A5070851743- Cancer Genomics and Diagnostics
- Colorectal Cancer Treatments and Studies
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Dialysis and Renal Disease Management
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Muscle and Compartmental Disorders
- Receptor Mechanisms and Signaling
- Radiomics and Machine Learning in Medical Imaging
- Head and Neck Cancer Studies
- Mast cells and histamine
- RNA modifications and cancer
- Asthma and respiratory diseases
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Phagocytosis and Immune Regulation
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Hedgehog Signaling Pathway Studies
- Central Venous Catheters and Hemodialysis
- Adolescent and Pediatric Healthcare
- Graphene and Nanomaterials Applications
University of Lübeck
2021-2022
University Hospital Schleswig-Holstein
2021
University Hospital Carl Gustav Carus
2011-2019
TU Dresden
2011-2019
German Cancer Research Center
2014-2018
Heidelberg University
2014-2018
University Medical Center Freiburg
2017-2018
University of Freiburg
2017-2018
Baxter (Germany)
2015-2017
King's College London
2015
Objective Gastric cancer is the second leading cause of cancer-related deaths and fifth most common malignancy worldwide. In this study, human mouse gastric organoids were generated to model disease perform drug testing delineate treatment strategies. Design Human organoid cultures established, samples classified according their molecular profile response conventional chemotherapeutics tested. Targeted was performed specific druggable mutations. Mouse carrying subtype-specific alterations....
To increase the removal of middle-sized uremic toxins a new membrane with enhanced permeability and selectivity, called Medium Cut-Off (MCO-Ci) has been developed that at same time ensures retention albumin. Because many substances may contribute to micro-inflammation we hypothesized use MCO-Ci influences inflammatory state in hemodialysis patients.The randomized crossover trial 48 patients compared dialysis High-flux 4 weeks duration each plus 8 extension phase. Primary endpoint was gene...
Drug-induced gene-expression profiles that invert disease have recently been illustrated to be a starting point for drug repositioning. In this study, we validate approach and focus on prediction of novel drugs colorectal cancer, which there is pressing need find antimetastatic compounds. We computationally predicted three still unknown compounds against cancer: citalopram (an antidepressant), troglitazone antidiabetic), enilconazole (a fungicide). verified the by in vitro assays clonogenic...
Like many other cancer patients, most pancreatic carcinoma patients suffer from severe weight loss. As shown in numerous studies with fish oil (FO) supplementation, a minimum daily intake of 1.5 g n-3-fatty acids (n-3-FA) contributes to stabilization and improvement quality life (QoL) patients. Given n-3-FA not as triglycerides (FO), but mainly bound marine phospholipids (MPL), QoL has already been seen at much lower doses (0,3 g), MPL were better tolerated. The objective this double-blind...
Abstract Purpose: Human papillomavirus (HPV)–negative head and neck squamous cell carcinoma (HNSCC) is associated with unfavorable prognosis, while independent prognostic markers remain to be defined. Experimental Design: We retrospectively performed miRNA expression profiling. Patients were operated for locally advanced HPV-negative HNSCC had received radiochemotherapy in eight different hospitals (DKTK-ROG; n = 85). Selection fulfilled comparable demographic, treatment, follow-up...
Haemodialysis patients suffer from chronic systemic inflammation and high incidence of cardiovascular disease. One cause for this may be the failure diseased kidneys to eliminate immune mediators. Current haemodialysis treatment achieves insufficient elimination proteins in molecular weight range 15-45 kD. Thus, cut-off dialysis might improve inflammatory state.In randomized crossover trial, 43 were treated 3 weeks with or high-flux dialysis. Inflammatory plasma mediators, monocyte...
Autophagy pathways promote the growth of pancreatic ductal adenocarcinoma (PDAC), but critical role is yet to be determined. Transcription factor EB (TFEB) centrally controls lysosomal and autophagy biogenesis. This study aimed explore TFEB for regulation in PDAC. We found that expression was significantly elevated human PDAC samples (n=45), localized cytoplasm nucleus 11 15 cases. In primary cell lines, nuclear evident even under basal conditions, further enrichment achieved by starvation....
