropical crops. Little known about physiological and molecular basis coconut pulp (endosperm) development only a few genes gene product sequences are available in public databases. This study identified that were differentially expressed during functionally annotated these using bioinformatics analysis.
Nitrogen (N) starvation-induced triacylglycerol (TAG) synthesis, and its complex relationship with starch metabolism in algal cells, has been intensively studied; however, few studies have examined the interaction between amino acid TAG biosynthesis. Here, via a forward genetic screen for homeostasis, we isolated Chlamydomonas (Chlamydomonas reinhardtii) mutant (bkdE1α) that is deficient E1α subunit of branched-chain ketoacid dehydrogenase (BCKDH) complex. Metabolomics analysis revealed...
Coconut (Cocos nucifera L.) contains large amounts of medium chain fatty acids, which mostly recognise acyl-acyl carrier protein (ACP) thioesterases that hydrolyse acyl-ACP into free acids to terminate acyl elongation during acid biosynthesis. A full-length cDNA an thioesterase, designated CocoFatB1, was isolated from libraries prepared coconut endosperm fruit development. The gene contained open reading frame 1254 bp, encoding a 417-amino protein. amino sequence the CocoFatB1 showed 100%...
Summary Over 15 families of aquatic plants are known to use a strategy developmental switching upon environmental stress produce dormant propagules called turions. However, few molecular details for turion biology have been elucidated due the difficulties in isolating high-quality nucleic acids from this tissue. We successfully developed new protocol isolate transcripts and carried out RNA-seq analysis mature turions Greater Duckweed Spirodela polyrhiza . Comparison transcriptome that...