A5008741937- RNA modifications and cancer
- Dialysis and Renal Disease Management
- Pancreatic and Hepatic Oncology Research
- RNA Research and Splicing
- Muscle and Compartmental Disorders
- Thyroid Cancer Diagnosis and Treatment
- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- RNA and protein synthesis mechanisms
- Renin-Angiotensin System Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Pancreatic function and diabetes
- Pancreatitis Pathology and Treatment
- Cancer-related gene regulation
- Apelin-related biomedical research
- S100 Proteins and Annexins
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Fibroblast Growth Factor Research
- Urticaria and Related Conditions
- Dermatology and Skin Diseases
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Gout, Hyperuricemia, Uric Acid
Martin Luther University Halle-Wittenberg
2015-2024
University Hospital in Halle
2007-2015
To increase the removal of middle-sized uremic toxins a new membrane with enhanced permeability and selectivity, called Medium Cut-Off (MCO-Ci) has been developed that at same time ensures retention albumin. Because many substances may contribute to micro-inflammation we hypothesized use MCO-Ci influences inflammatory state in hemodialysis patients.The randomized crossover trial 48 patients compared dialysis High-flux 4 weeks duration each plus 8 extension phase. Primary endpoint was gene...
CD97 knockdown impairs the metastatic capacity of SGC-7901 gastric cancer cells. However, role in distant lymphatic premetastatic niche formation remains unknown.Exosomes and soluble fraction were isolated from SGC-L (an SGC-7901-cell-derived highly cell line) CD97-knockdown (SGC-L/CD97-kd) cells, co-cultured with The two lines was evaluated vitro a footpad lymph node metastasis mouse model. Premetastatic-niche-formation-related proteins examined immunohistochemically.CD97 expression...
Increased levels of monocytic angiotensin-converting enzyme (ACE) found in haemodialysis (HD) patients may directly participate the pathogenesis atherosclerosis. We demonstrated recently that uremia triggers development highly pro-atherogenic monocytes via an angiotensin II (AngII)–dependent mechanism. Opposing actions AngII-degrading ACE2 remain largely unknown. examined status both ACEs and related receptors circulating leukocytes HD, not-dialyzed CKD healthy individuals. Furthermore, we...
Haemodialysis patients suffer from chronic systemic inflammation and high incidence of cardiovascular disease. One cause for this may be the failure diseased kidneys to eliminate immune mediators. Current haemodialysis treatment achieves insufficient elimination proteins in molecular weight range 15-45 kD. Thus, cut-off dialysis might improve inflammatory state.In randomized crossover trial, 43 were treated 3 weeks with or high-flux dialysis. Inflammatory plasma mediators, monocyte...
Lactate dehydrogenase A (LDHA) and Pyruvate Kinase M2 (PKM2) are important enzymes of glycolysis. Both them can be phosphorylated therefore regulated by Fibroblast growth factor receptor 1 (FGFR1). While phosphorylation LDHA at tyrosine10 leads to tetramerization activation, PKM2 tyrosine105 promotes dimerization inactivation. Dimeric is found in the nucleus regulates gene transcription. Up-regulation contribute faster proliferation under hypoxic conditions promote Warburg effect. Using...
Angiotensin-converting enzymes, ACE and ACE2, play not only a pivotal role in the regulation of blood pressure, but are involved processes pathophysiology, including thyroid dysfunction or progression several neoplasia such as cancers skin, lungs, pancreas leukemia. However, their carcinogenesis remains unknown. We examined this study expression ACE2 tissues possible employment biomarkers for cancer progression. Thyroid tissues, 14 goiters (G), 12 follicular adenomas (FA), 10 carcinomas...
Abstract Background Pancreatic cancer is one of the most aggressive human malignancies. Previous research has shown that periostin (POSTN) promotes pancreatic cell proliferation, migration, and invasion. Further, POSTN involved in tumor microenvironment remodeling during progression. However, relationship between expression, immune infiltration, efficacy immunotherapy unclear. Methods We conducted a comprehensive evaluation differential examining mRNA protein levels. To gather data, we...
CD97 is over-expressed in the majority of gastric adenocarcinomas and associated with its dedifferentiation aggressiveness. Our previous results demonstrated that out three isoforms tested, only small one was able to promote increased invasiveness vitro. Based on these data we further aimed investigate role isoform cancer progression vivo by employing cells a stable knock-down an orthotopic mouse model. We could demonstrate knock down CD97/EGF1,2,5, led significant decrease number...
<b><i>Background:</i></b> Decreased levels of leucocytic angiotensin converting enzyme 2 (ACE2) relate to atherosclerosis in patients with chronic kidney disease (CKD). Recently, micro RNA 421 (miR-421) was found target and down-regulate ACE2 human cardiac myofibroblasts. In this study, we investigated the correlation between serum miR-421 transcripts circulating leukocytes healthy individuals (NP), CKD (3–5) haemodialysis (HD) patients. Furthermore, tested possible...
