A5057757930- Gut microbiota and health
- Animal Behavior and Welfare Studies
- Clostridium difficile and Clostridium perfringens research
- Reproductive System and Pregnancy
- Probiotics and Fermented Foods
- Systemic Lupus Erythematosus Research
- Animal Nutrition and Physiology
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Immune Response and Inflammation
- Adolescent and Pediatric Healthcare
- Pharmacological Effects of Natural Compounds
- Pediatric health and respiratory diseases
- Viral gastroenteritis research and epidemiology
- Single-cell and spatial transcriptomics
- Alcohol Consumption and Health Effects
- Polyamine Metabolism and Applications
- Animal Virus Infections Studies
- Neonatal Respiratory Health Research
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Clinical Nutrition and Gastroenterology
- Drug-Induced Hepatotoxicity and Protection
- Viral Infections and Immunology Research
Zhejiang University
2020-2025
Virginia–Maryland College of Veterinary Medicine
2019-2023
Virginia Tech
2019-2023
Zhejiang A & F University
2019-2020
Metabolic syndrome (MetS) is a difficult-to-manage disease that poses significant risk to human health. Here, we show the supplementation of Lactobacillus reuteri ZJ617 ameliorates symptoms MetS in mice induced by high-fat diet. L. modulates host metabolism interacting with microbiome, resulting production spermidine synthesized microbiota. serves as source substrates for microbiota synthesize spermidine, hence preventing decline bacteria responsible production. Spermidine treatment mimics...
Significance Infants are born without an established gut microbiota, which develops rapidly after birth and is shaped by the maternal microbiota. However, how through shaping neonatal would affect establishment of a strong immune system in neonates remains unclear. Here, we show mechanistically microbiota regulates de novo production IgA.
Abstract Background Aflatoxins have been reported as a significant pollutant in feed, capable of causing harm to the liver, gastrointestinal tract and kidneys piglets. However, research on interactions among aflatoxin B1 (AFB1), bile acid (BA) metabolism gut microbiota is limited. Methods In this study, piglets were treated with AFB1 antibiotics (ABX) evaluate interaction between microbiota. Subsequently, roles farnesoid X receptor (FXR) sterol 12α-hydroxylase ( CYP8B1 ) absorption studied...
Autoimmune diseases, such as systemic lupus erythematosus, are characterized by excessive inflammation in response to self-antigens. Loss of appropriate immunoregulatory mechanisms contribute disease exacerbation. We previously showed the suppressive effect vancomycin treatment during "active-disease" stage lupus. In this study, we sought understand same given before onset. To develop a model which test regulatory role gut microbiota modifying autoimmunity, treated lupus-prone mice with...
Dysbiosis of gut microbiota exists in the pathogenesis many autoimmune diseases, including systemic lupus erythematosus (lupus). Lupus patients who experienced pregnancy usually had more severe disease flares post-delivery. However, possible role link between and exacerbation remains to be explored. In classical mouse model MRL/lpr, we compared structures pregnant lactating individuals vs. age-matched naïve mice. Consistent with studies on non-lupus mice, both lactation significantly changed...
Wild pigs usually showed high tolerance and resistance to several diseases in the wild environment, suggesting that gut bacteria of could be a good source for discovering potential probiotic strains. In our study, pig feces were sequenced higher relative abundance genus Lactobacillus (43.61% vs. 2.01%) than domestic pig. A total 11 lactic acid (LAB) strains including two L. rhamnosus, six mucosae, one fermentum, delbrueckii, Enterococcus faecalis species isolated. To investigate synergistic...
Current live rotavirus vaccines are costly with increased risk of intussusception due to vaccine replication in the gut vaccinated children. New improved safety and cost-effectiveness needed. In this study, we assessed immunogenicity protective efficacy a novel P24-VP8* nanoparticle using gnotobiotic (Gn) pig model human infection disease. Three doses (200 μg/dose) intramuscular Al(OH)3 adjuvant (600 μg) conferred significant protection against diarrhea after challenge virulent Wa strain...
