A5018715904- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Force Microscopy Techniques and Applications
- Wound Healing and Treatments
- 3D Printing in Biomedical Research
- Wnt/β-catenin signaling in development and cancer
- Lipid Membrane Structure and Behavior
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
- Mesenchymal stem cell research
- Galectins and Cancer Biology
- Connective tissue disorders research
- Exercise and Physiological Responses
- Tendon Structure and Treatment
- Viral Infections and Immunology Research
- Gut microbiota and health
- Natural Products and Biological Research
- Dendrimers and Hyperbranched Polymers
- Advances in Oncology and Radiotherapy
- Genomics and Phylogenetic Studies
- Heat shock proteins research
- Cardiomyopathy and Myosin Studies
- Angiogenesis and VEGF in Cancer
- Systemic Lupus Erythematosus Research
- Pressure Ulcer Prevention and Management
Georgia Institute of Technology
2014-2024
Vanderbilt University Medical Center
2024
Vanderbilt University
2023
The Wallace H. Coulter Department of Biomedical Engineering
2014-2017
Cornell University
2011-2015
Emory University
2015
Massachusetts Institute of Technology
2013
Abstract Stem cell therapies are limited by poor survival and engraftment. A hurdle to the use of materials for delivery is lack understanding material properties that govern transplanted stem functionality. Here, we show synthetic hydrogels presenting integrin-specific peptides enhance survival, persistence, osteo-reparative functions human bone marrow-derived mesenchymal cells (hMSCs) in murine defects. Integrin-specific regulate hMSC adhesion, paracrine signaling, osteoblastic...
Focal adhesions (FAs) regulate force transfer between the cytoskeleton and ECM-integrin complexes. We previously showed that vinculin regulates transmission at FAs. Vinculin residence time in FAs correlated with applied force, supporting a mechanosensitive model which forces stabilize vinculin's active conformation to promote transfer. In present study, we examined relationship traction vinculin-paxillin localization single context of substrate stiffness actomyosin contractility. found...
Resolution of intestinal inflammation and wound repair are active processes that mediate epithelial healing at mucosal surfaces. Lipid molecules referred to as specialized proresolving mediators (SPMs) play an important role in the restorative response. Resolvin E1 (RvE1), a SPM derived from omega-3 fatty acids, has been reported dampen by promoting anti-inflammatory responses including increased neutrophil spherocytosis macrophage production IL-10. Despite these observations, for RvE1...
Abstract How adhesive forces are transduced and integrated into biochemical signals at focal adhesions (FAs) is poorly understood. Using cells adhering to deformable micropillar arrays, we demonstrate that traction force FAK localization as well Y397-FAK phosphorylation linearly coupled individual FAs on stiff, but not soft, substrates. Similarly, increases with external applied using magnetic beads. This mechanosignaling coupling requires actomyosin contractility, talin-FAK binding,...
Vinculin binding to actin filaments is thought be critical for force transduction within a cell, but direct experimental evidence support this conclusion has been limited. In the present study, we found mutation (R1049E) of vinculin tail impairs its ability bind F-actin, stimulate polymerization, and bundle F-actin in vitro. Further, mutant expressing cells are altered cell migration, which accompanied by changes adhesion, spreading generation traction forces, providing role...
We report a key role for Dsc2 in simple epithelial cell migration and mucosal wound healing vivo using newly generated mice with inducible conditional knockdown of intestinal cells ( Villin-Cre ERT2 ; fl/fl ).
Abstract Progress in our understanding of mechanotransduction events requires noninvasive methods for the manipulation forces at molecular scale physiological environments. Inspired by cellular mechanisms force application (i.e. motor proteins pulling on cytoskeletal fibers), we present a unique machine that can apply cell-matrix and cell-cell junctions using light as an energy source. The key actuator is light-driven rotatory linked to polymer chains, which intercalated between membrane...
Cell adhesion to the extracellular matrix (ECM) involves integrin receptor-ligand binding and clustering form focal (FA) complexes, which mechanically link cell's cytoskeleton ECM regulate fundamental cell signaling pathways. Although elucidation of biochemical events in cell-matrix adhesive interactions is rapidly advancing, recent studies show that forces underlying are also critical responses. Therefore, multiple measurement systems have been developed quantify spatial temporal dynamics...
Focal adhesions (FAs) link the cytoskeleton to extracellular matrix and as such play important roles in growth, migration, contractile properties of vascular smooth muscle cells. Recently, it has been shown that downregulation Nox4, a transforming growth factor (TGF) β-inducible, hydrogen peroxide (H2O2)-producing enzyme, affects number FAs. However, effectors downstream Nox4 mediate FA regulation are unknown. The resident protein H2O2-inducible clone (Hic)-5 is H2O2 TGFβ inducible, binding...
Basement membranes (BMs) are specialised extracellular matrices that provide structural support to tissues as well influence cell behaviour and signalling. Mutations in COL4A1/COL4A2, a major BM component, cause familial form of eye, kidney cerebrovascular disease, including stroke, while common variants these genes risk factor for intracerebral haemorrhage the general population. These phenotypes associated with matrix defects, due mutant protein incorporation and/or its absence by...
Abstract Background Oscillating x‐ray attenuation in the lungs provides an opportunity to evaluate pulmonary perfusion without contrast. Recent intensity‐based methods have been compared scintigraphy and CT angiography but lack rigorous phantom studies. Purpose A new method quantify periodic signal amplitude was employed using spectral analysis. Performance characterized a water capable of creating oscillating at physiologic amplitudes. Feasibility detecting abnormal performed on volunteer...
Abstract Mechanotransduction events in physiological environments are difficult to investigate, part due the lack of experimental tools apply forces mechanosensitive receptors remotely. Inspired by cellular mechanisms for force application (i.e. motor proteins pulling on cytoskeletal fibers), here we present a unique molecular machine that can at cell-matrix and cell-cell junctions using light as an energy source. The key actuator is light-driven rotatory linked polymer chains, which...
Abstract How adhesive forces are transduced and integrated into biochemical signals at focal adhesions (FAs) is poorly understood. Using cells adhering to deformable micropillar arrays, we demonstrate that traction force FAK localization as well Y397-FAK phosphorylation linearly coupled individual FAs on stiff, but not soft, substrates. Similarly, increases with external applied using magnetic beads. This mechanosignaling coupling requires actomyosin contractility, talin-FAK binding,...
Heat stroke (HS) is an important civilian and military medical problem. Characterizing the cellular responses to HS critical for improved diagnosis treatment of HS. Commonly used in vitro heat “shock” models inadequately represent physiological stressors experienced by cells during vivo . The objective this study was create a high‐precision system dynamically expose cultured core‐temperature (T c ; radiotelemetry) profiles exhibited mice recovery. We assembled consisting Peltier plate,...
Chronisch entzündliche Darmerkrankungen führen zu Ulzerationen und Zerstörung der intestinalen epithelialen Barriere. Das klinische Ausmaß hängt mit pathologischen Freisetzung pro-inflammatorischer Mediatoren wie Platelet Activating Factor (PAF) Tumor Necrosis alpha (TNF-a) zusammen. TNF-a neutralisierende Antikörper werden zur erfolgreichen Remissionsinduktion angewendet, wobei es jedoch bei einer signifikanten Patientenzahl zum Therapieversagen kommt, potentiell durch Inhibition...