A5058355059- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- MicroRNA in disease regulation
- Thyroid Cancer Diagnosis and Treatment
- Circular RNAs in diseases
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Genomics and Chromatin Dynamics
- Peptidase Inhibition and Analysis
- Wnt/β-catenin signaling in development and cancer
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- Glutathione Transferases and Polymorphisms
- interferon and immune responses
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- BRCA gene mutations in cancer
Institute for Experimental Endocrinology and Oncology
2016-2025
University of Naples Federico II
2013-2025
National Research Council
2016-2025
European School of Molecular Medicine
2007-2023
University Hospital of Basel
2016-2020
University of Basel
2017
University of Campania "Luigi Vanvitelli"
2016
Creative Research Enterprises (United States)
2016
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2016
National Academies of Sciences, Engineering, and Medicine
2015-2016
MicroRNAs (miRNAs) are a class of small non-coding RNAs involved in wide range basic processes such as cell proliferation, development, apoptosis and stress response. It has recently been found that they also abnormally expressed many types human cancer. We analyzed the genome-wide miRNA expression profile thyroid papillary carcinomas (PTCs) using microarray (miRNACHIP microarray) containing hundreds precursor mature oligonucleotide probes. Using this approach, we an aberrant clearly...
We have recently reported that MicroRNAs (miR)-221 and miR-222 were up-regulated in human thyroid papillary carcinomas comparison with the normal tissue. Bioinformatic analysis proposed p27 Kip1 protein, a key regulator of cell cycle, as candidate target for miR-221/222 cluster. Here, we report enforced expression miR-221 was able to reduce protein levels carcinoma HeLa cells absence significant changes specific mRNA levels. This effect is direct negatively regulate 3′-untranslated...
The CBX7 gene encodes a polycomb group protein that is known to be downregulated in many types of human cancers, although the role this carcinogenesis remains unclear. To shed light on issue, we generated mice null for Cbx7. Mouse embryonic fibroblasts derived from these had higher growth rate and reduced susceptibility senescence compared with their WT counterparts. This was associated upregulated expression multiple cell cycle components, including cyclin E, which play key lung humans....
Micro-RNA have emerged as an important class of short endogenous RNA that act posttranscriptional regulators gene expression and are constantly deregulated in human cancer. MiR-1 has been found down-regulated lung, colon, prostate cancer.In this study, we investigated the possible role miR-1 thyroid carcinogenesis.We analyzed a panel neoplasias including benign malignant lesions searched for targets.Our results show is drastically adenomas carcinomas comparison with normal tissue....
Abstract Using gene expression profiling, we found that the CBX7 was drastically down-regulated in six thyroid carcinoma cell lines versus control cells. The aims of this study were to determine whether is related cancer phenotype and try identify new tools for diagnosis prognosis cancer. We thus evaluated various snap-frozen paraffin-embedded tissues different degrees malignancy by quantitative reverse transcription-PCR immunohistochemistry, respectively. progressively decreased with grade...
We have previously demonstrated that a set of micro-RNA (miRNA) is significantly down-regulated in anaplastic thyroid carcinomas with respect to normal tissues and differentiated carcinomas.The objective was evaluate the role two these miRNA, miR-25 miR-30d, carcinogenesis.miR-25 miR-30d expression restored ACT-1, 8505c, FRO cell lines, their effects on proliferation, migration, target were evaluated.We report polycomb protein enhancer zeste 2 (EZH2) has oncogenic activity drastically...
To evaluate the accuracy, consumable cost and time around testing (TAT) of a next-generation sequencing (NGS) assay, Ion Torrent AmpliSeq Colon Lung Cancer Panel, as an alternative to Sanger genotype KRAS, NRAS BRAF in colorectal cancer patients.The panel was first verified on cell lines control samples then prospectively applied routine specimens (n=114), with reference.The detected mutant alleles at 5% level correctly classified all tissues. The assay successfully carried out most (95.6%)...
Piscuoglio S, Zlobec I, Pallante P, Sepe R, Esposito F, Zimmermann A, Diamantis Terracciano L, Fusco A & Karamitopoulou E (2012) Histopathology 60, 397–404 HMGA1 and HMGA2 protein expression correlates with advanced tumour grade lymph node metastasis in pancreatic adenocarcinoma Aims: Pancreatic ductal follows a multistep model of progression through precursor lesions called intraepithelial neoplasia (PanIN). The high mobility group A1 (HMGA1) A2 (HMGA2) proteins are architectural...
High mobility group A (HMGA) overexpression plays a critical role in neoplastic transformation. To investigate whether HMGA acts by regulating the expression of microRNAs, we analyzed microRNA profile human breast adenocarcinoma cells (MCF7) transfected with HMGA1 gene, which results highly malignant phenotype. Among microRNAs induced HMGA1, focused on miR-181b, was overexpressed several neoplasias including carcinomas. We show that miR-181b regulates CBX7 protein levels, are down-regulated...
Chromobox protein homologue 7 (CBX7) is a chromobox family encoding novel polycomb protein, the expression of which shows progressive reduction, well related with malignant grade thyroid neoplasias. Indeed, CBX7 levels decreased in an increasing percentage cases going from benign adenomas to papillary, follicular, and anaplastic carcinomas. To elucidate function carcinogenesis, we searched for interacting proteins by proteomic analysis. By this approach, identified several proteins. Among...
// Valerio Costa 1,* , Roberta Esposito Carmela Ziviello 1 Romina Sepe 2 Larissa Valdemarin Bim Nunzio Antonio Cacciola Myriam Decaussin-Petrucci 3 Pierlorenzo Pallante Alfredo Fusco 2,4 and Ciccodicola 1,5 Institute of Genetics Biophysics “Adriano Buzzati-Traverso”, CNR, Naples, Italy Istituto per l’Endocrinologia e l’Oncologia Sperimentale (IEOS), Consiglio Nazionale delle Ricerche (CNR), c/o Dipartimento di Medicina Molecolare Biotecnologie Mediche (DMMBM),...
Anaplastic thyroid carcinoma (ATC) represents one the most aggressive neoplasias in humans, and, nowadays, limited advances have been made to extend survival and reduce mortality of ATC. Thus, identification molecular mechanism underlying its progression is needed. Here, we evaluated long non-coding RNA (lncRNA) expression profile nine ATC comparison with five normal tissues by a lncRNA microarray. By this analysis, identified 19 upregulated 28 downregulated lncRNAs fold change >1.1 or <-1.1...
HMGA1 is a non-histone nuclear protein that regulates cellular proliferation, invasion and apoptosis overexpressed in many carcinomas. In this study we sought to explore the expression of HCCs cirrhotic tissues, its effect vitro models.We evaluated using gene microarrays (59 HCCs, which 37 were matched with their corresponding tissue 5 normal liver donors) microarray (192 108 tissues 79 samples). was correlated clinicopathologic features patient outcome. Four cancer cell lines stable induced...
RNA-binding proteins (RBPs) are known to be involved in the initiation and development of malignant tumors, but roles RBPs esophageal cancer (EC) remain unclear. This study aims establish a prognostic signature based on through genome-wide analysis predict prognosis EC patients provide new insights into chemoresistance. The gene expression profiles clinical data with were downloaded from Xena database. Candidate genes obtained by taking intersection RBP genes, Kyoto Encyclopedia Genes...