A5101729879- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Liver Diseases and Immunity
- Viral gastroenteritis research and epidemiology
- Pancreatitis Pathology and Treatment
- Disaster Management and Resilience
- COVID-19 epidemiological studies
- Virus-based gene therapy research
- Gut microbiota and health
- Immune responses and vaccinations
- Endoplasmic Reticulum Stress and Disease
- Genomics and Chromatin Dynamics
- Influenza Virus Research Studies
- Immune cells in cancer
- Liver Disease and Transplantation
- Diet, Metabolism, and Disease
- Infection Control and Ventilation
- Gallbladder and Bile Duct Disorders
Shantou University Medical College
2024
Nanjing Drum Tower Hospital
2020-2024
Nanjing University
2006-2024
Ragon Institute of MGH, MIT and Harvard
2021-2024
Massachusetts General Hospital
2024
Shantou University
2024
Nanjing Medical University
2020-2023
Shanghai Zhangjiang Laboratory
2014
The Omicron variant of SARS-CoV-2 has been shown to evade neutralizing antibodies elicited by vaccination or infection. Despite the global spread variant, even among highly vaccinated populations, death rates have not increased concomitantly. These data suggest that immune mechanisms beyond antibody-mediated virus neutralization may protect against severe disease. In addition pathogens, contribute control and clearance infections through Fc effector mechanisms. Here, we probed ability...
There is an urgent need for effective treatment and preventive vaccine to contain this devastating global pandemic, which requires a comprehensive understanding of humoral responses specific SARS-CoV-2 during the disease progression convalescent phase COVID-19 patients. We continuously monitored serum IgM IgG four related antigens, including nucleoprotein (NP), receptor binding domain (RBD), S1 protein, ectodomain (ECD) spike protein among non-severe severe patients seven weeks since onset....
The waning humoral immunity and emerging contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants resulted in the necessity of booster vaccination disease 2019 (COVID-19). inactivated vaccine, CoronaVac, is most widely supplied COVID-19 vaccine globally. Whether CoronaVac elicited adaptive responses that cross-recognize SARS-CoV-2 concern (VoCs) among 77 healthy subjects receiving third dose were explored. After boost, remarkable elevated spike-specific IgG IgA...
Summary Background Many patients with chronic hepatitis B (CHB) do not meet the definitions of traditional natural phases and are classified as being in grey zone (GZ). Aims To investigate liver histology, to establish a management strategy for CHB GZ. Methods This study included 1043 who underwent biopsy. Phases history were determined according AASLD 2018 guidance. GZ divided into HBeAg‐positive, normal ALT HBV DNA ≤10 6 IU/ml (GZ‐A); elevated ≤2 × 10 4 (GZ‐B); HBeAg‐negative, ≥2 3 (GZ‐C)...
Omicron SARS-CoV-2 variants escape vaccine-induced neutralizing antibodies and cause nearly all current COVID-19 cases. Here, we compared the efficacy of three booster vaccines against BA.5 challenge in rhesus macaques: mRNA-1273, Novavax ancestral spike protein vaccine (NVX-CoV2373), or BA.1 version (NVX-CoV2515). All induced a strong cross-reactive binding antibody changed immunoglobulin G (Ig) dominance from IgG1 to IgG4 serum. also comparable responses multiple concern, including BQ.1.1,...
Messenger RNA (mRNA) vaccines were highly effective against the ancestral SARS-CoV-2 strain, but efficacy of bivalent mRNA boosters XBB variants was substantially lower. Here, we show limited durability neutralizing antibody (NAb) responses and isotype switching to immunoglobulin G4 (IgG4) following boosting. Bivalent boosting elicited modest XBB.1-, XBB.1.5-, XBB.1.16-specific NAbs that waned rapidly within 3 months. In contrast, induced more robust sustained WA1/2020 suggesting immune...
Liver biopsies are recommended to exclude significant liver inflammation in patients with chronic hepatitis B (CHB) elevated HBV DNA but without other indications for antiviral treatment. We aimed investigate the proportions and determinants of Asian CHB detectable DNA. conducted a cross‐sectional study that retrospectively included 581 who had undergone biopsy. fibrosis were staged by Scheuer’s classification. Significant defined as G ≥ 2 S 2, respectively. There 179 (30.8%) alanine...
Background: The upper limits of normal (ULNs) for alanine aminotransferase (ALT) are different among international guidelines chronic hepatitis B (CHB). We aimed to investigate the proportion significant histological disease in Asian patients with CHB detectable virus (HBV) DNA under diverse ALT ULNs. Methods: Consecutive and HBV who underwent liver biopsy were retrospectively included from four tertiary hospitals. Above grade 2 inflammation stage fibrosis defined as fibrosis, respectively....
