A5011137826- Genetic Associations and Epidemiology
- Pancreatic and Hepatic Oncology Research
- Genomics and Rare Diseases
- Pancreatitis Pathology and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Nanoparticle-Based Drug Delivery
- Extracellular vesicles in disease
- Gastric Cancer Management and Outcomes
- BRCA gene mutations in cancer
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Optical Coatings and Gratings
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Photonic and Optical Devices
- Renal and related cancers
- Renal cell carcinoma treatment
- Glycosylation and Glycoproteins Research
- MRI in cancer diagnosis
- Tuberous Sclerosis Complex Research
- Gallbladder and Bile Duct Disorders
- Receptor Mechanisms and Signaling
- Lipid metabolism and disorders
Second Affiliated Hospital of Zhejiang University
2012-2025
Regeneron (United States)
2019-2024
Division of Program Coordination Planning and Strategic Initiatives
2022
National Applied Research Laboratories
2010-2020
Zhejiang University
2008-2018
University at Albany, State University of New York
2017
Shaoxing People's Hospital
2017
Scripps Research Institute
2013
Tongji University
2011
Shenyang Jianzhu University
2005-2010
Abstract A major goal in human genetics is to use natural variation understand the phenotypic consequences of altering each protein-coding gene genome. Here we used exome sequencing 1 explore protein-altering variants and their 454,787 participants UK Biobank study 2 . We identified 12 million coding variants, including around loss-of-function 1.8 deleterious missense variants. When these were tested for association with 3,994 health-related traits, found 564 genes trait associations at P ≤...
The UK Biobank is a prospective study of 502,543 individuals, combining extensive phenotypic and genotypic data with streamlined access for researchers around the world
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters human host cells via angiotensin-converting enzyme (ACE2) and causes disease 2019 (COVID-19). Here, through a genome-wide association study, we identify variant (rs190509934, minor allele frequency 0.2-2%) that downregulates ACE2 expression by 37% (P = 2.7 × 10-8) reduces the risk of SARS-CoV-2 infection 40% (odds ratio 0.60, P 4.5 10-13), providing genetic evidence levels influence COVID-19 risk. We also replicate...
SUMMARY The UK Biobank is a prospective study of 502,543 individuals, combining extensive phenotypic and genotypic data with streamlined access for researchers around the world. Here we describe first tranche large-scale exome sequence 49,960 participants, revealing approximately 4 million coding variants (of which ~98.4% have frequency < 1%). includes 231,631 predicted loss function variants, >10-fold increase compared to imputed same participants. Nearly all genes (>97%) had ≥1...
To investigate the mechanism underlying promoting role of CD97 in gastric cancer cell proliferation and invasion.Two types exosomes released by cells with high (SGC/wt) or low (SGC/kd) expression were isolated ultracentrifugation identified electron microscopy western blot analysis. The influences two on invasion investigated Matrigel assays. Exosomal miRNAs subsequently from samples their miRNA profiles compared via microarray assay Reverse transcription-quantitative real-time polymerase...
The Mexico City Prospective Study is a prospective cohort of more than 150,000 adults recruited two decades ago from the urban districts Coyoacán and Iztapalapa in
Metabolic syndrome (MetS) is a difficult-to-manage disease that poses significant risk to human health. Here, we show the supplementation of Lactobacillus reuteri ZJ617 ameliorates symptoms MetS in mice induced by high-fat diet. L. modulates host metabolism interacting with microbiome, resulting production spermidine synthesized microbiota. serves as source substrates for microbiota synthesize spermidine, hence preventing decline bacteria responsible production. Spermidine treatment mimics...
CD97 knockdown impairs the metastatic capacity of SGC-7901 gastric cancer cells. However, role in distant lymphatic premetastatic niche formation remains unknown.Exosomes and soluble fraction were isolated from SGC-L (an SGC-7901-cell-derived highly cell line) CD97-knockdown (SGC-L/CD97-kd) cells, co-cultured with The two lines was evaluated vitro a footpad lymph node metastasis mouse model. Premetastatic-niche-formation-related proteins examined immunohistochemically.CD97 expression...
Abstract The UK Biobank Exome Sequencing Consortium (UKB-ESC) is a unique private/public partnership between the and eight biopharma companies that will sequence exomes of all ∼500,000 participants. Here we describe early results from exome data generated by this consortium for first ∼200,000 UKB subjects key features project enabled UKB-ESC to come together generate data. sequencing 200,643 enrollees are now accessible research community. Approximately 10M variants were observed within...
Chilaiditi syndrome refers to a medical condition that is indicated by the presence of sign, radiological observation colonic interposition between liver and diaphragm, associated with other clinical symptoms. rare entity therefore, often misdiagnosed in practice, however, it may be accompanied series severe complications, such as bowel obstruction perforation. The current study describes 47-year-old male who presented repeated abdominal pain acute intestinal obstruction. patient was...
