As the global population continues to grow, competition for resources between humans and livestock has been intensifying. Increasing milk protein production improving feed efficiency are becoming increasingly important meet demand high-quality dairy protein. In a previous study, we found that yield in cows was associated with rumen microbiome. The objective of this study elucidate potential microbial features underpins using metagenomics, metatranscriptomics, metabolomics.Comparison...

Abstract Background Dairy cows utilize human-inedible, low-value plant biomass to produce milk, a low-cost product with rich nutrients and high proteins. This process largely relies on rumen microbes that ferment lignocellulose cellulose volatile fatty acids (VFAs). The VFAs are absorbed partly metabolized by the stratified squamous epithelium, which is mediated diverse cell types. Here, we applied metagenomic binning approach explore individual involved in fiber digestion performed...

Over the years, microbiome research has achieved tremendous advancements driven by culture-independent meta-omics approaches. Despite extensive research, our understanding of functional roles and causal effects on phenotypes remains limited. In this study, we focused rumen metaproteome, combining it with metatranscriptome metabolome data to accurately identify active distributions microorganisms specific groups that influence feed efficiency. By integrating host genetics data, established...

Metabolic syndrome (MetS) is a difficult-to-manage disease that poses significant risk to human health. Here, we show the supplementation of Lactobacillus reuteri ZJ617 ameliorates symptoms MetS in mice induced by high-fat diet. L. modulates host metabolism interacting with microbiome, resulting production spermidine synthesized microbiota. serves as source substrates for microbiota synthesize spermidine, hence preventing decline bacteria responsible production. Spermidine treatment mimics...

Lactobacillus plantarum (L. plantarum) has been globally regarded as antibiotic alternative in animal farming the past few years. However, potential function of L. broilers not systemically explored. In this study, a total 560 one-day-old yellow-feathered were randomly divided into 3 groups, fed with basal diet and drank HJZW08 (LP) at concentration 0 (CON), 1000 × 10^5 (LP1000), 2000 CFU/L (LP2000) for 70 d. Results showed that body weight (BW), average daily gain (ADG), feed intake (ADFI),...

Mastitis is an inflammatory disease, affects the dairy industry and has a severe economic impact. During subclinical mastitis, milk production quality deteriorates. Recently, rumen microbial composition been linked to health, but few studies have investigated effect of microbiota on mammary health in cows. This study was undertaken identify associated fermentation cows with different somatic cell counts (SCC), speculation that statuses gland bacterial diversity. A total 319 Holstein fed same...

With the increasing demand for enhancing livestock production performance and optimizing feed efficiency, this study aimed to investigate effects of fermented total mixed ration (FTMR) containing different proportions rice straw sheath leaves Zizania latifolia on systemic nutrient metabolism oxidative under host genetic regulation growth heifers. A 157 heifers aged 7-8 months were selected, their hair was collected whole-genome sequencing. They randomly assigned into four groups 18 21 cattle...

Wild pigs usually showed high tolerance and resistance to several diseases in the wild environment, suggesting that gut bacteria of could be a good source for discovering potential probiotic strains. In our study, pig feces were sequenced higher relative abundance genus Lactobacillus (43.61% vs. 2.01%) than domestic pig. A total 11 lactic acid (LAB) strains including two L. rhamnosus, six mucosae, one fermentum, delbrueckii, Enterococcus faecalis species isolated. To investigate synergistic...

Mastitis is one of the major problems for productivity dairy cows and its classifications have usually been based on milk somatic cell counts (SCCs). In this study, we investigated differences in production, rumen fermentation parameters, diversity composition hindgut bacteria with similar SCCs aim to identify whether they can be potential microbial biomarkers improve diagnostics mastitis. A total 20 over 500 × 103 cells/mL but without clinical symptoms mastitis were selected study. Random...

Abstract Background Antimicrobial resistance is one of the most urgent threat to global public health, as it can lead high morbidity, mortality, and medical costs for humans livestock animals. In ruminants, rumen microbiome carries a large number antimicrobial genes (ARGs), which could disseminate environment through saliva, or flow microbial biomass hindgut released feces. The occurrence distribution ARGs in microbes has been reported, revealing effects external stimuli (e.g.,...

Background: Intermittent fasting (IF) can reduce energy intake and body weight (BW). Melatonin has many known functions, which include reducing appetite preventing excessive gain. Objective: This study aimed to investigate the effects of IF on fat gut microbiota metabolome as well a potential interaction with melatonin. Methods: Male C57BL/6J mice (23.0 ± 0.9 g, 6 wk old) were randomly assigned into four groups (12 mice/group): control (C), intermittent (F), melatonin (M), plus (MF). The C M...

Abstract Background In mammals, transitioning from sole milk uptake to the intake of solid feed results in dramatic developmental changes intestinal function and immunological status. fact, weaning stress is often accompanied by inflammatory processes. To develop effective intervention strategies, it necessary characterize pattern immune response that occurs on weaning, as we have done this study for piglets. Results comprehensively delineate cell heterogeneity ileum tissues underlying...

Mangrove forests inhabit tropical or subtropical intertidal zones and have remarkable abilities in coastline protection. Kandelia obovata is considered the most cold-tolerant mangrove species has been widely transplanted to north zone of China for ecological restoration. However, physiological molecular mechanisms K. under colder climate was still unclear. Here, we manipulated typical cold waves with cycles cold/recovery analyzed transcriptomic responses seedlings. We found that both traits...

The beneficial effects of butyric acid in poultry production are well documented, while the relationship between sodium butyrate (SB) and microcapsule sustained-release (MSSB), especially yellow broilers, remains poorly investigated. This study was designed to elucidate function as potential mechanisms SB MSSB enhancing health broilers. In total, 360 one-day-old broilers were allocated three treatment groups. control group (CON) received a basic diet, provided with 1000 mg/kg (SB), all over...

Abstract Background This study investigated the protective effects of L. reuteri ZJ617 on intestinal and liver injury underlying mechanisms in modulating inflammatory, autophagy, apoptosis signaling pathways a piglet challenged with lipopolysaccharide (LPS). Methods Duroc × Landrace Large White piglets were assigned to 3 groups ( n = 6/group): control (CON) LPS received oral phosphate-buffered saline for 2 weeks before intraperitoneal injection (i.p.) physiological or (25 μg/kg body weight),...

Transforming the gut microbiota has turned into most intriguing target for interventions in multiple gastrointestinal and non-gastrointestinal disorders. Fecal transplantation (FMT) is a therapeutic tool that administers feces collected from healthy donors patients to help replenish microbial balance. Considering random donor selection, maintain optimal ecosystem, post-FMT critical therapy outcomes but challenging. Aiming study of different diets on recipients' originated with diets, we...

Abstract Background Restoration through planting is the dominant strategy to conserve mangrove ecosystems. However, many of plantations fail survive. Site and seeding selection matters for planting. The process afforestation, where individuals were planted in a novel environment, essentially human-controlled transplanting events. Trying deepen expand understanding effects on plants, we have performed seven-year-long reciprocal transplant experiment Kandelia obovata along latitudinal...

Both fecal microbiota transplantation (FMT) and dietary fiber intervention were verified as effective ways to manipulate the gut microbiota, whereas little is known about influence of combined methods on microbiota. Here, we constructed "non-industrialized" "industrialized" models investigate donor effect FMT diet in shaping Mice transplanted from domestic pig received a with low-fiber (D) or high-fiber (DF), other two groups wild then (W) (WF), respectively. Gut WF mice showed lower Shannon...