Immunotherapy was widely used for the treatment of non-small cell lung cancer (NSCLC). However, whether inhibition immune checkpoints individually or simultaneously could improve therapeutic efficacy NSCLC remains to be investigated. Here, we explored aberrant levels several and evaluated their potential diagnostic values NSCLC.Serum samples 89 patients 57 healthy donors were collected from Nanjing Drum Tower Hospital between November 2019 July 2020. Fourteen human quantified by...
The inactivated whole-virion vaccine, CoronaVac, is one of the most widely used coronavirus disease 2019 (COVID-19) vaccines worldwide. There a paucity data indicating durability immune response and impact imprinting induced by CoronaVac upon Omicron infection. In this prospective cohort study, 41 recipients triple-dose 14 unvaccinated individuals were recruited. We comprehensively profiled adaptive parameters in both groups, including spike-specific immunoglobulin (Ig) G IgA titers,...
Despite the dramatic spread of Omicron globally, even among highly vaccinated populations, death rates have not increased concomitantly. These data argue that alternative immune mechanisms, beyond neutralization, may continue to confer protection against severe disease. Beyond their ability bind and block infection, antibodies contribute control clearance multiple infections via direct antiviral immunity Fc-effector mechanisms. Thus, here we probed vaccine induced antibodies, across three...
Abstract Background The bivalent COVID-19 mRNA boosters became available in fall 2022 and were recommended alongside the seasonal influenza vaccine. However, immunogenicity of concurrent vs separate administration these vaccines remains unclear. Methods Here, we analyzed antibody responses health care workers who received booster vaccine on same day or different days through systems serology. Antibody-binding functional characterized at peak after 6 months following vaccination. Results IgG1...
Here we report two unrelated Chinese families with congenital missing teeth inherited in an X-linked manner. We mapped the affected locus to chromosome Xp11-Xq21 one family. In defined region, both were found have novel missense mutations ectodysplasin-A (EDA) gene. The mutation of c.947A>G caused D316G substitution EDA protein. c.1013C>T other family resulted Thr Met at position 338 EDA. gene has been reported responsible for hypohidrotic ectodermal dysplasia (XLHED) humans characterized by...
Abstract Introduction As an emerging infectious disease, coronavirus disease 2019 (COVID‐19) has rapidly spread throughout worldwide. Health care workers (HCWs) on frontline directly participated in the diagnosis, treatment, and of COVID‐19 patients are at high risk getting infected with highly severe acute respiratory syndrome 2 (SARS‐CoV‐2), novel that causes COVID‐19. In Nanjing Drum Tower Hospital, a total 222 medical staff went to Wuhan city for support. this study, we aimed determine...
The development of a safe and effective vaccine against SARS-CoV-2, the causative agent pandemic coronavirus disease-2019 (COVID-19), is global priority. Here, we aim to develop novel SARS-CoV-2 vaccines based on derivative less commonly used rare adenovirus serotype AdC68 vector. Three candidates were constructed expressing either full-length spike (AdC68-19S) or receptor-binding domain (RBD) with two different signal sequences (AdC68-19RBD AdC68-19RBDs). Single-dose intramuscular...
Global concerns arose as the emerged and rapidly spreading SARS-CoV-2 variants might escape host immunity induced by vaccination. In this study, a heterologous prime-boost immunization strategy for COVID-19 was designed to prime with DNA vaccine encoding wild type (WT) spike protein receptor-binding domain (RBD) followed S1 protein-based in rabbits. Four vaccine-elicited rabbit monoclonal antibodies (RmAbs), including 1H1, 9H1, 7G5, 5E1, were isolated biophysical property, neutralization...
Background Indices for predicting HBsAg or HBeAg seroconversion in patients with chronic hepatitis B virus (HBV) infection during antiviral therapy remain elusive.Objective We aimed to investigate if the presence of HBsAb-specific cells at baseline can predict HBV being treated peg-IFN-α nucleotide analogues (NAs).Methods 134 treatment-naive were enrolled study. collected peripheral blood mononuclear (PBMCs) from all and performed a cell ELISpot assay. Serum samples 12 weeks, 24 48 weeks...
Serum hepatitis B surface antigen (HBsAg) level < 100 IU/ml and undetectable virus (HBV) DNA have been recently proposed as an alternate endpoint of "partial cure" in chronic (CHB). We investigated clinical outcomes e (HBeAg)-negative CHB patients with HBsAg <100 HBV DNA. Treatment-naïve HBeAg-negative normal alanine aminotransferase were retrospectively included from three institutions. Patients classified into the low group (<100 IU/ml) high (≥100 IU/ml). Liver fibrosis was evaluated by...
Serum hepatitis B core antibody (anti-HBc) is associated with liver inflammation in chronic patients. This study aimed to investigate whether anti-HBc could serve as a predictor of significant e antigen (HBeAg)-negative virus (HBV) infected patients normal alanine aminotransferase (ALT) and detectable HBV DNA. Treatment-naïve HBeAg-negative ALT DNA who underwent biopsy were retrospectively included from two medical centers. Liver grade was evaluated using the Scheuer scoring system defined...