Retroperitoneal liposarcoma is a rare tumor with an incidence of 2.5 per million individuals. Early diagnosis difficult as there absence specific clinical presentations. The present case study reports patient diagnosed retroperitoneal who was treated by complete surgical resection and relapsed 3 months following the surgery. In addition, data 14 patients were reviewed analyzed. mean age at presentation 54.1 (range, 36-73 years) 5/14 experienced recurrence, ranging between 1 10 times. Of 12...
Background Cardiometabolic diseases are highly comorbid, but their relationship with female-specific or overwhelmingly female-predominant health conditions (breast cancer, endometriosis, pregnancy complications) is understudied. This study aimed to estimate the cross-trait genetic overlap and influence of burden cardiometabolic traits on unique women. Methods Results Using electronic record data from 71 008 ancestrally diverse women, we examined relationships between 23...
Lacrimal gland (LG) morphogenesis and repair are regulated by a complex interplay of intrinsic factors (e.g., transcription factors) extrinsic signals soluble growth/signaling factors). Many these interconnections remain poorly characterized. Runt-related (Runx) belong to small family heterodimeric known regulate lineage-specific proliferation differentiation stem cells. The purpose this study was define the expression pattern role Runx proteins in LG development regeneration.Expression...
Patient-derived organoids (PDO) have been proposed as a novel in vitro method of drug screening for different types cancer. However, to date, extrahepatic biliary tract carcinoma (eBTC) PDOs not yet fully established.We collected six samples gallbladder (GBC) and one sample cholangiocarcinoma (eCCA) from seven patients attempt establish eBTC screening. We successfully established five GBC eCCA PDOs. Histological staining was used compare structural features between the original tissues...
PURPOSE Breast and ovarian tumors in germline BRCA1/2 carriers undergo allele-specific loss of heterozygosity, resulting homologous recombination deficiency (HRD) sensitivity to poly-ADP-ribose polymerase (PARP) inhibitors. This study investigated whether biallelic HRD also occur primary nonbreast/ovarian that arise carriers. METHODS A clinically ascertained cohort with a cancer was identified, including canonical (prostate pancreatic cancers) noncanonical (all other) tumor types....
CD97 is over-expressed in the majority of gastric adenocarcinomas and associated with its dedifferentiation aggressiveness. Our previous results demonstrated that out three isoforms tested, only small one was able to promote increased invasiveness vitro. Based on these data we further aimed investigate role isoform cancer progression vivo by employing cells a stable knock-down an orthotopic mouse model. We could demonstrate knock down CD97/EGF1,2,5, led significant decrease number...
Glaucoma is a leading cause of blindness. Current glaucoma medications work by lowering intraocular pressure (IOP), risk factor for glaucoma, but most treatments do not directly target the pathological changes to increased IOP, which can manifest as medication resistance disease progresses. To identify physiological modulators we performed genome- and exome-wide association analysis in >129,000 individuals with IOP measurements extended these findings an risk. We report identification...
KLF4 is implicated in tumor progression of pancreatic cancer, but the molecular regulatory mechanism needs to be further specified. We aimed probe malignant cancer. qRT-PCR or western blot was completed test levels predicted genes. Dual-luciferase and chromatin immunoprecipitation (ChIP) assays were designed validate binding between Cell viability oncogenicity detection used for vitro vivo functional assessment. a downstream target miR-135b-5p. could regulate GPRC5A level. MiR-135b-5p...
Abstract Background Up to one of every six individuals diagnosed with cancer will be a second primary in their lifetime. Genetic factors contributing the development multiple cancers, beyond known syndromes, have been underexplored. Methods To characterize genetic susceptibility we conducted pan-cancer, whole-exome sequencing study drawn from two large multi-ancestry populations (6429 cases, 165,853 controls). We created groupings cancers: (1) an overall combined set at least cancers across...
The management of ovarian teratomas in normal conditions is well established, but rare giant cases (tumor diameter over 15 cm), the choice management, such as laparotomic or laparoscopic approaches, are controversial and may be therapeutically challenging for surgeons. aims current study were to analyze clinical features teratoma discuss its management. data 330 patients with (of whom 1 patient was treated by authors 329 admitted Second Affiliated Hospital Zhejiang University Medical College...
Gastric cancer is the fourth most common malignancy globally. In order to decrease dosage and side effects of conventional chemotherapy, achieve improved benefits from molecular targeted therapy, novel drug delivery systems were developed in present study. Oxaliplatin-Au-Fe3O4-Herceptin® acts as a dual-functional nanoparticles (NPs) conjugate possesses capability human epithelial growth factor receptor 2 (HER2) targeting oxaliplatin delivery. The 8-20 nm Au-Fe3O4 synthesized by decomposing...