A5079383491- Radiation Therapy and Dosimetry
- Cancer, Hypoxia, and Metabolism
- Head and Neck Cancer Studies
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Diagnosis and Treatment
- Advanced Radiotherapy Techniques
- Effects of Radiation Exposure
- Medical Imaging Techniques and Applications
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- Lung Cancer Research Studies
- Cancer Research and Treatments
- Lung Cancer Treatments and Mutations
- Nuclear Physics and Applications
- Cancer-related Molecular Pathways
- Gamma-ray bursts and supernovae
- Radiopharmaceutical Chemistry and Applications
- Radiation Effects in Electronics
- Cancer Cells and Metastasis
- PARP inhibition in cancer therapy
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Advances in Oncology and Radiotherapy
- Machine Learning in Materials Science
University of Duisburg-Essen
2020-2024
German Cancer Research Center
2014-2023
TU Dresden
2014-2023
Helmholtz-Zentrum Dresden-Rossendorf
2016-2023
University Hospital Carl Gustav Carus
2014-2023
OncoRay
2014-2023
Heidelberg University
2014-2023
Deutschen Konsortium für Translationale Krebsforschung
2014-2023
Essen University Hospital
2023
West German Heart and Vascular Center Essen
2022
Objective Gastric cancer is the second leading cause of cancer-related deaths and fifth most common malignancy worldwide. In this study, human mouse gastric organoids were generated to model disease perform drug testing delineate treatment strategies. Design Human organoid cultures established, samples classified according their molecular profile response conventional chemotherapeutics tested. Targeted was performed specific druggable mutations. Mouse carrying subtype-specific alterations....
To investigate the impact of hypoxia-induced gene expression and cancer stem cell (CSC) marker on outcome postoperative cisplatin-based radiochemotherapy (PORT-C) in patients with locally advanced head neck squamous carcinoma (HNSCC).Expression CSC markers CD44, MET, SLC3A2, hypoxia signatures were analyzed resected primary tumors using RT-PCR nanoString technology a multicenter retrospective cohort 195 patients. CD44 protein was further tissue microarrays. Primary endpoint locoregional...
Abstract MALDI mass spectrometry imaging (MALDI imaging) uniquely advances cancer research, by measuring spatial distribution of endogenous and exogenous molecules directly from tissue sections. These molecular maps provide valuable insights into basic translational including tumor biology, microenvironment, biomarker identification, drug treatment, patient stratification. Despite its advantages, is underutilized in studying rare cancers. Sarcomas, a group malignant mesenchymal tumors, pose...
To measure the effect of acute oxygen depletion on cell survival for different types radiation, experiments have been performed using Chinese hamster ovary (CHO) cells and RAT-1 rat prostate cancer cells. A special chamber has developed to perform irradiations under levels oxygenation. The concentrations used were normoxia (air), hypoxia (94.5% N2, 5% CO2, 0.5% O2) anoxia (95% CO2). Cells exposed X-rays C-, N- or O-ions with linear energy transfer (LET) values ranging from 100-160 keV/µm....
Abstract Purpose: The aim of this study was to identify and independently validate a novel gene signature predicting locoregional tumor control (LRC) for treatment individualization patients with locally advanced HPV-negative head neck squamous cell carcinomas (HNSCC) who are treated postoperative radio(chemo)therapy (PORT-C). Experimental Design: Gene expression analyses were performed using NanoString technology on multicenter training cohort 130 an independent validation 121 patients....
Background and purposeRadiomics analyses have been shown to predict clinical outcomes of radiotherapy based on medical imaging-derived biomarkers. However, the biological meaning attached such image features often remains unclear, thus hindering translation radiomics analysis. In this manuscript, we describe a preclinical trial, which attempts establish correlations between expression histological tumor microenvironment (TME)- magnetic resonance imaging (MRI)-derived features.Materials &...
Little is known about the early pathogenic events by which mutant superoxide dismutase 1 (SOD1) causes amyotrophic lateral sclerosis (ALS). This lack of mechanistic understanding a major barrier to development and evaluation efficient therapies. Although protein aggregation be involved, it not understood how SOD1 degeneration motoneurons (MNs). Previous research has relied heavily on overexpression SOD1, but clinical relevance models remains questionable. We used human induced pluripotent...
Radiation-induced late side effects such as cognitive decline and normal tissue complications can severely affect quality of life outcome in long-term survivors brain tumors. Proton therapy offers a favorable depth-dose deposition with the potential to spare tumor-surrounding tissue, thus potentially reducing effects. In this study, we describe preclinical model reveal underlying biological mechanisms caused by precise high-dose proton irradiation subvolume. We studied dose- time-dependent...
Purpose: In order to take full advantage of proton radiotherapy, the biological effect protons in normal and tumor tissue should be investigated understood detail.The ongoing discussion on variable relative effectiveness along depth dose distribution (eg, Paganetti 2015), also administration concomitant treatments, demands dedicated vitro trials that prepare translation into clinics.Therefore, a setup for radiobiological studies corresponding dosimetry established enables experiments at...
Proton radiotherapy has been implemented into the standard-of-care for cancer patients within recent years. However, experimental studies investigating cellular and molecular mechanisms are lacking, prognostic biomarkers needed. Cancer stem cell (CSC)-related biomarkers, such as aldehyde dehydrogenase (ALDH), known to influence radiosensitivity through inactivation of reactive oxygen species, DNA damage repair, death. In a previous study, we found that ionizing radiation itself enriches...
We previously established annexin A2 as a radioresponsive protein associated with anchorage independent growth in murine epidermal cells. In this study, we demonstrate nuclear translocation human skin organotypic culture and cells after exposure to X radiation (10-200 cGy), supporting conserved function for A2. Whole genome expression profiling the presence absence of [shRNA] identified fundamentally altered transcriptional programming that changes transcriptome. Bioinformatics predicted...
The relative biological effectiveness (RBE) of particle therapy compared to photon radiotherapy is known be variable but the exact dependencies are still subject debate. In vitro data suggested that RBE a large extend independent ion type if parametrized by beam quality Q. This study analyzed dependence pre-clinical on late toxicity with an emphasis quality.Published dose-response spinal cord following one and two fractions carbon irradiation were compiled. for each treatment condition was...
To independently validate the impact of tumour volume, p16 status, cancer stem cell (CSC) marker expression and hypoxia-associated gene signatures as potential prognostic biomarkers for patients with locally advanced head neck squamous carcinoma (HNSCC), who underwent primary radiotherapy or radiochemotherapy (RCTx). These markers have previously been reported in a study German Cancer Consortium Radiation Oncology Group (DKTK-ROG) (Linge et al., 2016).In this retrospective monocentric study,...