Retinoic acid (RA) acts as an anti-proliferative and redifferentiation agent in the therapy of thyroid carcinoma. Our previous studies demonstrated that pretreatment follicular carcinoma cell lines FTC-133 FTC-238 resulted decreased vitro proliferation rates reduced tumor growth xenotransplants. In addition to results, we found RA led vitality invasiveness cells they reacted with reduction intracellular ATP levels number migrated respectively. However, molecular mechanisms by which mediates...
Aims Elevated expression levels of monocytic-ACE have been found in haemodialysis patients. They are not only epidemiologically linked with increased mortality and cardiovascular disease, but may also directly participate the initial steps atherosclerosis. To further address this question we tested role promotion atherosclerotic events vitro under conditions mimicking those chronic renal failure. Methods Results Treatment human primary monocytes or THP-1 cells uremic serum as well...
Vitamin C is one of the major extracellular nonenzymatic antioxidants involved in biosynthesis collagen. It promotes growth fibroblasts, wound healing processes, and enhances survival differentiation osteoblasts. The potential effects ascorbic acid on human dental pulp cells (DPC) apical papilla (CAP) used actual regenerative endodontic procedures remain largely unknown. In this study, we investigated possible employment therapies DPC CAP.Nine extracted wisdom teeth were selected for study....
The aim of this study was to clarify the clinical significance TM4SF members CD9, CD63 and CD82 in human gastric carcinoma.By employing RT-PCR immunohistochemistry, we studied expression 49 paired tissue specimens normal mucosa carcinoma. All tissues were obtained from patients who underwent curative surgery.All epithelium ulcer strongly expressed transcripts proteins as compared with corresponding controls. We found a significant correlation between mRNA level different pM statuses (P =...
AUF1/heterogeneous nuclear ribonucleoprotein D is an adenylate-uridylate-rich elements (AREs) -binding protein, which regulates the mRNA stability of many genes related to growth regulation, such as proto-oncogenes, factors, cytokines, and cell cycle-regulatory genes. Several studies demonstrated AUF1 involvement in processes apoptosis, tumorigenesis, development by its interactions with ARE-bearing mRNAs. We report here that may be involved thyroid carcinoma progression. Investigations on...
Hypertension is not only an integrative characteristic of hemodialysis (HD) patients but also very common in the general population. There evidence that inflammatory cytokine IL-β, regulated by NLRP3 inflammasome via caspase-1, contributes to hypertensive setting. Therefore, we investigated observational pilot study whether IL-1β secretion and cell death (pyroptosis) are different HD with intact kidney function. Twenty were age-, gender-, diabetes-mellitus-matched hypertension Caspase-1...
Introduction: Calcification is a highly relevant process in terms of development cardiovascular diseases, and its prevention may be the key to prevent disease progression patients. In this study we investigated expression osteocalcin (OC), osteopontin (OPN) RUNX2 patients’ leukocytes their possible role as diagnostic markers for diseases. Materials Methods: Leucocytes from 38 patients were collected Department Surgery Martin-Luther-University Halle, including 8 without arteriosclerotic...
CD82 (KAI1) and CD63 (ME491) are highly glycosylated proteins which belong to the transmembrane 4 superfamily (TM4SF). has been implicated as a possible prostate cancer metastasis suppressor gene, whereas is involved in progression of human melanoma cancer. Down-regulation both expression associated with metastatic potential several solid tumors. Currently, information lacking on role during thyroid carcinogenesis. The aim this study was determine whether useful prognostic indicator patients...
RNA-binding proteins may regulate every aspect of RNA metabolism, including pre-mRNA splicing, mRNA trafficking, stability and translation many genes. The dynamic association these with defines the lifetime, cellular localization, processing rate at which a specific is translated. One pathways involved in regulating mediated by adenylate uridylate-rich element (ARE) binding proteins. These are processes apoptosis, tumorigenesis development. Out ARE-binding proteins, two them AUF1 HuR were...
High mortality of haemodialysis patients is associated with systemic chronic inflammation and overactivation the renin–angiotensin system (RAS). Insufficient elimination pro-inflammatory immune mediators, especially in molecular weight range 15–45 kDa, may be one reasons for this. Employment membranes increased permeability was shown to ameliorate inflammatory response might modulate effects local RAS. In this study, we tested impact high cut-off (HCO), medium (MCO) high-flux (HF) dialysis...
Patients with chronic kidney disease maintained on intermittent hemodialysis suffer from systemic inflammation which is causally associated high mortality. Inflammation mediators of 15-45 kDa range cannot be effectively removed by conventional dialysis membranes. In this study, we tested the influence serum and dialysates obtained patients High cut-off or flux membranes profile THP-1 monocytes.THP-1 monocytes were treated within a randomized crossover pilot trial. Serum-treated cells...
Both adenylate-uridylate rich elements binding proteins AUF1 and HuR may participate in thyroid carcinoma progression.In this study we investigated the expression of both factors on a protein level with special focus follicular adenoma carcinoma.By employment immunofluorescence western blot 68 tissues including 7 goiter, 16 (4 adenomatous hyperplasia), 19 carcinomas, 13 papillary carcinomas 14 undifferentiated HuR.In addition to previous results demonstrated that are significantly...