A5029637467- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Regulation of Appetite and Obesity
- Protein purification and stability
- Biochemical Analysis and Sensing Techniques
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Bone health and treatments
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Venous Thromboembolism Diagnosis and Management
- Adipose Tissue and Metabolism
- Advanced Radiotherapy Techniques
- Vitamin K Research Studies
- Amino Acid Enzymes and Metabolism
- Surgical Sutures and Adhesives
- RNA and protein synthesis mechanisms
- Vascular Procedures and Complications
- RNA Interference and Gene Delivery
- Receptor Mechanisms and Signaling
- Pancreatic function and diabetes
- Alkaline Phosphatase Research Studies
- Bone health and osteoporosis research
- Neurobiology and Insect Physiology Research
Texas A&M University
2024
University of Virginia
2002-2012
Eli Lilly (United States)
2001-2010
Weatherford College
2009
University of Hertfordshire
2008
Integra (United States)
2008
Merck (Singapore)
2007
Tokyo University of Agriculture and Technology
2006
Indianapolis Zoo
2006
University of Missouri
1981-2002
The peptide hormone ghrelin is the only known protein modified with an O-linked octanoyl side group, which occurs on its third serine residue. This modification crucial for ghrelin's physiological effects including regulation of feeding, adiposity, and insulin secretion. Despite role octanoylation in physiology ghrelin, lipid transferase that mediates this novel has remained unknown. Here we report identification characterization human GOAT, O-acyl transferase. GOAT a conserved orphan...
Little is known about leptin's interaction with other circulating proteins which could be important for its biological effects. Sephadex G-100 gel filtration elution profiles of 125I-leptin-serum complex demonstrated 125I-leptin eluting in significant proportion associated macromolecules. The binding to macromolecules was specific, reversible, and displaceable unlabeled leptin (ED50: 0.73 +/- 0.09 nM, mean SEM, n = 3). Several putative were detected by leptin-affinity chromatography either...
Lipoprotein lipase (LPL) is a key regulator of triglyceride clearance. Its coordinated regulation during feeding and fasting critical for maintaining lipid homeostasis energy supply. Angiopoietin-like (Angptl)3 Angptl4 are secreted proteins that have been demonstrated to regulate metabolism by inhibiting LPL. We taken targeted genetic approach generate Angptl4- Angptl3-deficient mice as well transgenic overexpressing human in the liver. The displayed elevated plasma triglycerides reduced...
Fibroblast growth factor-21 (FGF-21) is a metabolic regulator that can influence glucose and lipid control in diabetic rodents primates. We demonstrate betaKlotho an integral part of activated FGF-21-betaKlotho-FGF receptor (FGFR) complex thus critical subunit the FGF-21 receptor. Cells lacking did not respond to FGF-21; introduction these cells conferred FGF-21-responsiveness recapitulated entire scope signaling observed naturally responsive cells. Interestingly, FGF-21-mediated effects are...
A subset of nonsteroidal anti-inflammatory drugs (NSAIDs) has been shown to preferentially reduce the secretion highly amyloidogenic, 42-residue amyloid-beta peptide Abeta42. We found that Rho and its effector, Rho-associated kinase, regulated amount Abeta42 produced in vitro only those NSAIDs effective as inhibitors lowered Administration Y-27632, a selective Rock inhibitor, also brain levels transgenic mouse model Alzheimer's disease. Thus, Rho-Rock pathway may regulate amyloid precursor...
Pharmaceutical companies and regulatory agencies are broadly pursuing biomarkers as a means to increase the productivity of drug development. Quantifying differential levels proteins from complex biological samples such plasma or cerebrospinal fluid is one specific approach being used identify markers action, efficacy, toxicity, etc. We have developed comprehensive, fully automated, label-free relative protein quantification LC−MS/MS experiments proteolytic digests including: de-noising,...
The vitamin K-dependent bone protein matrix gamma-carboxyglutamic acid (Gla) (MGP) has been identified by radioimmunoassay in the guanidine extract of rat cartilage. MGP was present all cartilages tested at levels comparable to level bone. Western blot analysis indicated that molecular weight cartilage is same as MGP, and Northern revealed mRNA from size structurally related Gla could not be detected or analysis. discovery synthesized growth plate provide an explanation for excessive...