Abstract Background In mammals, transitioning from sole milk uptake to the intake of solid feed results in dramatic developmental changes intestinal function and immunological status. fact, weaning stress is often accompanied by inflammatory processes. To develop effective intervention strategies, it necessary characterize pattern immune response that occurs on weaning, as we have done this study for piglets. Results comprehensively delineate cell heterogeneity ileum tissues underlying...
Intrauterine growth restriction (IUGR), when a fetus does not grow as expected, is associated with reduction in hepatic functionality and higher risk for chronic liver disease adulthood. Utilizing early developmental plasticity to reverse the outcome of poor fetal programming remains an unexplored area. Focusing on biochemical profiles neonates previous transcriptome findings, piglets from same are selected models studying IUGR. The cellular landscape created by scRNA-seq reveal...
Abstract Background This study investigated the protective effects of L. reuteri ZJ617 on intestinal and liver injury underlying mechanisms in modulating inflammatory, autophagy, apoptosis signaling pathways a piglet challenged with lipopolysaccharide (LPS). Methods Duroc × Landrace Large White piglets were assigned to 3 groups ( n = 6/group): control (CON) LPS received oral phosphate-buffered saline for 2 weeks before intraperitoneal injection (i.p.) physiological or (25 μg/kg body weight),...
Abstract MRL/lpr mice have been extensively used as a murine model of lupus. Disease progression in can differ among animal facilities, suggesting role for environmental factors. We noted phenotypic drift our in-house colony, which was the progeny obtained from The Jackson Laboratory (JAX; stocking number 000485), that involved attenuated glomerulonephritis, increased splenomegaly, and reduced lymphadenopathy. To validate lupus, we compared these with those newly JAX, were confirmed to be...
NLRP12 has dual roles in shaping inflammation. We hypothesized that would modulate myeloid cells and T cell function to control systemic autoimmunity. Contrary our hypothesis, the deficiency of Nlrp12 autoimmune-prone B6. Fas lpr/lpr mice ameliorated autoimmunity males but not females. dampened B terminal differentiation, germinal center reaction, survival autoreactive leading decreased production autoantibodies reduced renal deposition IgG complement C3. In parallel, expansion potentially...
Tributyrin and essential oils have been used as alternatives to antimicrobials improve gut health growth performance in piglets. This study was evaluate the effects of a dietary supplement with two encapsulated products containing different combinations tributyrin oregano or methyl salicylate on performance, serum biochemical parameters related physiological status, intestinal microbiota metabolites A total 108 weaned crossbred piglets (Yorkshire × Landrace, 21 ± 1 d, 8.21 0.04 kg) were...
Nursing mom can regulate the gut microbiome succession in offspring. However, it remains unclear whether these effects are long-term and what effect has on growth performance of piglets. This study aimed to develop a cross-fostering model piglets investigate maternal environment microbiota, even offspring, if this could be maintained long term.Four groups were generated as follows (n = 12): Duroc nursed by their birth (Dd) or Yorkshire sows (Yd), (Dy) (Yy). The found that improved for time....
Abstract To investigate the ability of gut microbiota to modify immune regulation in autoimmune disease, we treated lupus-prone mice with oral vancomycin prior disease development, and subsequently administered bacterial DNA isolated from commensal bacteria. Vancomycin given initiation promotes exacerbation likely due a decrease frequency B regulatory (Breg) cells, whereas after onset has suppressive effects on phenotype MRL/lpr mice. Bacterial administration during pre-disease stage...
Abstract Systemic lupus erythematosus (SLE) is a multi-system autoimmune disease. The cause of SLE not only genetic; in fact, environmental factors may play more important role disease development. MRL/lpr mouse model lupus-like symptoms due to multiple susceptible loci the MRL background, and offers an accelerated compared parent strain Faslpr mutation. Recently, our laboratory witnessed loss phenotype in-house colony mice. We thus mice newly obtained from Jackson Laboratory (JAX; Stock...