Background Dengue imposes a substantial economic and disease burden in most tropical subtropical countries. incidence severity have dramatically increased Mexico during the past decades. Having objective comparable estimates of dengue is essential to inform health policy, increase awareness, assess impact prevention control technologies. Methods Findings We estimated annual for years 2010–2011. merged multiple data sources, including prospective cohort study; patient interviews macro-costing...

Abstract Zika virus (ZIKV) outbreak in Americas led to extensive efforts develop vaccines and ZIKV-specific diagnostics. In the current study, we use whole genome phage display library spanning entire ZIKV (ZIKV-GFPDL) for in-depth immune profiling of IgG IgM antibody repertoires serum urine longitudinal samples from individuals acutely infected with ZIKV. We observe a very diverse repertoire encompassing polyprotein on day 0 both urine. antibodies increase 10-fold between 7 serum, but not...

Dengue is currently the most widespread vector-borne disease, and its transmission has been intensively studied in endemic/hyperendemic localities. However, to obtain a complete picture of dengue transmission, it necessary study nonendemic Imported cases have reported Mexico City, presence eggs vector Aedes aegypti detected. In present study, we determined prevalence IgG antibodies against virus four city boroughs via random cluster sampling individuals aged 5-35 years. The weighted...

The activity of PERK, an endoplasmic reticulum (ER) transmembrane protein kinase, assists in ER stress response designed to inhibit general synthesis while allowing upregulated selective proteins such as the ATF4 transcription factor. PERK null mice exhibit phenotypes that especially affect secretory cell types. Although embryonic fibroblasts from these are difficult transfect with high efficiency, we have generated 293 cells stably expressing PERK-K618A dominant negative mutant....

Relatively few clues have been uncovered to elucidate the cell biological role(s) of mammalian ATP2C1 encoding an inwardly directed secretory pathway Ca2+/Mn2+ pump that is ubiquitously expressed. Deficiency results in a human disease (Hailey-Hailey), which primarily affects keratinocytes. ATP2C1-encoded protein detected Golgi complex calcium-dependent manner. A small interfering RNA causes knockdown expression, resulting defects both post-translational processing wild-type thyroglobulin (a...

Abstract Background Dengue is commonly considered an acute illness and follows three phases: febrile, critical in some cases recovery. However, a number of studies have described continuation dengue symptoms for weeks or months, extending the recovery phase. Here we evaluate this persistence during convalescence. Methods Our clinical cohort study included patients who sought medical services 48 to 144 h from onset fever at seven hospitals ambulatory centers Morelos, Mexico. Seventy-nine...

In single-chain insulins (SCIs), the C terminus of insulin B-chain is contiguous with N A-chain, connected by a short bioengineered linker sequence. SCIs have been proposed to offer potential benefit for gene therapy diabetes (Lee, H. C., Kim, S. J., K. S., Shin, and Yoon, J. W. (2000) Nature 408, 483–488) yet relatively little known about their folding, intracellular transport, or secretion from mammalian cells. Because can be considered as mutant proinsulin (with selective shortening...

The study of endemic dengue transmission is essential for proposing alternatives to impact its burden. traditional paradigm establishes that starts around cases, but there are few studies determine the risk.To assess association between peridomestic infection and exposure a index case (IC), cohort was carried out in two Mexican communities. People cohabitating with IC or living within 50-meter radius (exposed cohort) subjects areas no ICs 200-meter (unexposed were included.Exposure...

Abstract Background Aedes aegypti and albopictus are the main mosquito species responsible for dengue virus ( DENV ) transmission to humans in tropical subtropical regions of world. The role vertical epidemiology maintenance this arbovirus nature during interepidemic periods remain poorly understood, could sustain existence reservoirs within populations. Methods Between April 2011 October 2012, we monitored Ae. 9 cities 4 Mexican states. eggs were collected ovitraps, then adults reared under...

Dengue is one of the most important vector-borne diseases in world, causing significant morbidity and economic impact. In Colombia, dengue a major public health problem. Departments La Guajira, Cesar Magdalena are endemic areas. The objective this research to determine seroprevalence incidence virus infection participating municipalities from these Departments, also establish association between individual housing factors vector indices with incidence. We will assess knowledge, attitudes...

For thyroid hormone synthesis, peroxidase (TPO) molecules must be transported from the endoplasmic reticulum via Golgi complex to delivered at cell surface catalyze iodination of secreted thyroglobulin. Like other glycoproteins, TPO in transit have potential acquire endoglycosidase H resistance as a consequence Golgi-based modification their N-linked carbohydrates, and measurement intracellular distribution has often relied on this assumption. To examine thyrocyte lines, we prepared new...

Abstract Background Vector control programs, which have focused mainly on the patient house and peridomestic areas around dengue cases, not produced expected impact transmission. This project will evaluate assumption that endemic/epidemic transmission of begins vicinities primary cases. Its objective is to assess relationship between symptomatic case exposure infection incidence. Methods/Design A prospective cohort study be conducted (in Tepalcingo Axochiapan, in state Morelos, Mexico),...

Organisms' reactions to adverse events result in the generation of immune effectors, which, case insects, may be produced from direct activation pathways such as Toll, Jak-STAT, Imd, or RNAi derived crosstalk different intracellular pathways. One pathway, unfolded protein response (UPR), has primary objective restoring homeostasis endoplasmic reticulum. In addition, UPR participates signaling with pathways, generating protection against pathogenic organisms. Dengue virus is a plus-strand RNA...

We adapted the EQ-5D-3L questionnaire and visual analog scale to assess health-related quality of life (HRQOL) persistent symptoms in 79 patients with laboratory-confirmed dengue Morelos, Mexico. The lowest HRQOLs were 0.53 38.1 (febrile phase). Patients recovered baseline HRQOL ≈2 months.

Dengue is the most important arboviral disease in world. Seroprevalence has been proposed as a marker of endemicity, however, studies are scarce.We conducted cross-sectional, stratified cluster, random sample study to measure seroprevalence antibodies dengue virus (DENV) Mexico. The target population was school children ages 6-17 y from 22 endemic states Mexico, clustered four regions: Pacific, South-Central, Southeast and Low.A total 2134 subjects provided blood samples for immunoglobulin G...

Recommendations have been issued for vaccinating with the Sanofi Pasteur tetravalent dengue vaccine (CYD-TDV, Dengvaxia®) individuals aged from 9 to 45/60 years old a prior virus (DENV) infection and living in endemic countries/areas. One question linked these recommendations is determine when it possible start vaccination after laboratory confirmed wild-type DENV infection, this can be relevant any live used areas. To address it, we reviewed discussed immunological practical considerations...

The transmission pathways of dengue virus (DENV) among mosquitoes are a topic that has gained relevance in recent years because they could explain the maintenance wild independently human–mosquito horizontal cycle. In this regard, Aedes aegypti larvae exposed to supernatants C6/36 cells infected with DENV-4 were evaluated for excretion feces and viability infection immature stages (larvae). results demonstrate excrete their potential at least infect individuals same species. A pathway...