A5025683949- Malaria Research and Control
- Plant biochemistry and biosynthesis
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Calcium signaling and nucleotide metabolism
- Insect Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Trypanosoma species research and implications
- Lipid Membrane Structure and Behavior
- Metalloenzymes and iron-sulfur proteins
- Studies on Chitinases and Chitosanases
- Adenosine and Purinergic Signaling
- Insect symbiosis and bacterial influences
- Computational Drug Discovery Methods
- Parasite Biology and Host Interactions
- Electrocatalysts for Energy Conversion
- Signaling Pathways in Disease
- Genomics and Phylogenetic Studies
- Pharmacological Receptor Mechanisms and Effects
- Probiotics and Fermented Foods
- Ion channel regulation and function
- Pharmacological Effects of Natural Compounds
- Antibiotic Resistance in Bacteria
National Institute of Allergy and Infectious Diseases
2018-2023
National Institutes of Health
2018-2023
Government of the United States of America
2022
Birla Institute of Technology and Science, Pilani
2012-2019
Malaria parasites transmitted by mosquito bite are remarkably efficient in establishing human infections. The infection process requires roughly 30 minutes and is highly complex as quiescent sporozoites injected with saliva must be rapidly activated the skin, migrate through body, infect liver. This poorly understood for Plasmodium vivax due to low infectivity vitro models. To study this skin-to-liver-stage of malaria, we used quantitative bioassays coupled transcriptomics evaluate parasite...
Malaria parasites activate a broad-selectivity ion channel on their host erythrocyte membrane to obtain essential nutrients from the bloodstream. This conserved channel, known as plasmodial surface anion (PSAC), has been linked parasite clag3 genes in P. falciparum, but epigenetic switching between two copies of this gene hinders clear understanding how encoded protein determines PSAC activity. Here, we used linkage analysis falciparum cross where one parent carries single overcome effects...
Malaria parasites increase host erythrocyte permeability to ions and nutrients via a broad-selectivity channel known as the plasmodial surface anion (PSAC), linked parasite-encoded CLAG3 two associated proteins. These proteins lack multiple transmembrane domains typically present in channel-forming proteins, raising doubts about their precise roles. Using virulent human Plasmodium falciparum parasite, we report that undergoes self-association this protein's expression determines phenotype...
Malaria parasites grow within circulating red blood cells and uptake nutrients through a pore on their host membrane. Here, we used gene editing to tag CLAG3 RhopH2, two proteins linked the nutrient pore, with fluorescent markers tracked these in living infected cells. After synthesis mature parasites, imaging showed that both are packaged into membrane-bound rhoptries. When ruptured invaded new cells, were detected vacuole around parasite before they migrated inserted surface membrane of...
Our previous study identified 52 antiplasmodial peptaibols isolated from fungi. To understand their mechanism of action, we conducted phenotypic assays, assessed the in vitro evolution resistance, and performed a transcriptome analysis most potent peptaibol, HZ NPDG-I. NPDG-I 2 additional were compared for killing action stage dependency, each showing loss digestive vacuole (DV) content via ultrastructural analysis. demonstrated stepwise increase DV pH, impaired membrane permeability,...
OPINION article Front. Cell. Infect. Microbiol., 07 April 2022Sec. Parasite and Host https://doi.org/10.3389/fcimb.2022.881825
Malaria parasite harbors an essential prokaryotic-like organelle characterized by multiple mem-branes, akin to a plastid. This organelle, known as the apicoplast, evolved from secondary en-dosymbiosis. In this event, photosynthetic alga was engulfed eukaryotic host and subsequently transformed into symbiotic entity its free-living origin. As result, apicoplast remnant structure housing circular genome, enveloped four membranes. Concurrently, cell relinquished significant metabolic pathways,...
Introduction- Moderate environment is important to sustain life any condition that can be perceived as beyond the normal acceptable range extreme and if we are talking in terms of temperature than its called thermophillic environment.Aim & objective- The study was designed diversity thermophiles present compost characterization properties which responsible for their adequate nature high temperature. Material method- Microorganisms were isolated from cattle initially screened by...
Our previous study identified 52 antiplasmodial peptaibols isolated from Trichoderma and Hypocrea fungal species. To understand their mechanism of action, we conducted phenotypic assays, in vitro evolution resistance, transcriptome analysis the most potent peptaibol, harzianin NPDG I (HZ NPDG-I). This, two additional were compared for both killing action stage dependency, each showing a loss digestive vacuole (DV) content ultrastructural analysis. HZ-NPDG-I demonstrated stepwise increase DV...