A5047673870- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Multiple Myeloma Research and Treatments
- Toxin Mechanisms and Immunotoxins
- SARS-CoV-2 detection and testing
- Poxvirus research and outbreaks
- Viral Infectious Diseases and Gene Expression in Insects
- Bacillus and Francisella bacterial research
- Monoclonal and Polyclonal Antibodies Research
- Olfactory and Sensory Function Studies
- Vaccine Coverage and Hesitancy
- Vector-borne infectious diseases
- Molecular Biology Techniques and Applications
- Heparin-Induced Thrombocytopenia and Thrombosis
- Neurobiology and Insect Physiology Research
- Viral-associated cancers and disorders
- Ear Surgery and Otitis Media
- Antifungal resistance and susceptibility
- Viral Infections and Vectors
- Immune responses and vaccinations
- Sinusitis and nasal conditions
Emory University
2020-2025
Tufts University
2010-2020
Omicron SARS-CoV-2 variants escape vaccine-induced neutralizing antibodies and cause nearly all current COVID-19 cases. Here, we compared the efficacy of three booster vaccines against BA.5 challenge in rhesus macaques: mRNA-1273, Novavax ancestral spike protein vaccine (NVX-CoV2373), or BA.1 version (NVX-CoV2515). All induced a strong cross-reactive binding antibody changed immunoglobulin G (Ig) dominance from IgG1 to IgG4 serum. also comparable responses multiple concern, including BQ.1.1,...
SARS-CoV-2 vaccines should induce broadly cross-reactive humoral and T cell responses to protect against emerging variants of concern (VOCs). Here, we inactivated the furin cleavage site (FCS) spike expressed by a modified vaccinia Ankara (MVA) virus vaccine (MVA/SdFCS) found that FCS inactivation markedly increased binding human ACE2. After vaccination mice, MVA/SdFCS induced eightfold higher neutralizing antibodies compared with MVA/S, which without inactivation, protected Beta variant. We...
Herpesviruses enter cells by membrane fusion either at the plasma or in endosomes, depending on cell type. Glycoprotein B (gB) is a conserved component of multiprotein herpesvirus machinery and functions as protein, with two internal loops, FL1 FL2. We determined crystal structures ectodomains mutants herpes simplex virus type 1 (HSV-1) gB to clarify whether their fusion-null phenotypes were due global local effects mutations structure ectodomain. Each mutant has single point mutation...
Abstract Background Mpox is an orthopoxvirus with a rodent reservoir that causes disease similar to mild smallpox. In 2022, sexually-transmitted global mpox outbreak infected over 90,000 individuals. Some immunocompromised patients experienced prolonged, severe resulting in significant morbidity, amputations, and death. People living HIV (PLWH) were at particularly high risk due impaired cell-mediated immunity, as CD4 CD8 T-cells help eradicate the virus from lesions. virion total binding...
To provide an accessible and inexpensive method to surveil for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations, we developed a multiplex real-time reverse transcription-PCR (rRT-PCR) assay, the Spike single-nucleotide polymorphism (SNP) detect specific mutations in spike receptor binding domain. A single primer pair was designed amplify 348-bp region of spike, probes were initially K417, E484K, N501Y. The assay evaluated using characterized variant sample pools...
Patients with multiple myeloma have unpredictable responses to vaccination for COVID-19. Anti-spike antibody levels can determine which patients develop antibodies at similar healthy controls, and are a known correlate of protection.A patient developed protective anti-spike after (608 IU/mL), but nonetheless severe breakthrough COVID-19 just 10 weeks following his second mRNA-1273.Sequencing the viral isolate revealed an extensively mutated variant spike protein mutations, including E484Q...
Abstract We report 3 complicated and prolonged cases of mpox in people with advanced human immunodeficiency virus (HIV) not on antiretroviral therapy (ART) at diagnosis. Multiple medical countermeasures were used, including tecovirimat treatment immune optimization ART initiation. Immunofluorescence skin biopsies demonstrated a dense infiltrate predominantly myeloid CD8+ T cells, strong type I interferon local response. RNAscope detected abundant replication monkeypox (MPXV) epithelial cells...
We have reported that IgG antibody responses following two mRNA COVID-19 vaccinations are impaired among patients with multiple myeloma (MM). In the current study, sixty-seven MM were tested for anti-spike antibodies 0-60 days prior to their first vaccination, 14-28 second dose, and both before after third dose of mRNA-1273 or BNT162b2 vaccines. After doses, most patients' (93 %) levels declined ineffective (<250 BAU/mL) (D3). D3 elicited in 84 % (61 full response 22 partial response). The...
Abstract Background Multiple myeloma (MM) patients have variable responses to mRNA vaccination COVID‐19. Little is known regarding their vaccine‐induced antibody levels over time. Methods We monitored spike IgG 24 weeks among a subset of 18 MM who showed full response after two vaccinations. Results had more rapid decline in as compared eight healthy controls, with power law half‐lives 72 days (vs. 107 days) and exponential 37 51 days). The longer SARS‐CoV‐2 were likely undetectable...
A 75-year-old immunocompetent male presented with a right orbital cellulitis after foreign body penetrating injury. He was taken for orbitotomy removal and started on broad-spectrum antibiotics. Intra-operative cultures were positive
Background Multiple myeloma (MM) patients have variable responses to mRNA vaccination COVID-19. Little is known regarding their vaccine-induced antibody levels over time. Methods We monitored spike IgG 24 weeks among a subset of 18 MM who showed full response after two vaccinations. had more rapid decline in as compared 8 healthy controls, with power law half-lives 72 days (versus 107 days) and exponential 37 51 days). Results The longer SARS-CoV-2 were likely undetectable monoclonal protein...
Abstract The family of olfactory receptors (ORs) subserves the sense smell and includes both functional alleles pseudogenes, latter identified by mutations resulting in frame shift or premature truncation. During neuronal differentiation, nonfunctional ORs are expressed initially but then switched out, and/or sensory neurons (OSNs) expressing them die. We carried out a transcriptomic analysis FACS-isolated cells from ΔSox2-eGFP , Neurog1-eGFP BAC ΔOMP-eGFP strains uninjured bulbectomized...
Abstract Powassan virus (POWV) is a tick-borne flavivirus which has resulted in increasing human cases over the past two decades. Despite high prevalence ticks and evidence of broad distribution North America, fewer than 50 are detected annually with undetected asymptomatic infections. Experimental studies relationships between POWV genetic diversity disease potential currently lacking. In present study, sixteen isolates originating from 13 locations United States Canada were used to assess...
Abstract Background Ebolaviruses cause viral hemorrhagic fever with high mortality rates. Nearly all Ebola vaccines in development use glycoprotein (GP) as the immunizing antigen. GP is present on membrane and functions cell entry by binding cellular receptor mediating fusion; antibodies to induce protective immunity. also produces sGP: a smaller, secreted form of containing receptor-binding domain; it able sGP naturally refolds after thermal denaturation thus may be more stable than GP,...