Pancreatic cancer is a disease with high resistance to most common therapies and therefore has poor prognosis, which partly due lack of reaction apoptotic stimuli. Signal transduction such stimuli includes death receptor-mediated extrinsic pathway as well an intrinsic linked the mitochondria. Defects in pathways deregulation proteins, Survivin, Bcl-2, Bcl-xL Mcl-1, play decisive roles development pancreatic cancer. Investigation molecular mechanism allowing tumors resist cell would lead...
Organoid cultures of human pancreatic ductal adenocarcinoma (PDAC) have become a promising tool for tumor subtyping and individualized chemosensitivity testing. PDACs recently been grouped into different molecular subtypes with clinical impact based on cytokeratin-81 (KRT81) hepatocyte nuclear factor 1A (HNF1A). However, suitable antibody HNF1A is currently unavailable. The present study aimed at establishing in PDAC organoids using an alternative marker. A organoid biobank was generated...
The histamine H<sub>4</sub> receptor (H<sub>4</sub>R) is a classic pertussis toxin-sensitive G<sub>i</sub> protein–coupled that mediates increases in intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>). presence of H<sub>4</sub>R human eosinophils has been rigorously documented by several independent groups. It also suggested expressed monocytes, but this suggestion hinges part on antibodies with questionable specificity. This situation prompted us to reinvestigate expression...
// Kristin Werner 1 , Franziska Lademann May-Linn Thepkaysone Beatrix Jahnke Daniela E. Aust 2 Christoph Kahlert Georg Weber 3 Jürgen Weitz Robert Grützmann Christian Pilarsky Department of Visceral, Thoracic and Vascular Surgery, TU Dresden, 01307 Germany Institute Pathology, Universitätsklinikum Erlangen, 91054 Correspondence to: Pilarsky, e-mail: christian.pilarsky@uniklinikum-dresden.de Keywords: KRAS, apoptosis, RNAi, simultaneous gene silencing, pancreatic cancer Received: June 30,...
High mortality of haemodialysis patients is associated with systemic chronic inflammation and overactivation the renin–angiotensin system (RAS). Insufficient elimination pro-inflammatory immune mediators, especially in molecular weight range 15–45 kDa, may be one reasons for this. Employment membranes increased permeability was shown to ameliorate inflammatory response might modulate effects local RAS. In this study, we tested impact high cut-off (HCO), medium (MCO) high-flux (HF) dialysis...
Patients with chronic kidney disease maintained on intermittent hemodialysis suffer from systemic inflammation which is causally associated high mortality. Inflammation mediators of 15-45 kDa range cannot be effectively removed by conventional dialysis membranes. In this study, we tested the influence serum and dialysates obtained patients High cut-off or flux membranes profile THP-1 monocytes.THP-1 monocytes were treated within a randomized crossover pilot trial. Serum-treated cells...
Abstract Aims The Virtual Diabetes Outpatient Clinic for Children and Adolescents (VIDIKI) study was a 6-month quasi-randomized, multicentre followed by an extension phase to evaluate the effects of monthly video consultations in addition regular care. A health economic analysis conducted assess direct costs. Methods cost data 240 participants (1–16 years age) with type 1 diabetes who were already using continuous glucose monitoring system collected first 6 months study. intervention group...
The acute effects of drinks rich in protein (PRO) versus carbohydrate (CHO) on cardiovascular hemodynamics and reactivity are uncertain. A randomized crossover design was used to compare 400-mL isoenergetic (1.1 MJ) containing whey (PRO; 44 g) or (CHO; 57 400 mL water 14 healthy men. primary secondary outcomes were changes cardiac output, blood pressure, systemic vascular resistance (SVR) digital volume pulse measured prior 30 min following consumption at rest, during 12 multi-stage bicycle...
Introduction: We focus on the expression analysis of non-coding microRNAs (miRNAs) with regulatory functions in breast cancer (BC), especially intrinsic subgroup characterized as triple negative (TNBC). Objective interdisciplinary collaboration is establishment a distinct miRNA signature panel diagnostic and prognostic tool TNBC management.