A5103109313- Malaria Research and Control
- Parasitic Infections and Diagnostics
- Biological Research and Disease Studies
- Nanoparticle-Based Drug Delivery
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- CRISPR and Genetic Engineering
- Pregnancy and Medication Impact
- Computational Drug Discovery Methods
- Spondyloarthritis Studies and Treatments
- Spine and Intervertebral Disc Pathology
- Scoliosis diagnosis and treatment
- Axon Guidance and Neuronal Signaling
- Reproductive System and Pregnancy
- vaccines and immunoinformatics approaches
- Autoimmune and Inflammatory Disorders Research
- Bone and Joint Diseases
- Invertebrate Immune Response Mechanisms
- Endometriosis Research and Treatment
- Virus-based gene therapy research
- Aquaculture disease management and microbiota
- HIV/AIDS drug development and treatment
- CAR-T cell therapy research
- Hedgehog Signaling Pathway Studies
- Developmental Biology and Gene Regulation
University Health Network
2019
Krembil Research Institute
2019
Toronto Western Hospital
2017-2018
University of Toronto
2017-2018
Center for Global Health
2016-2018
University of South Florida
2016-2018
Memorial Sloan Kettering Cancer Center
2004
Howard Hughes Medical Institute
1989
Columbia University
1989
Severe malaria is caused by the apicomplexan parasite Plasmodium falciparum. Despite decades of research, distinct biology these parasites has made it challenging to establish high-throughput genetic approaches identify and prioritize therapeutic targets. Using transposon mutagenesis P. falciparum in an approach that exploited its AT-rich genome, we generated more than 38,000 mutants, saturating genome defining mutability fitness costs for over 87% genes. Of 5399 genes, our study defined...
In animals with binocular vision, retinal ganglion cell (RGC) axons from each eye sort in the developing ventral diencephalon to project ipsi- or contralateral targets, thereby forming optic chiasm. Ipsilaterally projecting arise ventrotemporal (VT) retina and contralaterally primarily other quadrants. The winged helix transcription factor Foxd1 (previously known as BF-2, Brain Factor 2) is expressed VT retina, well during formation of We report here that embryos lacking Foxd1,both...
Significance Artemisinin-based combination therapies (ACTs) are first-line antimalarial used worldwide. The artemisinin drug (ART) component clears the bulk of infection rapidly, but small numbers persistent parasites must be removed by partner drug. Longer parasite clearance t 1/2 values have been associated with a Kelch-propeller mutation (K13 C580Y), raising concerns increased ART resistance. We investigated effects C580Y using Plasmodium falciparum cross and monkey malaria model....
Malaria parasites transmitted by mosquito bite are remarkably efficient in establishing human infections. The infection process requires roughly 30 minutes and is highly complex as quiescent sporozoites injected with saliva must be rapidly activated the skin, migrate through body, infect liver. This poorly understood for Plasmodium vivax due to low infectivity vitro models. To study this skin-to-liver-stage of malaria, we used quantitative bioassays coupled transcriptomics evaluate parasite...
Plasmodium falciparum exhibits resistance to the artemisinin component of frontline antimalarial treatment Artemisinin-based Combination Therapy in South East Asia. Millions lives will be at risk if (ART-R) spreads Africa. Single non-synonymous mutations propeller region PF3D7_1343700,"K13" are implicated resistance. In this work, we use transcriptional profiling characterize a laboratory-generated k13 insertional mutant previously demonstrated have increased sensitivity artemisinins explore...
Though the P. falciparum genome sequence has been available for many years, ~40% of its genes do not have informative annotations, as they show no detectable homology to those studied organisms. More still evaluated via genetic methods. Scalable forward-genetic approaches that allow interrogation gene function without any pre-existing knowledge are needed hasten understanding parasite biology, which will expedite identification drug targets and development future interventions in face...
Objective The Psoriatic Arthritis Disease Activity Score ( PASDAS ) is a composite measure of psoriatic arthritis (PsA) disease activity. length its patient‐reported components raises concern about questionnaire burden. includes the Medical Outcomes Study Short Form 36 SF ‐36) health survey. We undertook this study to investigate agreement between and modified mPASDAS ), which substituted ‐36 with shortened ‐12. Methods A total 100 patients who fulfilled criteria Classification Group for PsA...