AMPA-type glutamate receptors (GluRs) mediate most excitatory signaling in the brain and are composed of GluR principal subunits transmembrane AMPA receptor regulatory protein (TARP) auxiliary subunits. Previous studies identified four mammalian TARPs, γ-2 (or stargazin), γ-3, γ-4, γ-8, that control trafficking, gating, pharmacology. Here, we explore roles for homologous γ-5 γ-7 proteins, which were previously suggested not to serve as TARPs. Western blotting reveals high levels cerebellum,...
The mechanism of matrix vesicle (MV) formation by growth plate chondrocytes in primary cell culture was assessed both using drugs which interfere with assembly or disassembly microfilaments and microtubules, as well comparison the composition chondrocyte microvilli MV. Cytochalasin D, is known to inhibit actin microfilaments, found stimulate release alkaline phosphatase-rich This stimulatory effect confirmed studies [3H]palmitate- 32P-prelabeled cells showed that cytochalasin D enhanced...
Human cerebrospinal fluid (CSF) is in direct contact with the brain extracellular space. Beside secretion of CSF by choroid plexus also derives directly from ependymal lining ventricular system and glial membrane blood vessels arachnoid. Therefore, biochemical change may be reflected CSF. a potential source protein molecular indices central nervous function pathology. However, various amounts contamination arise during sample acquisition. The concentration only 0.2 to 0.5% that blood. Minor...
Matrix vesicles (MV) can be readily isolated from culture media of chicken growth plate hypertrophic chondrocytes grown in primary culture. The maintain normal morphology and synthesize type II collagen throughout the period. culture-derived MV are morphologically indistinguishable seen situ rich alkaline phosphatase. Formation phosphatase-rich is strongly influenced by stage culture: large numbers released shortly after cell seeding; marked decline during spreading rapid division; notable...
A new method for measurement of transbilayer distribution sterol in plasma membranes is reported. The procedure utilized a fluorescent sterol, dehydroergosterol, and chemical quenching agent, trinitrobenzenesulfonic acid. Dehydroergosterol was useful as probe molecule sterols the following reasons. (a) Dehydro‐ ergosterol contained no bulky side chains reporter groups. (b) structurally resembled cholesterol desmosterol, primary synthesized by LM fibroblasts. (c) interacted with digitonin,...
Myosin light chain 1 (Myl3) is a 23-kDa isoform of one the subunits myosin, protein involved in muscle contraction. Myl3 presently being studied as biomarker cardiac necrosis to predict drug-induced cardiotoxicity, and work presented here, an LC/MS/MS assay was developed validated measure rat serum. The key steps this approach immunoaffinity purification from serum followed by on-bead digestion with trypsin release surrogate peptide. This tryptic peptide quantified using synthetic standard...
Osteoprotegerin (OPG) is a potent inhibitor of osteoclast formation and function. To elucidate how OPG regulated in bone, we examined (1) the expression localization protein bone tissue, (2) effect human parathyroid hormone 1-38 (hPTH 1-38) on messenger RNA (mRNA) levels rat femur metaphyseal diaphyseal (3) hPTH(1-38) mRNA cultured osteoblast-like cells derived from metaphysis diaphysis, ROS 17/2.8 osteosarcoma cells. Because PTH has been shown to stimulate osteoblast activity via cyclic...
Abstract Adipose tissue secretes leptin, which interacts with receptors in the hypothalamus. In rodent models of obesity, leptin increases metabolism and decreases food intake, helps to maintain normal body composition. Accurate precise methods quantitate circulating concentrations are needed for physiological studies. We developed an RIA measure rat plasma, serum, or adipocyte culture fluids. The working range assay, defined by detection limit highest calibrator, was 0.5–50 μg/L. Recovery...
We present a wrapper-based approach to estimate and control the false discovery rate for peptide identifications using outputs from multiple commercially available MS/MS search engines. Features of include flexibility combine output engines with sequence spectral derived features in flexible classification model produce score associated correct identifications. This reversed database is taken as null distribution estimating p-values rates simple established statistical procedure. Results 10...